Research Advances in Multi-Omics on the Traditional Chinese Herb Dendrobium officinale

Abstract

Dendrobium officinale Kimura et Migo is an important epiphytic plant, belonging to the Orchidaceae family. There are various bioactive components in D. officinale plants, mainly including polysaccharides, alkaloids, and phenolic compounds. These compounds have been demonstrated to possess multiple functions, such as anti-oxidation, immune regulation, and anti-cancer. Due to serious shortages of wild resources, deterioration of cultivated germplasm and the unstable quality of D. officinale, the study has been focused on the biosynthetic pathway and regulation mechanisms of bioactive compounds. In recent years, with rapid developments in detection technologies and analysis tools, omics research including genomics, transcriptomics, proteomics and metabolomics have all been widely applied in various medicinal plants, including D. officinale. Many important advances have been achieved in D. officinale research, such as chromosome-level reference genome assembly and the identification of key genes involved in the biosynthesis of active components. In this review, we summarize the latest research advances in D. officinale based on multiple omics studies. At the same time, we discuss limitations of the current research. Finally, we put forward prospective topics in need of further study on D. officinale.

Probiotic fermentation improves the bioactivities and bioaccessibility of polyphenols in Dendrobium officinale under in vitro simulated gastrointestinal digestion and fecal fermentation

“…As a well-known homologous medicine and food worldwide, DO has been reported to contain abundant active ingredients such as alkaloids, polysaccharides, phenanthrenes, anthrones, sesquiterpenoids, polyphenols, etc., and demonstrate many biological activities including antioxidant, antitumor, hypoglycemic, and immunomodulatory activities (3). However, most of the studies focused on the common bioactive component in DO such as polysaccharides and alkaloids, whereas minimal studies were carried on its polyphenols (PPs), another kind of important secondary metabolites (4)(5)(6). Previous studies reported the abundant PPs in DO, such as rutin, quercitrin, hyperoside, and quercetin, that were classified as flavonoids.…”

Traditional processing increases biological activities of Dendrobium offificinale Kimura et. Migo in Southeast Yunnan, China

“…In order to promote further research into FSD and TPFD, attentions should be paid to the following work in the future: (1) Although current metabolites technologies have been used to study TPFD, there are limitedly comprehensive metabonomics studies. Obviously, the negative or positive ion model method such as metabolomics cannot satisfy the necessary deep research into D. officinale 21 . (2) it is important to comprehensively investigate why differences exist in active compounds between FSD and TPFD which can provide propitiate conditions for metabolite accumulation.…”

“…Previous studies of the chemical composition of D. officinale have mainly focused on elucidating the botany, traditional use, phytochemistry, and pharmacology of D. officinale . While some studies have examined properties pertaining to the quality and safety of this herb 20 and omics studies have explored the biosynthetic pathways and regulation mechanisms of the plant’s bioactive compounds 21 , the major chemical differences between raw and processed TPFD products are largely unknown. Exploring the chemical changes that occur during D. officinale processing will therefore provide helpful information for understanding the therapeutic characteristics of TPFD.…”

Identification of the carotenoid cleavage dioxygenase genes and functional analysis reveal DoCCD1 is potentially involved in beta-ionone formation in Dendrobium officinale

“…The bioactive effects of beta-ionone such as anti-cancer have been demonstrated ( Paparella et al, 2021 ). D. officinale has been used as a medicinal, edible, and ornamental plant, and is rich in polysaccharides, phenols, and terpene compounds ( Tang et al, 2017 ; Wang Y. et al, 2022 ). In our present study, among the volatile components in the stem and leaf, the relatively abundant beta-ionone was found ( Figure 2 ), which was similar to the previous report ( Ma et al, 2018 ).…”

“…Dendrobium officinale is a traditional medicinal plant ( Ng et al, 2012 ). Modern studies have shown that there are abundant bioactive compounds, such as alkaloids, bibenzyl, polysaccharides, and phenolic compounds ( Tang et al, 2017 ; Wang Y. et al, 2022 ). However, there is limited knowledge on the biosynthesis of beta-ionone.…”