Requirement of serum pretreatment for induction of alkaline phosphatase activity with prednisolone, butyrate, dibutyryl cyclic adenosine monophosphate and NaCl in human liver cells continuously cultured in serum-free medium.

Sorimachi, Kenji; Hayashi, Takayuki; Takaoka, T; Niwa, Akira; Yasumura, Yosihiro

doi:10.1247/csf.13.1

Cited by 6 publications

(1 citation statement)

References 11 publications

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“…This observation raises the question of the existence of a possible inhibitor of the uptake of taurine present in the fetal calf serum, MAG cells being more sensitive than TAG cells. It is known that a period of 3 to 5 days is often required to suppress the effects of serum component on cultured cells (see, for examples, Dibner and Insel, 1981;Sorimachi et al, 1988).…”

Section: Discussionmentioning

confidence: 99%

Membrane properties of cultured glial cells obtained mechanically or by enzymatic dissociation: Differential alterations by gangliosides

Bouchon

Petegnief²,

Rebel³

1995

J of Neuroscience Research

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Rat astroglial cells were obtained either mechanically or by enzymatic dissociation and some properties of the primary and secondary cultures were studied. Differences in the ganglioside amount, taurine uptake, membrane fluorescence anisotropy, or their respective modulation by total brain gangliosides confirmed that although the two types of cultures are morphologically similar, they are biochemically distinct.

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Section: Discussionmentioning

confidence: 99%

Membrane properties of cultured glial cells obtained mechanically or by enzymatic dissociation: Differential alterations by gangliosides

Bouchon

Petegnief²,

Rebel³

1995

J of Neuroscience Research

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Studies on the metabolism of metallothionein and alkaline phosphatase of adult rat primary hepatocyte cultures: role of fetal calf serum and agonists of the phosphoinositide cascade

1993

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Adult rat primary hepatocytes maintained in DMEM/F12 (Ham) media were used as a model system for studying the role of fetal calf serum (FCS) and agonists of the phosphoinositide cascade in the metabolism of metallothionein (MT) and alkaline phosphatase (ALP). Experiments were performed both after a 24 h preincubation with FCS and with bovine serum albumin (BSA). Hepatocytes were treated with dexamethasone (DEX), zinc (Zn) and with the agonists of the phosphoinositide cascade A23187, 1,2-dioctanoyl-sn-glycerol (DiC8), 12-O-tetradecanoylphorbol-13-acetate (TPA), angiotensin II (AT), platelet activating factor (PAF), Arg8-vasopressin (VP) and were analyzed for MT and ALP activity in cell homogenates. Cell viability was evaluated by lactate dehydrogenase (LDH) liberation into culture medium, induction of tyrosine aminotransferase (TAT) through DEX and by trypan blue exclusion. Overall, cell viability was improved by the FCS pretreatment and by DEX. Exposure of hepatocytes to the established direct inducers Zn and DEX of MT resulted in a manifold increase in MT, independent of whether the cultures were FCS pretreated or not. The FCS preincubation produced a moderate elevation of ALP activity by stimulating cell viability. However, ALP was unaltered in response to Zn and DEX. None of the experiments conducted with agonists of the phosphoinositide cascade led to an elevation of MT and ALP. Only the incubation of hepatocytes with A23187 resulted in a concentration dependent significant decrease of MT and ALP. This observation was due to a cytotoxic effect of A 23187, displayed by LDH leakage and an increase in the number of cells stained with trypan blue. In conclusion, in primary hepatocyte cultures agonists of the phosphoinositide did not have an effect on the metabolism of MT and ALP. Previous in vivo results indicating alterations of Zn metabolism in liver, therefore seem to be caused by indirect systemic responses.

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Inhibitory effect of dibutyryl cyclic adenosine monophosphate on the induction of alkaline phosphatase in human fetal liver cell line

Sorimachi

Ieiri

Kobayashi

et al. 1989

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research

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Requirement of serum pretreatment for induction of alkaline phosphatase activity with prednisolone, butyrate, dibutyryl cyclic adenosine monophosphate and NaCl in human liver cells continuously cultured in serum-free medium.

Cited by 6 publications

References 11 publications

Membrane properties of cultured glial cells obtained mechanically or by enzymatic dissociation: Differential alterations by gangliosides

Membrane properties of cultured glial cells obtained mechanically or by enzymatic dissociation: Differential alterations by gangliosides

Studies on the metabolism of metallothionein and alkaline phosphatase of adult rat primary hepatocyte cultures: role of fetal calf serum and agonists of the phosphoinositide cascade

Inhibitory effect of dibutyryl cyclic adenosine monophosphate on the induction of alkaline phosphatase in human fetal liver cell line

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