Membrane properties of cultured glial cells obtained mechanically or by enzymatic dissociation: Differential alterations by gangliosides

Abstract

Rat astroglial cells were obtained either mechanically or by enzymatic dissociation and some properties of the primary and secondary cultures were studied. Differences in the ganglioside amount, taurine uptake, membrane fluorescence anisotropy, or their respective modulation by total brain gangliosides confirmed that although the two types of cultures are morphologically similar, they are biochemically distinct.

Neutral glycolipid and ganglioside composition of type-1 and type-2 astrocytes from rat cerebral hemisphere

“…A large proportion of glycosphingolipids are localized not only in neuronal cell bodies (Ando, 1983;Ledeen, 1983;Rosener et al, 1992) and processes, but also in glial cells (Hamburger and Svennerholm, 1971;Poduslo and Norton, 1972;Byrne et al, 1988;Asou and Brunngraber, 1983). A discrepancy has been observed among the results of ganglioside analyses obtained from cultured glial cells Brunngraber, 1983, Asou et al, 1989;Bouchon et al, 1995;Robert et al, 1975;Kim et al, 1986;Mandel et al, 1980;Gottfries et al, 1991). Astrocytes are heterogeneous in nature, with at least two major population types (type-1 and type-2) having been described (Hertz et al, 1982;Fedoroff et al, 1984;Raff et al, 1983a;Miller and Raff, 1984;Lillien et al, 1988).…”

Methods and methodological approaches to studies of isolated neurons of brains from adult animals (Lymnaea stagnalis) in tissue culture

“…If the duration of proteolytic treatment is decreased, significant numbers of glial cells can end up in cultures. Special methods for extracting glial cells have been developed [20].…”