Oct transcription factors in development and stem cells: insights and mechanisms

Tantin, Dean

doi:10.1242/dev.095927

Cited by 113 publications

(95 citation statements)

References 124 publications

(126 reference statements)

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“…Oct proteins are a group of developmentally important POU domain-containing transcription factors that recognize a specific 8 nt DNA sequence (octamer) (Tantin, 2013). The vertebrate Oct3/4 is a core transcription factor that activates protein-coding genes and non-coding RNAs required for pluripotency (Shi and Jin, 2010).…”

Section: Resultsmentioning

confidence: 99%

“…The vertebrate Oct3/4 is a core transcription factor that activates protein-coding genes and non-coding RNAs required for pluripotency (Shi and Jin, 2010). In mammalian stem cells, Oct3/4 is co-expressed together with Oct1, a related transcription factor that is also known to be involved in maintenance of the stem cell phenotype (Maddox et al, 2012; Tantin, 2013). For example, Oct1 is required for radial glia formation in the CNS and is also strongly expressed in Lgr5-positive intestinal stem cells (Kiyota et al, 2008; Maddox et al, 2012).…”

Section: Resultsmentioning

confidence: 99%

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Expression of pluripotency factors in echinoderm regeneration

2014

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Cell dedifferentiation is an integral component of post-traumatic regeneration in echinoderms. Since dedifferentiated cells become multipotent, we asked if this spontaneous broadening of developmental potential is associated with the action of the same pluripotency factors (known as Yamanaka factors) that were used to induce pluripotency in specialized mammalian cells. In this study, we investigate expression of orthologs of the four Yamanaka factors in regeneration of two different organs, the radial nerve cord and the digestive tube, in the sea cucumber Holothuria glaberrima. All four pluripotency factors are expressed in uninjured animals, although their expression domains do not always overlap. In regeneration, the expression levels of the four genes were not regulated in a coordinated way, but, instead, showed different dynamics for individual genes and also were different between the radial nerve and the gut. SoxB1, the ortholog of the mammalian Sox2, was drastically downregulated in the regenerating intestine, suggesting that this factor is not required for dedifferentiation/regeneration in this organ. On the other hand, during the early post-injury stage, Myc, the sea cucumber ortholog of c-Myc, was significantly upregulated in both the intestine and the radial nerve cord, and is, therefore, hypothesized to play a central role in dedifferentiation/regeneration of various tissue types.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Expression of pluripotency factors in echinoderm regeneration

2014

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“…ct4/Pou5f1 is an indispensable component of the regulatory circuitry controlling the establishment and maintenance of pluripotent, undifferentiated inner cell mass and epiblast cells, as well as primordial germ cells, germ line progenitor cells, and cultured embryonic stem cells (ESCs) (1)(2)(3). Its loss accompanies ESC differentiation and loss of pluripotency (4).…”

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confidence: 99%

Pluripotency Transcription Factor Oct4 Mediates Stepwise Nucleosome Demethylation and Depletion

Shakya

Callister

Goren

et al. 2015

Molecular and Cellular Biology

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Section: Introductionmentioning

confidence: 99%

“…Pluripotency transcription factors in mammalian stem cells are regulated by key genes, such as OCT4, SOX2 and NANOG. OCT4 is a member of the octamer-binding subgroup of the Pit-Oct-Unc transcription factor family (16,17).Due to a lack of information on the establishment of planarian primary neoblast cultures, particularly of Girardia tigrina, the present study proposed a methodology for cell culture establishment and characterization of neoblasts. …”

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confidence: 99%

Method of isolation and characterization of Girardia tigrina stem cells

2014

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Abstract. Tissue regeneration is widely studied due to its importance for understanding the biology of stem cells, aiming at their application in medicine for therapeutic and various other purposes. The establishment of experimental models is necessary, as certain invertebrates and vertebrates have different regeneration abilities depending on their taxon position on the evolutionary scale. Planarians are an efficacious in vivo model for stem cell biology, but the correlation between planarian cellular and molecular neoblast pluripotency mechanisms and those of mammalian stem cells is unknown. The present study had the following objectives: i) Establish Girardia tigrina cell culture, ii) determine the time required for complete cell disintegration and iii) obtain neoblasts by cell subdivision. Twenty-four specimens were deprived of food for seven days. After this time, disintegration was performed by incubation protected at three temperatures for 48 h in an antibiotic, antimycotic and trypsin solution, after which the suspension was homogenized and centrifuged. Histopaque ® 1077 was used for cell separation and interphases were collected and monitored by optical and fluorescence microscopy. Optical microscopy analysis informed the nucleus-to-cytoplasm ratio, cell morphology and cell size. Under fluorescence microscopy, interphase 1 (I1) was subdivided into two groups and neoblasts were marked for characterization; one group was stained with 4',6-diamidino-2-phenylindole and the other was immunolabeled with octamer-binding transcription factor 4 (OCT4) and isolated and observed after 10 days of cultivation. Neoblasts predominated in I1 with a small amount of other cell types. In conclusion, sample disintegration with a trypsin and antibiotic solution was effective at 18˚C and Iscove's modified Dulbecco's medium supplemented with fetal bovine serum was adequate for the establishment of primary cell cultures after 48-h incubation and centrifugation. Antibody anti-OCT4 was used for the characterization of stem cells and was successfully labeled with concentrated neoblasts on interphase 1. IntroductionRegeneration is a complex event that occurs in several vertebrates and invertebrates (1). For regeneration to occur, one of the earliest signaling events following a lesion is the production of cells that are capable of rebuilding lost structures. The way that these events occur and the types of cells involved differ between animal groups (2).The choice of planarians as a regeneration study model is based on their plasticity, regeneration ability, rapid response and adult stem cell reservoir (3). Cell and molecular characterization of planarian stem cells has been achieved with various tools, such as inhibition of the function of genes involved in regeneration via RNA interference, western blotting, in situ hybridization and cell isolation by flow cytometry (4-8). These tools are extremely important alternatives for understanding the mechanisms associated with the maintenance and role of stem cells in vivo (9).Stem cell...

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Oct transcription factors in development and stem cells: insights and mechanisms

Cited by 113 publications

References 124 publications

Expression of pluripotency factors in echinoderm regeneration

Expression of pluripotency factors in echinoderm regeneration

Pluripotency Transcription Factor Oct4 Mediates Stepwise Nucleosome Demethylation and Depletion

Method of isolation and characterization of Girardia tigrina stem cells

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