Reprogramming to pluripotency is an ancient trait of vertebrate Oct4 and Pou2 proteins

Tàpia, Natalia; Reinhardt, Peter; Duemmler, Annett; Wu, Guangming; Araúzo-Bravo, Marcos J.; Esch, Daniel; Greber, Boris; Cojocaru, Vlad; Rascon, Cynthia Alexander; Kato, Masaya; Kump, Kevin; Voss, Randal; Tanaka, Elly M.; Schöler, Hans R.

doi:10.1038/ncomms2229

Cited by 69 publications

(79 citation statements)

References 41 publications

(54 reference statements)

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“…In the new Pou2/Pou5f1 hypothesis, Pou5f1 would be extinct in the lesser spotted dogfish, as it is assumed to be in the elephant fish lineage but retained in some rays and sharks. Both Pou2 and Pou5f1 (Oct4) show the feature of induction of pluripotency (Tapia et al 2012). In testis, Pou2/Pou5f1 is an important marker of pluripotency for the spermatogonial lineage and was involved in the stem cell fate maintenance (Tenenhaus Dann et al 2008).…”

Section: Pou2/pou5f1 and Gfra1 Are Highly Conserved Sscs Markersmentioning

confidence: 99%

“…Only three recent studies of teleosts have described the expression pattern of the classical molecular markers we chose in this study (SanchezSanchez et al 2010, Ozaki et al 2011, Lacerda et al 2013. Pou2, known for its role in the induction of pluripotency (Tapia et al 2012), and Gfra1, involved in SSC self-renewal in response to GDNF (Naughton et al 2005), seem well conserved as markers of undifferentiated spermatogonia from Osteichthyes (mammals and teleosts) to Chondrichthyes. Plzf, responsible for the maintenance of their undifferentiated condition (Buaas et al 2004, Costoya et al 2004, always shows an expression in undifferentiated spermatogonia extending to differentiating spermatogonia in zebrafish (Ozaki et al 2011) and even further in dogfish.…”

Section: Spermatogonial Markers Seem Well Conserved Among Vertebratesmentioning

confidence: 99%

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Characterization of spermatogonial markers in the mature testis of the dogfish (Scyliorhinus canicula L.)

Bosseboeuf¹,

Gautier²,

Auvray³

et al. 2014

Reproduction

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In dogfish, spermatogenesis progresses from a restricted germinative zone, which lines the dorsal testicular vessel. Single spermatogonia (A s ), including the spermatogonial stem cells (SSCs), produce successively paired (A p ), undifferentiated (A u4 to A u512 ), and differentiated (A d1 to A d8 ) spermatogonia and preleptotene (PL) spermatocytes through 13 mitoses. Dogfish spermatogonial subpopulations present classical morphological characteristics but cannot be distinguished on the basis of molecular markers. This characterization has been initiated in mammals despite the difficulty to separate each spermatogonial subpopulation. For instance, both glial cell-derived neurotrophic factor family receptor alpha 1 (GFRa1) and promyelocytic leukemia zinc finger protein (PLZF) are markers of undifferentiated spermatogonia, whereas receptor tyrosine kinase C-kit is a marker of differentiated spermatogonia. The aim of this study is to characterize spermatogonial markers and to differentiate several spermatogonial subpopulations. Dogfish cDNA sequences have been identified and validated by phylogenetic analyses for gfra1, plzf, pou2, as well as for high-mobility group box proteins 2 and 3 (hmgb2 and 3) and for mini-chromosome maintenance protein 6 (mcm6). We have used the anatomical advantage of the polarized dogfish testis to analyze the expression of those markers by RT-PCR and in situ hybridization. gfra1, pou2, and plzf have been detected in the testicular germinative zone, suggesting that spermatogonial markers are relatively well conserved among vertebrates but with a less restricted expression for plzf. Moreover, hmgb3 and mcm6 have been identified as new markers of differentiated spermatogonia. Finally, this first molecular characterization of spermatogonial subpopulations in a chondrichthyan model will be useful for further studies on the SSC niche evolution.

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Section: Pou2/pou5f1 and Gfra1 Are Highly Conserved Sscs Markersmentioning

confidence: 99%

Section: Spermatogonial Markers Seem Well Conserved Among Vertebratesmentioning

confidence: 99%

Characterization of spermatogonial markers in the mature testis of the dogfish (Scyliorhinus canicula L.)

Bosseboeuf¹,

Gautier²,

Auvray³

et al. 2014

Reproduction

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“…The gene structure of porcine OCT4B and OCT4B1 are conserved OCT4A sequences and domains among species were highly conserved and the function of the gene has been suggested to be an ancient trait of vertebrates (Tapia et al 2012). The structure of variant mRNA is similar between human and mice, including POU-and CTD-coding exons with differential NTD-coding exon compared with the A form.…”

Section: Discussionmentioning

confidence: 99%

Identification and differential expression patterns of porcine OCT4 variants

Hwang

Lee

et al. 2015

Reproduction

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OCT4 encoded by POU5F1 has a crucial role of maintaining pluripotency in embryonic stem cells during early embryonic development and several OCT4 variants have been identified in mouse and human studies. The objective of this study was to identify different variants of OCT4 and analyze their expression patterns in preimplantation porcine embryos and various tissues. In this study, we showed that POU5F1 transcribes its three variants, namely OCT4A, OCT4B, and OCT4B1. The OCT4B transcript consists of exons identical to the major form of the OCT4 variant, OCT4A, with a differential N-terminal domain-coding exon. The structure of OCT4B1 mRNA was the same as that of OCT4B mRNA, but harbored a cryptic exon. Based on these findings, the transcription levels were investigated and found that OCT4B and OCT4B1 made up w20% among the variants in the embryonic stage and this indicates that OCT4A mRNA is dominantly expressed during preimplantation embryo development. In addition, OCT4B mRNA was detected in all tissues examined, while OCT4A and OCT4B1 were detected only in testis but not in other tissues examined. OCT4B1 showed inversely correlated expression with SOX2 and NANOG expression. OCT4A protein was specifically localized to the nuclei, whereas OCT4B was mainly localized to the cytoplasm of the porcine embryos at the blastocyst stage. The findings of this study reveal that the porcine OCT4 gene can potentially encode three variants (OCT4A, OCT4B, and OCT4B1), and they are differentially expressed and would have roles dissimilar between each other in preimplantation embryos and various adult tissues.

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“…Among vertebrates, zebrafish Pou5f1 might be somewhat of an outlier in this regard. Whereas zebrafish Pou5f1 cannot reprogram mammalian fibroblasts to pluripotency, medaka and axotoxl Pou5f1 can (Tapia et al, 2012). These findings suggest that this function is ancestral and has been lost in zebrafish.…”

Section: Box 2 Zebrafish Oct Proteinsmentioning

confidence: 95%

Oct transcription factors in development and stem cells: insights and mechanisms

Tantin

2013

Development

113

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The POU domain family of transcription factors regulates developmental processes ranging from specification of the early embryo to terminal differentiation. About half of these factors display substantial affinity for an 8 bp DNA site termed the octamer motif, and are hence known as Oct proteins. Oct4 (Pou5f1) is a well-known Oct factor, but there are other Oct proteins with varied and essential roles in development. This Primer outlines our current understanding of Oct proteins and the regulatory mechanisms that govern their role in developmental processes and concludes with the assertion that more investigation into their developmental functions is needed.

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Reprogramming to pluripotency is an ancient trait of vertebrate Oct4 and Pou2 proteins

Cited by 69 publications

References 41 publications

Characterization of spermatogonial markers in the mature testis of the dogfish (Scyliorhinus canicula L.)

Characterization of spermatogonial markers in the mature testis of the dogfish (Scyliorhinus canicula L.)

Identification and differential expression patterns of porcine OCT4 variants

Oct transcription factors in development and stem cells: insights and mechanisms

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