Requirement of Deoxyribonucleic Acid Synthesis for Microcycle Sporulation in
            <i>Bacillus megaterium</i>

Mychajlonka, Myron; Slepecky, Ralph A.

doi:10.1128/jb.120.3.1331-1338.1974

Cited by 18 publications

(5 citation statements)

References 30 publications

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“…Instead, the partially germinated cysts reinitiated encystment to yield two or more normal appearing cysts encased in an intine and exine. The phenomenon they described is completely analogous to microcycle sporulation, which has been extensively studied in B. megaterium (50,107,108,170). Cylindrical structures can be seen in cells of A. vinelandii which have been grown in N-free media, fixed with KMnO, (180) or OsO (116), and examined by thin-section electron microscopy.…”

Section: Figmentioning

confidence: 95%

Encystment and germination in Azotobacter vinelandii.

Sadoff¹

1975

Bacteriological Reviews

121

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Section: Figmentioning

confidence: 95%

Encystment and germination in Azotobacter vinelandii.

Sadoff¹

1975

Bacteriological Reviews

121

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“…The authors speculated that glutamine synthetase would be the receptor for this effector. Relationship to DNA Replication and the Cell Division Cycle There is now strong evidence that initiation of sporulation is tied to the cell cycle (60, 61,254), and can only occur while DNA is being replicated (59,73,198,200,217). When chromosome replication of a thymidine-requiring mutant was prevented by thymidine starvation, then this prevented the production, in a sporulation medium, of serine protease and all the subsequent sporulation events tested (56).…”

Section: Downloaded Frommentioning

confidence: 99%

Genetic aspects of bacterial endospore formation.

Piggot¹,

Coote²

1976

Bacteriological Reviews

405

283

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95 cysA14 60 ery-1 25 purA 49 metC3; 21 ura-1 c 42 ura-26 s 83 ura-1; 5 metC3 ' 54 lys-l' 44 trpC2 35 trpC2 33 Iys-1; 16 trpC2 56 lys-1 25 phe-1; 10 ilvCl RI = 0.1 12 hisAI RI s 0.2 47 ura-1; 1 metC3 12 ura-26 16 ura-1; 49 metC3 49 52 148 307 24 phe-12; 1 lys-l 49 B 49 49, 143, 334 49 244 49 a Linkage with vegetative markers is expressed as % cotransfer. Linkage between spo mutations is expressed as the recombination index (RI [170]). b A, J.A. Hoch, personal communication; B, M. Young, personal communication; C, P.J. Piggot, unpublished observations. I Assignment to locus is not certain, see text. d If 94U (reference 148) is the same as 94 (reference 257), then this has the same phenotype as spoIVC mutants described by Coote (49, 50), and the mutations should be placed in the spoIVC locus.

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“…The relationship between sporulation and cell cycle has also been studied by using microcycle sporogenesis (i.e., production of spores from spores without intervening cell division [21,22]). Microcycle sporogenesis can be obtained in Bacillus megaterium by transferring germinating spores into an SM.…”

mentioning

confidence: 99%

Chromosome Replication in Sporulating Cells of Bacillus subtilis

Sargent

1980

J Bacteriol

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A method of specifically labeling the chromosomal terminus of Bacillus subtilis is described. When sporulating cultures were pulse-labeled with [ 3 H]thymidine and then treated with 6-( p -hydroxyphenylazo)uracil, a drug which inhibits deoxyribonucleic acid (DNA) synthesis rapidly and completely, the only labeled spores formed were those that had completed replication during the pulse period. DNA-mediated transformation was used to show that the DNA of spores formed in the presence of 6-( p -hydroxyphenylazo)uracil had the same ratio of origin to terminus markers as DNA from untreated spores. Furthermore, spores formed in the presence of 6-( p -hydroxyphenylazo)uracil had the same DNA content as untreated spores. These two observations indicated that spores formed in the presence of 6-(hydroxyphenylazo)uracil contained completed chromosomes. The rate of termination of chromosomes destined to be packaged into spores was determined by this method, using the Sterlini-Mandelstam replacement system and a single medium exhaustion system for inducing sporulation. With both systems the rate of termination reached a broad peak 2 h after the start of sporogenesis. This was measured from the time of resuspension by using the replacement system and from the point where exponential growth ceased in the exhaustion system. The amount of spore DNA synthesized in the Sterlini-Mandelstam sporulation-inducing medium was very close to one-half the amount of the DNA present in mature spores. This suggests that chromosomes destined to be packaged into spores were replicated from close to the origin and possibly initiated in the sporulation-inducing medium. A method was devised for estimating the time taken to complete replication of the chromosomes destined to be packaged into spores. This was probably no more than 50 min. Whereas starvation must have occurred almost simultaneously in most cells in the population, the chromosome replication that was essential for sporogenesis was distributed over a wide time span. Thus, in some cells, replication started within 10 min of the nutritional step-down, but the peak rate was not reached for 1 h; thereafter replication continued at a substantial rate.

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Requirement of Deoxyribonucleic Acid Synthesis for Microcycle Sporulation in Bacillus megaterium

Cited by 18 publications

References 30 publications

Encystment and germination in Azotobacter vinelandii.

Encystment and germination in Azotobacter vinelandii.

Genetic aspects of bacterial endospore formation.

Chromosome Replication in Sporulating Cells of Bacillus subtilis

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