Traditional processing increases biological activities of Dendrobium offificinale Kimura et. Migo in Southeast Yunnan, China

Abstract

The orchid Dendrobium officinale grows throughout southeast China and southeast Asian countries and is used to treat inflammation and diabetes in traditional Chinese medicine. Tie pi feng dou is a well-known traditional Chinese medicine made from the dried D. officinale stems. Processing alters the physicochemical properties of TPFD; however, it is unclear how processing affects the quality and medicinal value of this plant. Here, we analyzed and compared the chemical composition of fresh stems of D. officinale and TPFD and explored possible explanations for the enhanced medicinal efficacy of processed D. officinale stems using qualitative and quantitative methods. To identify the components of FSD and TPFD, we used ultra-high-performance liquid chromatography combined with mass spectrometry in negative and positive ion modes and interpreted the data using the Human Metabolome Database and multivariate statistical analysis. We detected 23,709 peaks and identified 2352 metabolites; 370 of these metabolites were differentially abundant between FSD and TPFD (245 more abundant in TPFD than in FSD, and 125 less abundant), including organooxygen compounds, prenol lipids, flavonoids, carboxylic acids and their derivatives, and fatty acyls. Of these, 43 chemical markers clearly distinguished between FSD and TPFD samples, as confirmed using orthogonal partial least squares discriminant analysis. A pharmacological activity analysis showed that, compared with FSD, TPFD had significantly higher levels of some metabolites with anti-inflammatory activity, consistent with its use to treat inflammation. In addition to revealing the basis of the medicinal efficacy of TPFD, this study supports the benefits of the traditional usage of D. officinale.

Comparison of the Antioxidant Activities and Polysaccharide Characterization of Fresh and Dry Dendrobium officinale Kimura et Migo

“…It was shown that traditional processing would change the contents of the key metabolites and improve the biological activities of DO [ 39 ]. Therefore, we further investigated the differences in monosaccharide composition between the FDOP and DDOP ( Table 2 ).…”

Environment-responsive dendrobium polysaccharide hydrogel embedding manganese microsphere as a post-operative adjuvant to boost cascaded immune cycle against melanoma

“…Dendrobium has been used for anti-inflammation in traditional Chinese medicine. DOP, a key bioactive component of dendrobium, can inhibit oxidative stress and alleviate oxidative stress-induced damage 43 , 44 . These properties suggest that DOP may reduce oxidative stress-induced injury in the post-surgical environment.…”

Integrating artificial intelligence into the modernization of traditional Chinese medicine industry: a review

“…The processing of TCM is a pharmaceutical technology based on the theory of TCM, syndrome differentiation, the inherent properties of drugs and the unique requirements for compounding and preparation. The purpose is to retain therapeutic ingredients while eliminating ineffective or potentially harmful ingredients (Zhou et al, 2022). TCM is mainly administered in the form of slices or pieces.…”