1985
DOI: 10.1016/0378-4347(85)80058-x
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Reproducible measurement of amphotericin B in serum by high-performance liquid chromatography in alkaline buffer

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Cited by 15 publications
(8 citation statements)
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“…Although it is generally accepted 43 that DMF is an excellent solvent for amphotericin B, many workers have suggested DMSO 20,21,23 or DMSOsMeOH 19,24 for preparing the standard solutions. Our experiments on the stability with time of amphotericin B dissolved in selected solvents showed ( Table 3) that the antibiotic is much more stable in DMF than in DMSO.…”
Section: Stability Of Amphotericin B In Selected Solventssmentioning
confidence: 99%
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“…Although it is generally accepted 43 that DMF is an excellent solvent for amphotericin B, many workers have suggested DMSO 20,21,23 or DMSOsMeOH 19,24 for preparing the standard solutions. Our experiments on the stability with time of amphotericin B dissolved in selected solvents showed ( Table 3) that the antibiotic is much more stable in DMF than in DMSO.…”
Section: Stability Of Amphotericin B In Selected Solventssmentioning
confidence: 99%
“…15,16 A direct spectrophotometric method for measuring amphotericin B in whole blood and plasma has been also developed, 17 but it seriously suffers from high background absorbance mainly caused by hemoglobin. Several alternative, more specific, high-performance liquid chromatographic methods are also available, [18][19][20][21][22] but their applicability is often limited by interfering endogenous compounds, 22 low extraction efficiency, 18,19 and inadequate reproducibility necessitating use of internal standards. [18][19][20] To improve the performance characteristics, a few solid-phase extraction techniques 23,24 have been introduced recently for removing interference from serum and/or urine extracts prior to liquid chromatography.…”
mentioning
confidence: 99%
“…For determination of free Amphotericin B, this has to be achieved without disturbing or breaking the liposomes present in human plasma. High-pressure liquid chromatography (HPLC) methods were reported for the quantification of Amphotericin B in plasma or serum [6][7][8][9][10][11][12][13][14][15][16][17][18][19][20]. These methods quantified the total amount of Amphotericin B in circulation.…”
Section: Introductionmentioning
confidence: 99%
“…Although several other HPLC methods for AMB have been described elsewhere (1,3,4,6,7,9,12), most have required many steps in sample preparation (1) or have a very large coefficient of variation (7). The HPLC method of Granich et al (4) for AMB determination required a solid-phase extraction procedure for elimination of potential bilirubin interference.…”
mentioning
confidence: 99%