Requirement of polyamines for in-vitro maturation of the mid-binucleate pollen of Nicotiana tabacum

Chibi, Fatiha; Angosto, Trinidad; Garrido, Dolores; Matilla, Ángel J.

doi:10.1016/s0176-1617(11)81251-4

Cited by 26 publications

(19 citation statements)

References 26 publications

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“…Indeed, treatment with SAM might also induce changes in the polyamine profile, since decarboxylation of this compound leads to the aminopropyl donor needed for spermidine and spermine synthesis (Kaur-Sawhney et al 2003). Those polyamines may behave as plant growth regulators, contributing to the control of some of the developmental process that occur in isolated microspores (Chibi et al 1993). It must be said that Chibi and Matilla (1994) have also reported that tobacco microspores treated with ACC did not exhibit any enhancement of the in vitro maturation of mid-nucleate pollen grains.…”

Section: Treatments With Ethephon and Sam Reveal The Inhibitory Effecmentioning

confidence: 99%

Inhibition of ethylene biosynthesis enhances embryogenesis of cultured microspores of Brassica napus

Leroux¹,

Carmoy²,

Giraudet³

et al. 2009

Plant Biotechnol Rep

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The effect of 2,4-dichlorophenoxyacetic acid (2,4-D) applied at high concentrations for a short time was investigated as a novel stress for induction of microspore embryogenesis for the first time. Brassica napus L. cvs. Topas and Hyola 420 were used as model plants for testing this hypothesis. Microspores were subjected to 2,4-D at 4 concentrations (15, 25, 35 and 45 mg/l) for 15-45 min while the classical heat shock was used as the control treatment. Among 2,4-D treatments in Topas, the highest yield of torpedo-stage embryos was achieved at 15 mg/l 2,4-D for 30 min while more normal plantlets were produced when 2,4-D (25 mg/l for 30 min) was applied to the microspores. In Hyola 420 the results showed a lower number of embryos and normal plantlets at all concentrations of 2,4-D. Although Hyola 420 was almost equally embryogenic as Topas after heat shock treatment, large differences between genotypes (concerning embryogenic response) occurred after 2,4-D treatment. However, the mean number of embryos and regenerants was higher in heat shock as compared to 2,4-D induced stress (one magnitude of order). According to the results obtained, 2,4-D can be introduced as a new stress for induction of embryogenesis in microspores similarly like in zygotic and somatic cells. This novel stress is very important for plant species whose microspores are extremely sensitive to classical stresses.

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Section: Treatments With Ethephon and Sam Reveal The Inhibitory Effecmentioning

confidence: 99%

Inhibition of ethylene biosynthesis enhances embryogenesis of cultured microspores of Brassica napus

Leroux¹,

Carmoy²,

Giraudet³

et al. 2009

Plant Biotechnol Rep

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“…The ability to induce nuclear division in cultured pollen tubes is useful in elucidating the mechanism of this unusual haploid mitosis and provides information for successful in vitro pollination and for the isolation of sperm from plant species with bicellular pollen. Although polyamine biosynthesis was required for in vitro maturation of Nicotiana tabacum pollen (Chibi et al, 1993), PA were not involved in the division of the generative nucleus of A. fasciata pollen tubes cultured in vitro. In A. fasciata Arg induced the mitotic division of the generative nucleus, while Orn and DFMO were inhibitory.…”

Section: Discussionmentioning

confidence: 94%

Influence of arginine, ornithine, DFMO and polyamines on division of the generative nucleus in cultured pollen tubes of Aechmea fasciata (Bromeliaceae)

Vervaeke

Stichelbout

Londers

et al. 2005

Plant Cell Tiss Organ Cult

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During in vitro pollen tube growth of Aechmea fasciata the second pollen mitosis (PM II) that produces two sperm cells was influenced by exogenous amino acids. Arginine (Arg) as single amino acid was the limiting factor for the second mitosis of the generative nucleus and thus the formation of sperm cells in cultured pollen tubes of A. fasciata. The involvement of Arg was probably related to protein synthesis. The need for Arg was not related to polyamine (PA) biosynthesis, since PA added to the germination medium were unfavourable for sperm cell production. Both ornithine (Orn) and difluoromethylornithine (DFMO) inhibited the second mitosis in cultured pollen tubes of A. fasciata. The addition of Arg during the first 2 h of pollen germination was necessary to establish the division of the generative nucleus 6 h later.

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“…Flowers sprayed with putrescine increased fruit set in 1-week-old flowers owing to an increase in the period of stigma receptivity and a higher rate of pollen germination, among other effects (Franco-Mora et al 2005b). Moreover, several reports suggest the importance of putrescine in the formation of pollen, pollen germination, and pollen tube growth (Chibi et al 1993;Song et al 2001;Franco-Mora & Tanabe 2008), as well as an association of putrescine with flower development in species such as Citrus (Kushadetal. 1990).…”

Section: Introductionmentioning

confidence: 99%

Putrescine content of Japanese pear (Pyrus pyrifolia) styles increases in response to pollination

Franco-Mora

Tanabe

Tamura

2009

New Zealand Journal of Crop and Horticultural Science

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Although poly amines (Pas) are suggested to be involved in the pollination process, the kinetics of this process in Japanese pear (Pyruspyrifolia) are not currently understood. In this work, the content of putrescine increased (α 0.05) 5 h after in vitro pollination in styles of five different cultivars of Japanese pear. The evolution of spermidine and spermine within the cultivars used was not consistent; whereas spermidine contents decreased, increased, or did not have statistically significant changes (α 0.05), spermine content either decreased or increased. Moreover, the regulation of the ethylene and putrescine responses to pollination seemed to be linked to the cultivar's gynoecious genotype, and was influenced by the pollen source. These results agree with the idea that putrescine increases in the styles during the first few hours after pollination in this species.

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Requirement of polyamines for in-vitro maturation of the mid-binucleate pollen of Nicotiana tabacum

Cited by 26 publications

References 26 publications

Inhibition of ethylene biosynthesis enhances embryogenesis of cultured microspores of Brassica napus

Inhibition of ethylene biosynthesis enhances embryogenesis of cultured microspores of Brassica napus

Influence of arginine, ornithine, DFMO and polyamines on division of the generative nucleus in cultured pollen tubes of Aechmea fasciata (Bromeliaceae)

Putrescine content of Japanese pear (Pyrus pyrifolia) styles increases in response to pollination

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