Dissemination of Clonally Related<i>Escherichia coli</i>Strains Expressing Extended-Spectrum β-Lactamase CTX-M-15

Coque, Teresa M.; Novais, Ângela; Carattoli, Alessandra; Poirel, Laurent; Pitout, Johann; Peixe, Luı́sa; Baquero, Fernando; Cantón, Rafael; Nordmann, Patrice

doi:10.3201/eid1402.070350

Cited by 663 publications

(543 citation statements)

References 38 publications

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“…ST131 is prevalent in most parts of the world, and is often associated with fluoroquinolone resistance and the production of CTX-M-15. ST405 with various types of CTX-M b-lactamases also has a worldwide distribution but is not as prevalent as ST131 (Coque et al, 2008;Jones et al, 2008;Mihaila et al, 2010;Smet et al, 2010). These reports suggest that the intercontinental dissemination of ST131, and to a lesser extent ST405, has in part contributed to the worldwide emergence of CTX-M-producing E. coli.…”

Section: Introductionmentioning

confidence: 77%

Virulence potential and adherence properties of Escherichia coli that produce CTX-M and NDM β-lactamases

Peirano

Mulvey

Armstrong

et al. 2013

Journal of Medical Microbiology

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The success of certain sequence types such as ST131 that produce CTX-M or NDM blactamases, and ST405 that produce CTX-M b-lactamases, among extraintestinal Escherichia coli (ExPEC) had previously been linked to a combination of antimicrobial resistance and certain virulence factors. The adherence properties of these sequence types to gastro-intestinal epithelial cells had not been investigated. A study was therefore designed to investigate the phylogenetic groups, virulence factors and adherence properties of E. coli sequence types ST101, ST131 and ST405 that produce CTX-M-15 and NDM-1. Our results show that ST131 was positive for phylogenetic group B2, ST101 for B1 and ST404 for D. ST131 had more virulence factors than ST101 or ST405. Interestingly, ST101 adhered more avidly to HEp-2 and Caco-2 cells than did ST131 and ST405. Our study showed that adherence to gastro-intestinal cells did not seem to play an important role in the worldwide epidemiological success of ST131 and ST405. The exact role of ExPEC-associated virulence genes is unknown and it is unlikely that one set of factors determines the virulence properties and epidemiological success of certain sequence types. Future investigations should be undertaken to study the microbiological and ecological factors that make certain sequence types among ExPEC such successful pathogens. INTRODUCTIONEscherichia coli is an important cause of community and hospital-associated human infections and is responsible for considerable morbidity, mortality and increased health costs (Russo & Johnson, 2003). The management of infections due to extraintestinal E. coli (ExPEC) has been complicated by the emergence of antimicrobial resistance, especially since the late 1990s .b-Lactamases are bacterial enzymes that inactivate blactam antibiotics by hydrolysis, which results in ineffective compounds. Most important within ExPEC is the increasing recognition of isolates producing 'newer' b-lactamases that consist of extended-spectrum b-lactamases (ESBLs) (e.g. CTX-M types) and carbapenemases [e.g. metallo-blactamases (MBLs) such as the NDMs, serine b-lactamases such as KPCs and OXA-48-like enzymes] (Pitout, 2012). Since the late 1990s, CTX-M types of ESBL enzymes have emerged worldwide among E. coli, and have become the most widespread type of ESBL in the world . A new type of MBL, named NDM, has been described in Klebsiella pneumoniae and E. coli recovered from a Swedish patient who was previously hospitalized in New Delhi, India (Yong et al., 2009). Recent reports from the Indian subcontinent (including India, Pakistan and Bangladesh) show that the distribution of NDM b-lactamases among Enterobacteriaceae is widespread in these countries, while sporadic cases of infections due to bacteria with these enzymes have been reported from different parts of the world, including several countries in Europe, North America, the Middle East, Asia, Africa and Australia (Nordmann et al., 2011).Multilocus sequence typing (MLST), which uses sequence variation in a number of housekeeping gen...

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Section: Introductionmentioning

confidence: 77%

Virulence potential and adherence properties of Escherichia coli that produce CTX-M and NDM β-lactamases

Peirano

Mulvey

Armstrong

et al. 2013

Journal of Medical Microbiology

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“…b-Lactams (mainly extended-spectrum cephalosporins and carbapenems) and fluoroquinolones constitute the main therapeutic choices to treat infections caused by these microorganisms. However, resistance to these compounds has been reported increasingly frequently from different parts of the world in recent years (Cantó n et al, 2008;Reinert et al, 2007). The last European Antibiotic Resistance Surveillance System (http://www.ecdc.europa.eu/en/ activities/surveillance/EARS-Net) report from 2006, covering over 800 laboratories from 31 countries, showed a continuous increase since 2000 in invasive E. coli and K. pneumoniae isolates resistant to third-generation cephalosporins, with a prevalence of .10% for half of the enrolled countries.…”

Section: Discussionmentioning

confidence: 99%

“…the ESBL producers were resistant to different antibiotic families including -besides b-lactamsfluoroquinolones, aminoglycosides and trimethoprim/sulfamethoxazole (Lahlou Amine et al, 2009;Minarini et al, 2007b;Mshana et al, 2009;Pitout et al, 2004;Sekhsokh et al, 2008;Shi et al, 2009), which contribute to the selection and persistence of multidrug-resistant ESBL strains and plasmids in both clinical and community settings (Cantó n et al, 2008). Nevertheless, all CTX-Mproducing strains were susceptible to imipenem.…”

Section: A Barguigua and Others 1348mentioning

confidence: 99%

Characterization of extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae isolates from the community in Morocco

Barguigua

Otmani

Talmi

et al. 2011

Journal of Medical Microbiology

104

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Of 803 community Escherichia coli (n5767) and Klebsiella pneumoniae (n536) isolates collected from patients with urinary tract infections in three Moroccan cities, 10 E. coli (1.3%) and 2 K. pneumoniae (5.6 %) isolates were shown to produce extended-spectrum b-lactamases (ESBLs). PFGE revealed that the E. coli isolates comprised seven distinct genotypes. The presence of plasmids in the 12 isolates was revealed by conjugation experiments of plasmids from these Enterobacteriaceae strains with E. coli K 12 J 5 , with further isolation of the plasmids in the transconjugants. Subsequent nucleotide sequencing indicated that the plasmids encoded the bla CTX-M , bla OXA , bla TEM and bla SHV genes, including genes for CTX-M-15 (n511), OXA-1 (n511), TEM-1b (n54), SHV-5 (n51) and SHV-1 (n52). Identification of plasmid-mediated quinolone-resistance genes was performed by PCR. The aac(69)Ib-cr variant was detected in all strains, and two strains co-expressed qnrS1, bla CTX-M-15 and bla OXA-1 genes. The presence of ESBLs in the Enterobacteriaceae strains studied was probably due to the dissemination of resistance plasmids with the predominant genotype of bla CTX-M-15.

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“…Moreover, other observations indicate that our past experiments may have been inappropriate to conclude that Kid does not kill Escherichia coli cells (30,31). Importantly, Kid, Kis, and other elements that we found essential for R1 rescue are conserved in plasmids conferring resistance to extended-spectrum β-lactamases, a worrying threat to human health (1,(6)(7)(8)(9)(10)32). Therapeutic options to fight pathogens carrying these plasmids are limited, and activation of Kid may be perceived as a good antibiotic alternative.…”

Section: Significancementioning

confidence: 88%

“…Kis-kid and hok-sok function sequentially and coordinately to facilitate survival of plasmid R1. When R1 CNs ensure plasmid transmission to both descendant cells, Kid remains neutralized and parental cells proliferate normally (1). However, Kid becomes active when R1 copy numbers are insufficient to warrant plasmid transmission to both daughter cells.…”

Section: Kid Inhibits Cell Division In E Coli and Does Not Halt Protmentioning

confidence: 99%

Toxin Kid uncouples DNA replication and cell division to enforce retention of plasmid R1 in Escherichia coli cells

Pimentel

Nair

Bermejo-Rodríguez

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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Worldwide dissemination of antibiotic resistance in bacteria is facilitated by plasmids that encode postsegregational killing (PSK) systems. These produce a stable toxin (T) and a labile antitoxin (A) conditioning cell survival to plasmid maintenance, because only this ensures neutralization of toxicity. Shortage of antibiotic alternatives and the link of TA pairs to PSK have stimulated the opinion that premature toxin activation could be used to kill these recalcitrant organisms in the clinic. However, validation of TA pairs as therapeutic targets requires unambiguous understanding of their mode of action, consequences for cell viability, and function in plasmids. Conflicting with widespread notions concerning these issues, we had proposed that the TA pair kis-kid (killing suppressor-killing determinant) might function as a plasmid rescue system and not as a PSK system, but this remained to be validated. Here, we aimed to clarify unsettled mechanistic aspects of Kid activation, and of the effects of this for kis-kid-bearing plasmids and their host cells. We confirm that activation of Kid occurs in cells that are about to lose the toxin-encoding plasmid, and we show that this provokes highly selective restriction of protein outputs that inhibits cell division temporarily, avoiding plasmid loss, and stimulates DNA replication, promoting plasmid rescue. Kis and Kid are conserved in plasmids encoding multiple antibiotic resistance genes, including extended spectrum β-lactamases, for which therapeutic options are scarce, and our findings advise against the activation of this TA pair to fight pathogens carrying these extrachromosomal DNAs.PemK | mRNA interferase | parD | plasmid stability | RNase

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Dissemination of Clonally RelatedEscherichia coliStrains Expressing Extended-Spectrum β-Lactamase CTX-M-15

Abstract: E . coli ST131 and ST405 and multidrug-resistant IncFII plasmids may determine spread of this lactamase.

Cited by 663 publications

References 38 publications

Virulence potential and adherence properties of Escherichia coli that produce CTX-M and NDM β-lactamases

Virulence potential and adherence properties of Escherichia coli that produce CTX-M and NDM β-lactamases

Characterization of extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae isolates from the community in Morocco

Toxin Kid uncouples DNA replication and cell division to enforce retention of plasmid R1 in Escherichia coli cells

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