Reply from authors: RE: Optimized platelet‐rich fibrin with the low‐speed concept: Growth factor release, biocompatibility, and cellular response

Miron, Richard J.; Choukroun, Joseph; Ghanaati, Shahram

doi:10.1002/jper.18-0329

Cited by 18 publications

(9 citation statements)

References 10 publications

(14 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…PRF was thereafter developed as a second-generation platelet concentrate with the aim of anticoagulant removal [11]. This concentrate has frequently been termed leukocyte and platelet-rich fibrin (L-PRF) owing to the desired incorporation of leukocytes most commonly using a ~700 g for 12minute protocol [12]. Since anticoagulants are removed, blood is subject to clotting over time within the blood collection tube, and it therefore becomes critical that the treating clinician begin centrifugation shortly following blood collection to separate blood layers [13].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of 24 protocols for the production of platelet-rich fibrin

Miron

Chai

Fujioka‐Kobayashi

et al. 2020

Preprint

View full text Add to dashboard Cite

Background The aim of this study was to evaluate 24 protocols for the production of platelet rich fibrin (PRF) produced via horizontal centrifugation to better understand cell separation following protocols at various times and speeds. Methods All protocols were compared utilizing a recent method to quantify cells in PRF in 1 mL sequential layers pipetted from the upper layer downwards until all 10 mL were harvested. In total, 960 complete blood counts (CBCs) were investigated. Both solid and liquid-based PRF protocols were investigated following 24 protocols involving 6 relative centrifugal force (RCF) values (100,200,400,700,1000 and 1200g) at 4 centrifugation times (3,5,8 and 12 minutes). Results In general, platelets could more easily accumulate in the upper 4 layers when compared to leukocytes owing to their lower cellular density. Protocol time seemed to have a greater impact on the final cell layer separation when compared to the effect of speed. Protocols of greater than 8 minutes at 400 g led to no leukocyte accumulation in the upper PRF layers (found specifically within the buffy coat). Protocols at or below 200 g were unable to effectively accumulate platelets/leukocytes. The optimal centrifugation speed and time for solid-PRF ranged between 400 -700 g for 8 minutes. It was noted that variability in patient baseline platelet/leukocyte/erythrocyte counts (hematocrit) significantly affected cell layer separation. This finding was more pronounced at lower centrifugation speeds. Conclusions Within the investigated ranges, a protocol of 700 g for 8 minutes presented the highest yield of platelets/leukocytes evenly distributed throughout the upper PRF layers.

show abstract

Section: Introductionmentioning

confidence: 99%

Evaluation of 24 protocols for the production of platelet-rich fibrin

Miron

Chai

Fujioka‐Kobayashi

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Moreover, size of the rotor (radius at the clot and end of the tube); rotor angulation for the tube holder; revolutions per minute and time; structure and size of tubes used to produce PRF; and centrifugation model utilized must also be considered. This standardization was recently proposed and is a way to reduce bias in studies with PRF [32,33].…”

Section: Recommendations For Further Researchmentioning

confidence: 99%

Does Platelet-Rich Fibrin Decrease Dimensional Changes and Improve Postoperative Comfort in Post-Extraction Sockets? An Overview of Systematic Reviews

et al. 2020

View full text Add to dashboard Cite

This overview aimed to evaluate the methods, quality, and outcomes of systematic reviews (SRs) conducted to investigate the effects of platelet-rich fibrin (PRF) in dental sockets on promoting bone regeneration and soft tissue healing and diminishing the incidence of pain, swelling, trismus, and alveolar osteitis after tooth extraction. An electronic search without date or language restriction was done in PubMed/MEDLINE, Cochrane, and Web of Science until March 2020. Eligibility criteria included SRs that assessed the effect of PRF for human alveolar socket preservation. The quality assessment of the included studies was performed using AMSTAR 2 guidelines. The protocol of this overview was recorded in PROSPERO under the number CRD42018089617. The search and selection process yielded 13 studies published between 2011 and 2018. The analysis of the studies showed inconclusive data for the effect of the PRF and the dimensional changes. There is no definitive evidence for the impact of using PRF alone on bone regeneration in post-extraction sockets. The use of PRF improves soft tissue healing and reduces pain, bleeding, and osteitis in post-extraction sockets.

show abstract

“…PRF was thereafter developed as a second-generation platelet concentrate with the aim of anticoagulant removal [ 11 ]. This concentrate has frequently been termed leukocyte and platelet-rich fibrin (L-PRF) owing to the desired incorporation of leukocytes most commonly using a ~ 700 g for 12-min protocol [ 12 ]. Since anticoagulants are removed, blood is subject to clotting over time within the blood collection tube, and it therefore becomes critical that the treating clinician begin centrifugation shortly following blood collection to separate blood layers [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of 24 protocols for the production of platelet-rich fibrin

et al. 2020

Self Cite

View full text Add to dashboard Cite

Background The aim of this study was to evaluate 24 protocols for the production of platelet rich fibrin (PRF) produced via horizontal centrifugation to better understand cell separation following protocols at various times and speeds. Methods All protocols were compared utilizing a recent method to quantify cells in PRF in 1 mL sequential layers pipetted from the upper layer downwards until all 10 mL were harvested. In total, 960 complete blood counts (CBCs) were investigated. Both solid and liquid-based PRF protocols were investigated following 24 protocols involving 6 relative centrifugal force (RCF) values (100, 200, 400, 700, 1000 and 1200g) at 4 centrifugation times (3, 5, 8 and 12 min). Results In general, platelets could more easily accumulate in the upper 4 layers when compared to leukocytes owing to their lower cellular density. Protocol time seemed to have a greater impact on the final cell layer separation when compared to the effect of speed. Protocols of greater than 8 min at 400g led to no leukocyte accumulation in the upper PRF layers (found specifically within the buffy coat). Protocols at or below 200g were unable to effectively accumulate platelets/leukocytes. The optimal centrifugation speed and time for solid-PRF ranged between 400 and 700g for 8 min. It was noted that variability in patient baseline platelet/leukocyte/erythrocyte counts (hematocrit) significantly affected cell layer separation. This finding was more pronounced at lower centrifugation speeds. Conclusions Within the investigated ranges, a protocol of 700g for 8 min presented the highest yield of platelets/leukocytes evenly distributed throughout the upper PRF layers.

show abstract

Reply from authors: RE: Optimized platelet‐rich fibrin with the low‐speed concept: Growth factor release, biocompatibility, and cellular response

Cited by 18 publications

References 10 publications

Evaluation of 24 protocols for the production of platelet-rich fibrin

Evaluation of 24 protocols for the production of platelet-rich fibrin

Does Platelet-Rich Fibrin Decrease Dimensional Changes and Improve Postoperative Comfort in Post-Extraction Sockets? An Overview of Systematic Reviews

Evaluation of 24 protocols for the production of platelet-rich fibrin

Contact Info

Product

Resources

About