Replication pattern of psittacosis virus in a tissue culture system

Tanami, Yoh; Pollard, Morris; Starr, Theodore J.

doi:10.1016/0042-6822(61)90072-1

Cited by 23 publications

(17 citation statements)

References 8 publications

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“…Spheroplasts induced by treatment of Gram-negative bacteria with penicillin are swollen and vacuolated structures, osmotically labile and dependent on a medium of high tonicity for stabilization (Lederberg & St Clair, 1958;McQuillen, 1960;Martin, 1963). At the fine structural level (Thorsson & Weibull, 1958;Hines, Freeman & Pearson, 1964;Weibull & Mohri, 1965) spheroplasts are markedly pleomorphic, but often bear a close resemblance to the penicillin forms seen in the present study.…”

Section: Discussionsupporting

confidence: 67%

“…The compound evidently interferes also with synthesis of bacterial cell walls (Tomasz & Borek, 1960;Rogers & Perkins, 1960). In the present study the effect of 5-FU is seen mainly in the later stages of the LGV growth cycle.…”

Section: Discussionmentioning

confidence: 99%

“…In cytochemical studies using the acridine orange fluorescence technique (Pollard, Starr, Tanami & Elliott, 1960;Tanami, Pollard & Starr, 1961 ;Pollard & Starr, 1962) it was observed that treatment of cell cultures with 5-FU 14 hr after inoculation with PLT agents resulted in highly abnormal patterns of diffuse RNA-containing material in the region of the cytoplasmic inclusions. This was interpreted as host-cell-contributed material.…”

Section: Discussionmentioning

confidence: 99%

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Fine Structure of Lymphogranuloma Venereum Agent and the Effects of Penicillin and 5-Fluorouracil

Armstrong

Reed

1967

Journal of General Microbiology

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Section: Discussionsupporting

confidence: 67%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Fine Structure of Lymphogranuloma Venereum Agent and the Effects of Penicillin and 5-Fluorouracil

Armstrong

Reed

1967

Journal of General Microbiology

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“…This is difficult to reconcile with a specific FUDR inhibition of thymidylate synthetase (see Rich, Saslaw & Eidinhoff, 1960). Furthermore, the conclusion of Tanami et al (1961) that psittacosis DNA synthesis occurs relatively early is at variance with the observations of Becker, Mashiah & Bernkopf (1962) that most DNA synthesis (incorporation of labelled thymidine) occurred late in the intracellular developmental cycle of the related trachoma organism.…”

Section: Discussionmentioning

confidence: 99%

Cell-wall Constituents of Rickettsiae and Psittacosis-Lymphogranuloma Organisms

Perkins¹,

Allison

1963

Journal of General Microbiology

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SUMMARYThe taxonomic position of organisms belonging to the rickettsiae and psittacosis-lymphogranuloma groups is controversial. Although, like viruses, all pathogenic forms of these organisms are obligate intracellular parasites, in other respects they resemble bacteria. Since a cell wall, deriving its rigidity from mucopeptide, which contains the amino sugar muramic acid as a key constituent, has so far only been found in bacteria and the closely related blue-green algae, the presence of muramic acid in an organism may be used as a taxonomic criterion. The mucopeptides of bacterial cell walls are also often sensitive to lysozyme, so that dissolution by this enzyme serves as an indication of the presence of mucopeptide. Organisms of the rickettsiae and psittacosis-lymphogranuloma groups have been examined for the presence of muramic acid. Critical chemical tests have shown that this substance is present in organisms of both groups. Cell walls of Rickettsia burnetii were dissolved by lysozyme. In the light of these and other results the taxonomic position of these groups of organisms is discussed.

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“…of rectifying the nutritional deficiency. Tanami, Pollard & Starr (1961) studied the effects of pyrimidine analogues and p-fluorophenylalanine on the growth curve of psittacosis virus, also in tissue cultures. It was inferred that a lag of 10 hr.…”

Section: Introductionmentioning

confidence: 99%

Structure and Replication of the Trachoma Agent in Cell Cultures, as shown by Electron Microscopy

Armstrong

Valentine

Fildes

1963

Journal of General Microbiology

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SUMMARYPropagation of the trachoma agent in human cell cultures has made possible the observation by electron microscopy of sequential stages in the replication of the micro-organism. Scattered reticular foci with incomplete limiting membranes were present in the cytoplasm of HeLa cells 12 hr. after exposure to the infectious agent in high multiplicity. After 24 hr. the foci had coalesced to give a single reticular mass. Within it there appeared large and discrete developmental forms from which, by progressive subdivision and diminution in size, !mica1 elementary bodies were formed. The elementary bodies had a prominent limiting membrane, and a dense core within which a fibrillar nucleoid could be recognized. The controversial taxonomic status of the trachoma agent is considered briefly in the light of ultrastructural evidence.

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Replication pattern of psittacosis virus in a tissue culture system

Cited by 23 publications

References 8 publications

Fine Structure of Lymphogranuloma Venereum Agent and the Effects of Penicillin and 5-Fluorouracil

Fine Structure of Lymphogranuloma Venereum Agent and the Effects of Penicillin and 5-Fluorouracil

Cell-wall Constituents of Rickettsiae and Psittacosis-Lymphogranuloma Organisms

Structure and Replication of the Trachoma Agent in Cell Cultures, as shown by Electron Microscopy

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