Rescue of Rous Sarcoma Virus in Mixed Cultures of Virogenic Mammalian and Chicken Cells, Treated and Untreated with Sendai Virus and Detected by Focus Assay

Svoboda, Jan; Machala, O; Deozánek, T

doi:10.1099/0022-1317-2-3-461

Cited by 39 publications

(12 citation statements)

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“…Virus tests and virus rescue procedures. Transforming viruses were assayed by focus assay in 6 cm polystyrene tissue culture dishes (Kooh-i-noor, Hardtmuth, Dalecin, Czechoslovakia) using the schedule described earlier (Svoboda et al, 1968) and expressed as focus-forming units (f.f.u.). Virus rescue procedures were performed as described previously (Svoboda,t981).…”

Section: Cells and Virusesmentioning

confidence: 99%

Characterization of Transforming Viruses Rescued from a Hamster Tumour Cell Line Harbouring the v-src Gene Flanked by Long Terminal Repeats

Geryk¹,

Pichrtová²,

Guntaka³

et al. 1986

Journal of General Virology

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SUMMARY"The organization of proviruses derived from infecting transforming viruses rescued from hamster tumour cells was studied. Southern blot analysis indicated that the provirus from the F6 cell line was organized as long terminal repeat (LTR)-src-LTR, and S1 mapping experiments suggested that it was probably derived by reverse transcription.ofsrc mRNA followed by integration. In the E6 cell line, the provirus unit was arranged as LTR-A gag-src-LTR, indicating a recombination event between the rescued transforming virus and the helper virus. These results suggest that transforming defective viruses containing only the src gene can be rescued from nonpermissive mammalian cells.

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Section: Cells and Virusesmentioning

confidence: 99%

Characterization of Transforming Viruses Rescued from a Hamster Tumour Cell Line Harbouring the v-src Gene Flanked by Long Terminal Repeats

Geryk¹,

Pichrtová²,

Guntaka³

et al. 1986

Journal of General Virology

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“…Therefore, there remains the possibility that contact of permissive chick or duck fibroblasts and RSV-transformed mouse sarcoma cells may cause an induction of viral RNA and transfer of RNA to chick or duck fibroblasts, where replication of RSV may occur. As suggested by SVOBODa (22) and verified by LINDBERG (11, 12) using electron microscopy, intimate contact between the cell membranes of two cell types is of crucial importance for the release of RSV from virogenic mammalian cells.…”

Section: Discussionmentioning

confidence: 90%

“…When RSV-transformed mammalian cells and permissive chick embryo fibroblasts are co-cultivated, infectious RSV is released concomitant with transformation of chick fibroblasts (20) and appearance of heterokaryons, if UVinactivated Sendai virus is used (21,22,23,26). Yet, it is not clear whether fusion of cells is a unconditional event for the activation of RSV.…”

Section: Discussionmentioning

confidence: 98%

Virogenic properties of AKR mouse sarcoma cells induced by Rous sarcoma virus

Kuwata

Okazaki

Spahn³

1973

Archiv f Virusforschung

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SummarySarcomas were induced in AKR mice by Schmidt-Ruppin strain of Rous sarcoma virus (RSV). Sarcoma cells were passaged in vivo and in vitro, and their virogenicities were e~amined. They did not produce any infectious RSV, but when sarcoma cell suspensions were inoculated into wing webs of chickens, sarcomas were induced with high frequencies. Moreover, it was verified by physical and biological methods that clonal line (K3b) of AKR sarcoma cells cultivated in vitro, release murine leukemia virus. These results suggest that RSV and murine leukemia virus genomes coexist in the K3b cells.

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“…Titres of transforming viruses were measured by focus assay in 6-cm polystyrene tissue-culture dishes (Koh-i-noor Hardtmuth, DaleEin, Czechoslovakia) using the schedule described earlier (Svoboda et al, 1968) and expressed as FFU. Individual foci were isolated by cutting the agar over the focus region with a fine scalpel and by transferring the focus with the piece of agar on secondary cultures of BLEF.…”

Section: Virus Testsmentioning

confidence: 99%

Helper virus‐dependent RSV rescue from two lines of hamster cells transformed in vivo by XC RSV

Svoboda

1981

Intl Journal of Cancer

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Newborn hamsters were inoculated with chicken sarcoma tissue produced by XC RSV which had been rescued by transfection from XC cells. Tumours with a latency of up to 20 months appeared in all inoculated hamsters. Three tumour cell lines were successfully established for in vitro growth and cloned. The virus was rescued from the PR RSH-9 cell line by cell fusion with chicken fibroblasts and from the other two lines (PR RSH-12 and PR RSH-14) and clones only if chicken fibroblasts used for fusion had been preinfected with a helper virus. The last two cell lines evidently harboured a functional env gene since the the synthesis of transforming virus started soon after they were found with 16Q cells producing defective BH-RSH(-) lacking the envelope glycoprotein. Produced viruses acquired subgroup C specificity of XC rsv originally used for transformation of these two cell lines. The nature of provirus alterations in PR RSH-12 and PR RSH-14 cells as well as the mechanisms underlying these changes are discussed.

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Rescue of Rous Sarcoma Virus in Mixed Cultures of Virogenic Mammalian and Chicken Cells, Treated and Untreated with Sendai Virus and Detected by Focus Assay

Cited by 39 publications

References 0 publications

Characterization of Transforming Viruses Rescued from a Hamster Tumour Cell Line Harbouring the v-src Gene Flanked by Long Terminal Repeats

Characterization of Transforming Viruses Rescued from a Hamster Tumour Cell Line Harbouring the v-src Gene Flanked by Long Terminal Repeats

Virogenic properties of AKR mouse sarcoma cells induced by Rous sarcoma virus

Helper virus‐dependent RSV rescue from two lines of hamster cells transformed in vivo by XC RSV

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