Replication of the bovine immunodeficiency-like virus in diploid and aneuploid cells: Permanent, latent and virus-productive infections in vitro

Bouillant, A. M. P.; Ruckerbauer, G M; Nielsen, K.

doi:10.1016/s0923-2516(89)80138-4

Cited by 15 publications

(5 citation statements)

References 73 publications

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“…The bovine lentivirus, BIV, is difficult to culture in vitro [3,4,15,28], producing minimal amounts of viral proteins. This has complicated development of assays for BIV-specific antibodies.…”

Section: Discussionmentioning

confidence: 99%

A Western blot assay for the detection of antibodies to bovine immunodeficiency-like virus in experimentally inoculated cattle, sheep, and goats

Whetstone¹,

VanDerMaaten

Miller

1991

Archives of Virology

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A cocultivation method was used to establish a cytocidal bovine immunodeficiency-like virus (BIV) infection in primary fetal bovine lung (FBL) cell cultures. Cultures were monitored for virus production using radial immunodiffusion and agar gel immunodiffusion. Pelleted virus and detergent (CHAPS)-solubilized infected cell lysates from BIV-infected cell cultures were compared as sources of antigen for Western blots. Pelleted virus preparations from FBL-BIV cell cultures produced the best antigen for Western blot. Sheep and goats were inoculated with BIV and serum antibody responses were monitored up to 1 year post inoculation (PI). Sera from experimentally infected cattle, sheep, and goats reacted in Western blot assay with BIV viral induced polypeptides gp 110, p 72, p 55, p 50, gp 42, p 38, p 26, p 24, p 18, p 15, and p 13. Antibodies to p 26 were detected as early as 2 weeks PI in cattle, sheep, and goats. Antibodies to gp 110 were detected by 4 to 6 weeks PI in cattle, and by 9 months PI in sheep and goats. Antibodies to BIV proteins were still evident in cattle sera 2 1/2 years PI, and in sheep and goat sera 1 year PI.

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Section: Discussionmentioning

confidence: 99%

A Western blot assay for the detection of antibodies to bovine immunodeficiency-like virus in experimentally inoculated cattle, sheep, and goats

Whetstone¹,

VanDerMaaten

Miller

1991

Archives of Virology

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“…In addition, established canine thymus (Cf2Th), embryonic rabbit epithelium (EREp) and various other bovine cell lines have been used for infecting the virus [36,40,42]. Only Cf2Th cell line is known to sustain a long-term productive infection [16,36,42]. In peripheral blood mononuclear cells (PBMC) collected from naturally infected animals, BIV has been shown to infect and transcribe its genome in different subsets of cells-CD3?, CD4?, CD8?…”

Section: Like Other Lentiviruses Biv Infect Cells Of the Immune Systmentioning

confidence: 99%

Bovine immunodeficiency virus: a lentiviral infection

2013

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“…In vivo, BIV DNA was detected in a large variety of bovine tissues, including brain, lungs, lymph nodes, spleen, peripheral blood mononuclear cells (PBMC) and semen of infected animals (Gonda et al, 1990;Pifat et al, 1992;Baron et al, 1995Baron et al, , 1998Nash et al, 1995a;Zhang et al, 1997b;Gradil et al, 1999). BIV replicated in vitro in a wide variety of cells, such as bovine, ovine, rabbit and canine cells (Bouillant et al, 1989;Gonda et al, 1990;Zhang et al, 1997b), but not human cells (Kashanchi et al, 1991;Whetstone et al, 1992). BIV induced a cytopathic effect characterized by the formation of syncytia in permissive cells.…”

Section: Transmission Cell Tropism and Host Range Of Bivmentioning

confidence: 99%

The molecular biology of bovine immunodeficiency virus: a comparison with other lentiviruses

St-Louis

Cojocariu

Archambault

2004

Anim. Health. Res. Rev.

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Bovine immunodeficiency virus (BIV) was first isolated in 1969 from a cow, R-29, with a wasting syndrome. The virus isolated induced the formation of syncytia in cell cultures and was structurally similar to maedi-visna virus. Twenty years later, it was demonstrated that the bovine R-29 isolate was indeed a lentivirus with striking similarity to the human immunodeficiency virus. Like other lentiviruses, BIV has a complex genomic structure characterized by the presence of several regulatory/accessory genes that encode proteins, some of which are involved in the regulation of virus gene expression. This manuscript aims to review biological and, more particularly, molecular aspects of BIV, with emphasis on regulatory/accessory viral genes/proteins, in comparison with those of other lentiviruses.

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Replication of the bovine immunodeficiency-like virus in diploid and aneuploid cells: Permanent, latent and virus-productive infections in vitro

Cited by 15 publications

References 73 publications

A Western blot assay for the detection of antibodies to bovine immunodeficiency-like virus in experimentally inoculated cattle, sheep, and goats

A Western blot assay for the detection of antibodies to bovine immunodeficiency-like virus in experimentally inoculated cattle, sheep, and goats

Bovine immunodeficiency virus: a lentiviral infection

The molecular biology of bovine immunodeficiency virus: a comparison with other lentiviruses

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