Repression of the Virulence of
            <i>Yersinia pestis</i>
            by an F' Plasmid

Zsigray, Robert M.; Lawton, William D.; Surgalla, Michael J.

doi:10.1128/iai.39.2.974-976.1983

Cited by 5 publications

(6 citation statements)

References 20 publications

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“…Transconjugants of Y. pestis were isolated and purified by several passages on complete indicator medium (21). The clones were initially unstable, and lac segregants appeared at a high frequency; however, the stability of each clone was increased with each successive passage.…”

Section: Resultsmentioning

confidence: 99%

“…Y. pestis EV76 and EV76 51F were employed as recipients of an F-lac plasmid from either E. coli 23.10S or E. coli CSH 23. The mating conditions and the medium used for the olation of lactose-fermenting transconjugants of EV76 51 were as described previously (21). The medium used for the selection of F-lac-containing strains of EV76 was essentially MASGF medium (6), consisting of the following ingredients (per liter): K2HPO4, 10.5 g; KH2PO4, 4.5 g; (NH4)2SO4, 1.0 g; sodium citrate, 0.5 g; MgSO4 * 7H20, 0.1 g; Na2S205, 0.1 g; glycine, 1.0 mM, DL-isoleucine, 0.5 mM; DL-valine, 0.2 mM; L-Cysteine, 0.2 mM; DL-methionine, 0.2 mM; DL-phenylalanine, 0.2 mM; lactose, 2.0 g; agar, 15.0 g (pH 7.2).…”

Section: Methodsmentioning

confidence: 99%

“…Specific alterations in the Vwa plasmid are known to affect the calcium requirement of Y. pestis, but such alterations do not necessarily prevent the expression of the VW antigens (18). Thus, calcium dependence and VW antigen production are separable traits (5,17,18,21).…”

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confidence: 99%

“…The virulence of Y. pestis for mice is obliterated in strains of Y. pestis that harbor an F'lac from Escherichia coli 23.10S (21). Although the lactose-fermenting strains of Y. pestis were shown to retain all of their virulence traits, avirulence was attributed to calcium independence.…”

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confidence: 99%

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Integration of the Vwa plasmid into the chromosome of Yersinia pestis strains harboring F' plasmids of Escherichia coli

Zsigray¹,

Hopper²,

Żukowski³

et al. 1985

Infect Immun

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Conditional virulent strains of Yersinia pestis were used as recipients of an F-lac plasmid from either Escherichia coli 23.10S or E. coli CSH23. The transconjugants of Y. pestis were calcium independent; however, calcium dependence was restored after the loss of the E. coli plasmid. The plasmid contents of several Y. pestis F-lac clones were compared with those of the parent strains and several lac segregants. Although the 6and 61-megadalton plasmids were detected in all strains tested, the Vwa plasmid was absent in the F-containing strains. The plasmid was evident, however, in the lac segregants derived from the F-lac clones. Results of Southern transfers indicated that the Vwa plasmid had integrated into the chromosome of Y. pestis strains harboring F-lac.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Integration of the Vwa plasmid into the chromosome of Yersinia pestis strains harboring F' plasmids of Escherichia coli

Zsigray¹,

Hopper²,

Żukowski³

et al. 1985

Infect Immun

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“…The virulence of the Pgm + conjugant was significantly low compared with that of virulent Yreka cells for which the LD50 was usually less than 10 organisms. Since the virulence of some Y. pestis strains has been shown to be reduced or lost by conversion to streptomycin resistance (7) or acquisition of a foreign plasmid (17), the low virulence of the Pgm+ conjugant might be due to the property of streptomycin resistance or the presence of RP4 plasmid. It is also possible that a certain gene(s) necessary for the full expression of virulence had been deleted in the transmitted Pgm factor or the recipient cells used.…”

Section: Discussionmentioning

confidence: 99%

Plasmid‐Like Properties of the Four Virulence‐Associated Factors of Yersinia pestis

Tsukano¹,

Wake²,

Sakakibara

1986

Microbiology and Immunology

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The virulence-associated factors of Yersinia pestis, which determine the abilities to produce pesticin I (Pst+), capsular fraction I antigen (Fra+), V and W antigen complex (Vwa+) and a cell-surface component for adsorption of exogenous pigments (Pgm+), were independently eliminated by cultivation of the cells in the presence of acridine orange, ethidium bromide or sodium dodecyl sulfate at a subinhibitory concentration. A virulent Y. pestis strain, Yreka, harbored at least five extrachromosomal DNA molecules of different sizes. In these molecules, a novel 1 3-megadalton DNA which was cured concomitantly with the elimination of the Fra factor was found, in addition to the known species of 7 and 44 megadaltons which were lost with the conversions to Pst-and Vwa-, respectively. Although the conversion to Pgm-could not be correlated with the lack of any proper extrachromosomal DNA, the factor was transmitted to Pgm-cells with the aid of selfconjugative RP4 plasmid. The cells acquiring the Pgm factor regained virulence for mice.Virulence of Yersinia pestis is spontaneously attenuated at an unusually high frequency (5) . The conversion to avirulence is accompanied by loss of the ability to produce bacteriocinogenic pesticin I (Pst+), capsular fraction I antigen (Fra+), V and W antigen complex (Vwa+) or a cell-surface component permitting adsorption of certain exogenous pigments (Pgm+) (5, 6). It has been reported that the property of Pst+ was transmitted to nonpesticinogenic cells during conjugation directed by an E. coli R plasmid (12). Recently, it has been shown that Y. pestis cells harbor extrachromosomal DNA molecules whose existence is associated with Pst+ or Vwa+ (2, 9, 14). Portnoy et al (13) have identified a 47-megadalton plasmid participating in Vwa+. No plasmids responsible for Fra+ and Pgm+ have been found.The present study was undertaken to clarify plasmid-like properties of the virulence-associated factors of Fra, Pgm, Pst, and Vwa. We first investigated whether these factors could be eliminated from the virulent Yreka strain by culturing the cells in the presence of acridine orange, ethidium bromide or sodium dodecyl sulfate. These chemical agents are known to inhibit multiplication of certain plasmids preferentially to host cellular growth, resulting in curing of the plasmids from the cells 837

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Study of the incompatibility and replication of the 70-kb virulence plasmid of Yersinia

Bakour

Laroche

Cornelis

1983

Plasmid

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Repression of the Virulence of Yersinia pestis by an F' Plasmid

Cited by 5 publications

References 20 publications

Integration of the Vwa plasmid into the chromosome of Yersinia pestis strains harboring F' plasmids of Escherichia coli

Integration of the Vwa plasmid into the chromosome of Yersinia pestis strains harboring F' plasmids of Escherichia coli

Plasmid‐Like Properties of the Four Virulence‐Associated Factors of Yersinia pestis

Study of the incompatibility and replication of the 70-kb virulence plasmid of Yersinia

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