Reproductive toxicity of 2-methoxyethanol applied dermally to occluded and nonoccluded sites in male rats

Feuston, Maureen H.; Bodnár, Károly; Kerstetter, S.L.; Grink, C.P.; Belcak, M.J.; Singer, E.J.

doi:10.1016/0041-008x(89)90098-7

Cited by 33 publications

(12 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The film was air dried and then observed under a microscope for changes in sperm morphology according to the method of Feustan et al [27]. The following sperm abnormalities were counted in two separate fields in each of the sperm samples described above: absence of head, absence of tail, tail bending, tail coiling, mid-piece curving and mid-piece bending.…”

Section: Methodsmentioning

confidence: 99%

Resveratrol Reverses Cadmium Chloride-induced Testicular Damage and Subfertility by Downregulating p53 and Bax and Upregulating Gonadotropins and Bcl-2 gene Expression

Eleawa

Alkhateeb

Al‐Hashem

et al. 2014

Journal of Reproduction and Development

100

View full text Add to dashboard Cite

This study was performed to investigate the protective and therapeutic effects of resveratrol (RES) against CdCl2-induced toxicity in rat testes. Seven experimental groups of adult male rats were formulated as follows: A) controls+NS, B) control+vehicle (saline solution of hydroxypropyl cyclodextrin), C) RES treated, D) CdCl2+NS, E) CdCl2+vehicle, F) RES followed by CdCl2 and M) CdCl2 followed by RES. At the end of the protocol, serum levels of FSH, LH and testosterone were measured in all groups, and testicular levels of TBARS and superoxide dismutase (SOD) activity were measured. Epididymal semen analysis was performed, and testicular expression of Bcl-2, p53 and Bax was assessed by RT-PCR. Also, histopathological changes of the testes were examined microscopically. Administration of RES before or after cadmium chloride in rats improved semen parameters including count, motility, daily sperm production and morphology, increased serum concentrations of gonadotropins and testosterone, decreased testicular lipid peroxidation and increased SOD activity. RES not only attenuated cadmium chloride-induced testicular histopathology but was also able to protect against the onset of cadmium chloride testicular toxicity. Cadmium chloride downregulated the anti-apoptotic gene Bcl2 and upregulated the expression of pro-apoptotic genes p53 and Bax. Resveratrol protected against and partially reversed cadmium chloride testicular toxicity via upregulation of Bcl2 and downregulation of p53 and Bax gene expression. The antioxidant activity of RES protects against cadmium chloride testicular toxicity and partially reverses its effect via upregulation of BCl2 and downregulation of p53 and Bax expression.

show abstract

Section: Methodsmentioning

confidence: 99%

Resveratrol Reverses Cadmium Chloride-induced Testicular Damage and Subfertility by Downregulating p53 and Bax and Upregulating Gonadotropins and Bcl-2 gene Expression

Eleawa

Alkhateeb

Al‐Hashem

et al. 2014

Journal of Reproduction and Development

100

View full text Add to dashboard Cite

show abstract

“…The concentration of the hormones T3 (total triiodothyronine), T4 (total thyroxine), and TSH (thyroid stimulating hormone) was determined using commercially available radioimmunoassay (RIA) test kits and a Gamma Counter (LB 2111; Berthold, Germany). Sperm morphology and sperm head count were evaluated from the animals of the control and high-dose groups only according to the methods of Feuston et al (1989). Two-hundred eosin stained spermatozoa per animal were examined for abnormal heads and tails.…”

Section: Clinical Pathologymentioning

confidence: 99%

Detection of endocrine-modulating effects of the antithyroid acting drug 6-propyl-2-thiouracil in rats, based on the “Enhanced OECD Test Guideline 407”

Mellert¹,

Deckardt²,

Walter³

et al. 2003

Regulatory Toxicology and Pharmacology

View full text Add to dashboard Cite

“…erefore, sperm concentration was expressed in terms of sperm X 10 6 /ml. Two samples from each epididymis were examined, and the average counts were scheduled [12,[15][16][17].…”

Section: Sperm Countsmentioning

confidence: 99%

Gestational Exposure to Synthetic Steroid Hormones Impaired Sperm Quantity and Quality in Wistar Rats

Ahmed

Magzoub

Al-Ayed

et al. 2020

International Journal of Endocrinology

View full text Add to dashboard Cite

This study was designed to investigate the effect of prenatal exposure to synthetic sex steroid on sperm quantity and quality, relative testicular and epididymal weights, and reproductive hormones level in adult Wistar rats. Forty male Wistar rats were divided into two groups: a test group (n = 20) that included mature rats that were born to dams exposed to gestational treatment with hydroxyprogesterone and a control group (n = 20) that included mature rats born to untreated dams. Compared to the control group, the test group showed a significant reduction in the sperm count, viability and motility, relative testicular and epididymal weights together with increased abnormal spermatozoa (p<0.001). The reproductive hormonal assay revealed significantly lower serum testosterone and higher levels of FSH and LH among the test groups compared to the control (p<0.05 for all). Prenatal exposure to synthetic progesterone negatively affected sperm production and function, relative testicular and epididymal weights, and reproductive hormone levels.

show abstract

Reproductive toxicity of 2-methoxyethanol applied dermally to occluded and nonoccluded sites in male rats

Cited by 33 publications

References 7 publications

Resveratrol Reverses Cadmium Chloride-induced Testicular Damage and Subfertility by Downregulating p53 and Bax and Upregulating Gonadotropins and Bcl-2 gene Expression

Resveratrol Reverses Cadmium Chloride-induced Testicular Damage and Subfertility by Downregulating p53 and Bax and Upregulating Gonadotropins and Bcl-2 gene Expression

Detection of endocrine-modulating effects of the antithyroid acting drug 6-propyl-2-thiouracil in rats, based on the “Enhanced OECD Test Guideline 407”

Gestational Exposure to Synthetic Steroid Hormones Impaired Sperm Quantity and Quality in Wistar Rats

Contact Info

Product

Resources

About