Replicon Typing of Plasmids Carrying CTX-M or CMY β-Lactamases Circulating among
 Salmonella
 and
 Escherichia coli
 Isolates

Hopkins, Katie L.; Liébana, Ernesto; Villa, Laura; Batchelor, Miranda; Threlfall, E. John; Carattoli, Alessandra

doi:10.1128/aac.00149-06

Cited by 166 publications

(147 citation statements)

References 15 publications

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“…Association of the bla CTX-M-15 gene with IncFII replicons has been described in studies conducted in Canada, France, Spain, and the United Kingdom (5,7,8,17,19). Although we observed intercontinental dissemination of 3 major IncFII plasmid scaffolds (A, B, and C) carrying bla CTX-M-15 , similarity >70% among all variants studied and presence of genes also found in pC15-1a, a CTX-M-15 plasmid (GenBank accession no.…”

Section: Discussionmentioning

confidence: 65%

Dissemination of Clonally RelatedEscherichia coliStrains Expressing Extended-Spectrum β-Lactamase CTX-M-15

Coque

Novais

Carattoli

et al. 2008

Emerg. Infect. Dis.

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663

469

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Section: Discussionmentioning

confidence: 65%

Dissemination of Clonally RelatedEscherichia coliStrains Expressing Extended-Spectrum β-Lactamase CTX-M-15

Coque

Novais

Carattoli

et al. 2008

Emerg. Infect. Dis.

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663

469

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“…CTX-M-15 is a class A ESBL encoded by the bla CTX-M-15 gene, which has been identified in plasmids of varying size (85-200 kb) and structure, and often belonging to the FII incompatibility (Inc) group [4]. OXA-48 is an Ambler class D carbapenemase that confers resistance to penicillins and reduced susceptibility to carbapenems but does not significantly hydrolyse broad-spectrum cephalosporins [3].…”

Section: Introductionmentioning

confidence: 99%

Dissemination of multiresistant Enterobacter cloacae isolates producing OXA-48 and CTX-M-15 in a Spanish hospital

Fernández

Montero

Martinez

et al. 2015

International Journal of Antimicrobial Agents

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Twenty-one multiresistant Enterobacter cloacae isolates producing OXA-48 (n = 10), CTX-M-15 (n = 7) or both (n = 4) ␤-lactamases were detected in a Spanish hospital during a 1-year period (June 2013 to June 2014). The isolates were also resistant to non-␤-lactam antimicrobials, further complicating the therapeutic options. Genotyping of the isolates identified two major clones (ST74 and ST66) that caused prolonged outbreaks in different buildings of the hospital as well as some sporadic isolates (ST78, ST45 and ST295). Isolates belonging to clone 1 (n = 7) were carbapenem-resistant and carried the bla OXA-48 gene on a conjugative IncL/M plasmid of ca. 65 kb. Clone 2 isolates (n = 11) were resistant to cefepime and harboured the bla CTX-M-15 gene on an ca. 150-kb, non-conjugative plasmid of the IncF group, co-harbouring the qnrB and aac(6 )-Ib-cr genes encoding quinolone resistance. Four clone 2 isolates were also resistant to carbapenems owing to the co-production of OXA-48. Most of the isolates were recovered from critically ill patients and were admitted to intensive care units; a single patient was transferred from another Spanish hospital. Intrahospital and interhospital dissemination of multiresistant E. cloacae isolates is of major clinical concern as it could lead to endemic nosocomial situations.

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“…On the other hand, plasmids harboring aphA1, aadA1, tetA, and sul1 were IncP type. Previous reports indicated that IncI1 plasmids harboring bla CTX-M-14 were detected in Salmonella enterica serovar Enteritidis (S. Enteritidis) [7] and E. coli bacteria [15]. It's worth noting that several studies describing the occurrence of CTX-M-14 ESBL in S. Enteritidis isolated from human patients in Spain [17], Hong Kong [10] and Japan [9] have been reported since 2003.…”

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confidence: 99%

Emergence of Salmonella enterica Serovar Infantis Harboring IncI1 Plasmid with blaCTX-M-14 in a Broiler Farm in Japan

Kameyama¹,

Chuma

Yokoi

et al. 2012

J. Vet. Med. Sci.

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ABSTRACT. Cefotaxime (CTX)-resistant and -susceptible Salmonella enterica serovar Infantis isolates obtained from broilers raised on a farm in January 2010 in Japan were characterized to establish their resistance determinants. The CTX-resistant isolates produced CTX-M-14 extended-spectrum β-lactamase and harbored 2 distinct plasmid of approximately 140-and 95-kb, whereas the CTX-susceptible isolates harbored one 140-kb plasmid. The 95-kb plasmids were replicon typed as IncI1 carrying the bla CTX-M-14 gene, while the 140-kb plasmids were IncP and harbored the aphA1, aadA1, tetA, and sul1 genes. Genetic fingerprinting by pulsed-field gel electrophoresis revealed similar macrorestriction profiles amongst CTX-resistant and susceptible isolates, suggesting a clonal relationship. The presence of CTX-resistant S. Infantis on a broiler farm has occurred through the acquisition of IncI1 resistance plasmid. KEY WORDS: β-lactamase, broiler, CTX-M-14, extended-spectrum plasmid replicon typing, Salmonella Infantis.doi: 10.1292/jvms.11-0488; J. Vet. Med. Sci. 74(9): 1213-1216, 2012 Amongst members of the Enterobacteriaceae family, Escherichia coli (E. coli) and Klebsiella pneumoniae have been implicated as potential producers of extendedspectrum β-lactamases (ESBLs) which possess hydrolyzing activity against third-generation cephalosporins. In recent years, several reports have described other bacterial species within the Enterobacteriaceae family capable of producing ESBLs. Of these, Salmonella spp. producing ESBLs have been detected in salmonellosis cases both in humans [9,10,17] and domestic animals [18].Salmonella enterica serovar Infantis (S. Infantis) is one of the predominant serotypes isolated from broilers in Japan [19]. In the late 1990s, S. Infantis isolates harboring ESBLs were recovered from hospitalized patients in South America [14]. More recently, S. Infantis producing ESBLs were found not only in human patients but also in domestic animals and commercial meats [3,5]. Most of these produced the CTX-M-type ESBLs, indicating the probability that dissemination of 3rd-generation cephalosporin-resistant S. Infantis associated with various types of β-lactamases is gradually expanding world-wide. In this article, we characterized S. Infantis isolates carrying CTX-M-14 ESBL derived from broiler chickens.Ten S. Infantis isolates obtained from 30 broiler cecal samples collected at a poultry processing plant in western Japan in January 2010 were used in this study. The broiler chickens involved in this survey were sampled from 3 different flocks raised on the same commercial farm. The isolation of the bacteria was done as follows; approximately 1 g of cecal contents was aseptically mixed with 5 ml of sterilized distilled water. Then, 1 ml of suspension was enriched in 10 ml of tetrathionate broth (Merck KGaA, Darmstadt, Germany) and incubated at 42°C. After 24 hr of incubation, a loopful from each of enriched broth was streaked onto plates of selective deoxycholate hydrogen sulfide lactose (DHL) agar (Oxoid Ltd., Basing...

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Replicon Typing of Plasmids Carrying CTX-M or CMY β-Lactamases Circulating among Salmonella and Escherichia coli Isolates

Cited by 166 publications

References 15 publications

Dissemination of Clonally RelatedEscherichia coliStrains Expressing Extended-Spectrum β-Lactamase CTX-M-15

Dissemination of Clonally RelatedEscherichia coliStrains Expressing Extended-Spectrum β-Lactamase CTX-M-15

Dissemination of multiresistant Enterobacter cloacae isolates producing OXA-48 and CTX-M-15 in a Spanish hospital

Emergence of <i>Salmonella enterica</i> Serovar Infantis Harboring IncI1 Plasmid with <i>bla</i><sub>CTX-M-14</sub> in a Broiler Farm in Japan

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