Requirement of a bone morphogenetic protein for the maintenance and stimulation of osteoblast differentiation

Martinović, Snježana; Borovečki, Fran; Miljavac, Vera; Kisic, Veronika; Matičić, Dražen; Francetić, Igor; Vukičević, Slobodan

doi:10.1679/aohc.69.23

Cited by 36 publications

(33 citation statements)

References 62 publications

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“…In fact, the expression of both the BMP-2 mRNA and protein were enhanced greatly after three days of culture in this study, which implies the induction of osteoblast differentiation. However, the decrease after seven days of culture indicates that a negative feedback mechanism might act against its overexpression after three days of culture 23) . Integrins are heterodimers consisting of subunits comprising α and β chains, and exist in the cell membrane.…”

Section: Discussionmentioning

confidence: 98%

Early gene and protein expression associated with osteoblast differentiation in response to fish collagen peptides powder

et al. 2013

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This study was designed to investigate the biological effects of fish collagen peptide (FCP) on human osteoblasts. Human osteoblasts were treated with 0.1% FCP, which was the optimal concentration confirmed by the increase in alkaline phosphatase activity. After one, three, five and seven days of culture, the number of FCP-treated cells increased significantly compared with untreated cells. In a real-time PCR analysis, the expression of osteocalcin, osteopontin, BMP-2 and integrin β3 mRNAs in FCP-treated cells showed increases compared with untreated cells after three days of culture. After seven days of culture, the expression levels of osteopontin and integrin β3 were still higher in the FCP-treated cells than in untreated cells. The production of osteocalcin, osteopontin and integrin β3 proteins in FCP-treated cells also showed increases after seven days of culture. Furthermore, FCP accelerated matrix mineralization in the cultures. The present study indicates the potential utility of FCP as a biomaterial.

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Section: Discussionmentioning

confidence: 98%

Early gene and protein expression associated with osteoblast differentiation in response to fish collagen peptides powder

et al. 2013

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“…MC3T3-E1 cells synthesize BMP-4, which induces osteoblast differentiation (Martinovic et al, 2006;Wang et al, 2006). We next examined the effect of an antibody against BMP-4 on the mineralization of MC3T3-E1 cells induced by CS-E.…”

Section: Cs-e Enhanced the Differentiation Of Mc3t3-e1 Cells By Intermentioning

confidence: 99%

Oversulfated chondroitin sulfate‐E binds to BMP‐4 and enhances osteoblast differentiation

Miyazaki

Miyauchi²,

Tawada³

et al. 2008

Journal Cellular Physiology

109

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Small leucine-rich proteoglycans, such as biglycan, and their side chain sulfated glycosaminoglycans (GAGs), have been suggested to be involved in bone formation and mineralization processes. The present study was designed to investigate whether chondroitin sulfate (CS), one of the GAG, and its oversulfated structures coupled with bone morphogenetic protein-4 (BMP-4) alter the differentiation and subsequent mineralization of MC3T3-E1 osteoblastic cells. CS-E, one of the oversulfated CS structure, enhanced cell growth, alkaline phosphatase (ALP) activity, collagen deposition, and mineralization whereas heparin enhanced only ALP activity and mineralization. As well as CS-E, CS-H, and CPS also enhanced the mineralization of the cells. CS-E enhanced the mineralization of the cells by interacting with protein in the conditioned medium. CS-E induced mineralization was significantly inhibited by an antibody against BMP-4. The addition of exogenous BMP-4 further increased the capacity of CS-E to enhance mineralization. Fluorescence correlation spectroscopy method using fluoresceinamine-labeled GAG revealed that the oversulfated GAGs have a high affinity for BMP-4. The disaccharide analysis of the cells indicated that MC3T3-E1 cells are capable of producing oversulfated structures of CS by themselves. The lack of CS from the cells after chondroitinase treatment resulted in the inhibition of mineralization. These results in the present study indicate that oversulfated CS, which possesses 4,6-disulfates in N-acetyl-galactosamine, binds to BMP-4 and promotes osteoblast differentiation and subsequent mineralization.

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“…They were discovered and named based on their osteoinductive ability. Today we know that BMPs have an essential role in embryonic development and tissue differentiation, regulation of cell survival, and apoptosis, as well as in the organogenesis of skeletal and nonskeletal tissues (14,45,60,67,68,69,74).…”

mentioning

confidence: 99%

BMP signaling in rats with TNBS-induced colitis following BMP7 therapy

Marić¹,

Kučić

Wensveen

et al. 2012

American Journal of Physiology-Gastrointestinal and Liver Physi

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yond stimulating bone formation, bone morphogenetic proteins (BMPs) are important in development, inflammation, and malignancy of the gut. We have previously shown that BMP7 has a regenerative, anti-inflammatory, and antiproliferative effect on experimental inflammatory bowel disease (IBD) in rats. To further investigate the BMP signaling pathway we monitored the effect of BMP7 therapy on the BMP signaling components in the rat colon during different stages of experimentally induced colitis by 2,4,6-trinitrobenzene sulfonic acid (TNBS). The results showed a significantly decreased BMP7 expression in the acute phase, followed by a significantly increased BMP2 and decreased BMP6 expression during the chronic phase of colitis. BMP7 therapy influenced the expression of several BMPs with the most prominent effect on downregulation of BMP2 and upregulation of BMP4 in the chronic phase of colitis. Importantly, connective tissue growth factor and noggin expression were elevated in the acute stage and significantly decreased upon BMP7 therapy. BMP receptor I expression was unchanged, whereas BMP receptor II was decreased at day 2 and increased at days 14 and 30 of TNBS inflammation. However, an opposite pattern of expression following BMP7 therapy has been observed. BMP7 increased the expression of BR-Smad including Smad3 and Smad4. Inhibitory Smads were increased in colitis and significantly decreased following BMP7 therapy at later stages of the disease. We suggest that BMP signaling was altered during TNBS-induced colitis and was recovered with BMP7 administration, suggesting that IBD is a reversible process.

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Requirement of a bone morphogenetic protein for the maintenance and stimulation of osteoblast differentiation

Cited by 36 publications

References 62 publications

Early gene and protein expression associated with osteoblast differentiation in response to fish collagen peptides powder

Early gene and protein expression associated with osteoblast differentiation in response to fish collagen peptides powder

Oversulfated chondroitin sulfate‐E binds to BMP‐4 and enhances osteoblast differentiation

BMP signaling in rats with TNBS-induced colitis following BMP7 therapy

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