Genetic aspects of bacterial endospore formation.

Abstract

95 cysA14 60 ery-1 25 purA 49 metC3; 21 ura-1 c 42 ura-26 s 83 ura-1; 5 metC3 ' 54 lys-l' 44 trpC2 35 trpC2 33 Iys-1; 16 trpC2 56 lys-1 25 phe-1; 10 ilvCl RI = 0.1 12 hisAI RI s 0.2 47 ura-1; 1 metC3 12 ura-26 16 ura-1; 49 metC3 49 52 148 307 24 phe-12; 1 lys-l 49 B 49 49, 143, 334 49 244 49 a Linkage with vegetative markers is expressed as % cotransfer. Linkage between spo mutations is expressed as the recombination index (RI [170]). b A, J.A. Hoch, personal communication; B, M. Young, personal communication; C, P.J. Piggot, unpublished observations. I Assignment to locus is not certain, see text. d If 94U (reference 148) is the same as 94 (reference 257), then this has the same phenotype as spoIVC mutants described by Coote (49, 50), and the mutations should be placed in the spoIVC locus.

Distinct and essential morphogenic functions for wall- and lipo-teichoic acids in Bacillus subtilis

“…However, GFP-YqgS still localized to polar septa in a spoIIE deletion background ( Figure 5C). Indeed, fluorescence was often clearly visible at polar septa near both cell poles; a phenotype characteristic of spoIIE mutants (Piggot and Coote, 1976;Illing and Errington, 1991). These results support the idea that LTA synthesis is particularly important for cell division.…”

“…s E (Glenn and Mandelstam, 1971;Piggot and Coote, 1976;Stragier et al, 1984;Illing and Errington, 1991) was also blocked by the ltaS yqgS double deletion ( Figure 4C). This effect was not due to a failure in translocation of the prespore chromosome (Wu and Errington, 1994), based on (i) DAPI staining (data not shown) and (ii) the fact that the spoIIQ-lacZ fusion was located at amyE, which is captured in the prespore compartment even when chromosome translocation fails (Wu and Errington, 1994).…”

A developmental checkpoint couples the initiation of sporulation to DNA replication in Bacillus subtilis.

“…The smaller cell, the forespore, develops into the spore within the mother cell. Proper development of the spore requires gene expression and an intact chromosome in each cell type (Piggot and Coote, 1976;Losick and Stragier, 1992).…”

Protein synthesis during outgrowth of Bacillus subtilis spores. A two-dimensional gel electrophoresis study

“…genes, whose expression is switched off during sporulation, might be activated by outgrowthspecific events. Possibly their expression depends on the previous activation of regulatory genes according to a dependent sequence hypothesis as postulated for sporulating cells [18]. The existence of special outgrowth genes can be deduced from genetic analysis of Galizzi and coworkers.…”