Virogenic properties of AKR mouse sarcoma cells induced by Rous sarcoma virus

Abstract

SummarySarcomas were induced in AKR mice by Schmidt-Ruppin strain of Rous sarcoma virus (RSV). Sarcoma cells were passaged in vivo and in vitro, and their virogenicities were e~amined. They did not produce any infectious RSV, but when sarcoma cell suspensions were inoculated into wing webs of chickens, sarcomas were induced with high frequencies. Moreover, it was verified by physical and biological methods that clonal line (K3b) of AKR sarcoma cells cultivated in vitro, release murine leukemia virus. These results suggest that RSV and murine leukemia virus genomes coexist in the K3b cells.

Effect of Elevated Temperature on AKR Leukemia Virus Production in AKR Mouse Cells

“…Replication of MLV is known to depend on the cell division (7). Therefore, we at first attempted to isolate a cell line which grows well at 40 C. For our experiments we used K3b cell line which was derived from a sarcoma induced in an AKR mouse by Schmidt-Ruppin strain of Rous sarcoma virus (RSV) (4,5). This cell line was shown to release infectious AKR-MLV but not RSV at 37 C (4,12).…”

“…Therefore, we at first attempted to isolate a cell line which grows well at 40 C. For our experiments we used K3b cell line which was derived from a sarcoma induced in an AKR mouse by Schmidt-Ruppin strain of Rous sarcoma virus (RSV) (4,5). This cell line was shown to release infectious AKR-MLV but not RSV at 37 C (4,12). The cells were cultured in modified Eagle's minimum essential medium supplemented with 10 per cent calf serum.…”

“…This cell line was shown to release infectious AKR-MLV but not RSV at 37 C (4,12). The cells were cultured in modified Eagle's minimum essential medium supplemented with 10 per cent calf serum.…”

“…periments we used K3b cell line which was derived from a sarcoma induced in an AKR mouse by Schmidt-Ruppin strain of Rous sarcoma virus (RSV) (4,5). This cell line was shown to release infectious AKR-MLV but not RSV at 37 C (4,12).…”