Reproductive potency of methanolic bi-herbal (Zingiber officinale and Chrysophyllum albidum) extract on masculine wistar rats

Abstract

The study is aimed at evaluating the fertility potential of bi-herbal methanol extract on male Wistar rats. This research finding based it fact of fertility in male Wistar rats to investigate through the phytochemicals, antioxidant assay, hormonal indexes, lipid profile, body/organ weight changes and histopathological study across the graded doses (25, 50, 100 mg/kg) of the treated groups of bi-herbal (Zingiber officinale and Chrysophyllum albidum)) methanol extract using standard procedure. Results from the phytochemical screening elicited the essential constituents (alkaloids, flavonoids phenol, tannins and saponnins). The antioxidant assay showed broad scavenging property against free radicals, which possibly is associated with one of its mechanisms of action. An increase in the level of significant (p > 0.05) in hormonal indexes (testosterone, progesterone, follicle stimulating hormone and luteinizing hormone) and slight increase in the level of lipid profile (cholesterol, triglyceride, HDL LDL and VLDL) activities among the treatment groups when compared with the control. There absent significant change (p > 0.05) in the body and testes mass ratio. No pronounce histopathological variation on the testes state in comparative to the control. The dependent from the bi-herbal extract with extreme exhibition of fertility potential as its therapeutic effect

Effect of Dosages and Temperatures on Simalungun Red Ginger (<I>Zingiber officinale</I> var. Rubrum) Ethanol Extract Dipping on Masculinization of Zebra Fish (<I>Danio rerio</I>) as a Model Fish

“…The extraction method according to Ghasemzadeh et al (2016) and Benjamin et al (2020) was as follow; RG was rinsed with clean water and dried under shade at room temperature, the dried plant samples were ground into a fine powder. Two kilograms of wet RG only produced 250 grams of dry RG sample, therefore, the sample needed to be dissolved in 1 L n-hexane, 1 L 96% ethanol, and 1 L methanol according to Benjamin et al (2020) with a ratio (0,25:1) as follow; the weight of the powder samples (250 grams each) was extracted through maceration using a 0,25:1 ratio sample mixture, i.e., 250 grams of the sample was dissolved in 1 L of n-hexane for 48 hours with shaking and continuous stirring for proper extraction. The macerated bi-herbal sample was then sieved with a shaker and concentrated using a rotary evaporator.…”

Phytochemical Analysis from Three Different Methanolic Extracts of Red Ginger (Zingiber officinale var. Rubrum) Against LC50 Treatment of Zebra Fish as Model Fish

“…The macerated herbal samples which obtained and then concentrated using a rotary evaporator with a pressure of 1 atm, a temperature of 50°C, and a rotary rotation of 75 rpm. The extract that has thickened then can be cooled to a temperature conducive to further use (Benjamin et al, 2020). Extracts can also be stored in the refrigerator.…”