“…Based on our findings in the 30-cultivars study, we suggested that the relatively lower yields of 4 some of the cultivars studied in Saskatoon could be due to either herbicide damage (herbicide 5 used for weed control), poor willow root extension due to heavy clay soils, a notable winter 6 dieback of some cultivars, moisture regime limitations during the first rotation (Hangs et al, 7 2012b), and low plant-available soil N levels . Our observations from 8 the 30-cultivars study agreed with previous reports that growing degree days (GDD, base 5°C) 9 (Kopp et al, 2001; Moukoumi et al, 2012) was a very important growth limiting factor for 10 willow SRC systems, and the much lower yields observed in the 30-cultivar study adequately 11 corresponded to the lower GDD count at these northern latitudes under Canadian climatic 12 conditions. A similar decrease in willow SRC biomass yield resulting from GDD decrease was 13 previously reported by Kopp et al (2001) In sum, the total amount of C removed from the atmosphere in two full cycles of seven 3-yr 3 willow SRC rotations in the form of ecosystem C and C in harvested biomass averaged 292 and 4 215 Mg C ha -1 (= 6.6 and 4.9 Mg C ha -1 yr -1 ) in the Prairie and the Boreal Plain ecozones of 5 Saskatchewan, respectively (Amichev et al, 2012).…”