Replication of mouse hepatitis virus: negative-stranded RNA and replicative form RNA are of genome length

Lai, Michael M. C.; Patton, C. D.; Stohlman, Stephen A.

doi:10.1128/jvi.44.2.487-492.1982

Cited by 95 publications

(74 citation statements)

References 15 publications

(33 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A stretch of highly conserved nucleotides about 80 bases from the 3' end of the genome has been identified as a negative-strand initiation signal (Lai, 1990). Traditionally it was thought that new positive-sense, genomiclength RNA as well as positive-sense, subgenomic mRNAs were all transcribed off a genomic-length RI (Lai, et al, 1982). However, results from recent studies of TGEV (Sethna, et al, 1989;Sethna, et al, 1991 ), MHV (Sawicki and Sawicki, 1990), and BCV (Liu, et al, 1991) have demonstrated transcription via subgenomic RIs as well.…”

Section: Coronavirus Genetics and Replicationmentioning

confidence: 99%

A review of feline infectious peritonitis virus: molecular biology, immunopathogenesis, clinical aspects, and vaccination

Olsen

1993

Veterinary Microbiology

111

View full text Add to dashboard Cite

Feline infectious peritonitis (FIP) has been an elusive and frustrating problem for veterinary practitioners and cat breeders for many years. Over the last several years, reports have begun to elucidate aspects of the molecular biology of the causal virus (FIPV). These papers complement a rapidly growing base of knowledge concerning the molecular organization and replication of coronaviruses in general. The fascinating immunopathogenesis of FIPV infection and the virus' interaction with macrophages has also been the subject of several recent papers. It is now clear that FIPV may be of interest to scientists other than veterinary virologists since its pathogenesis may provide a useful model system for other viruses whose infectivity is enhanced in the presence of virus-specific antibody. With these advances and the recent release of the first commercially-available FIPV vaccine, it is appropriate to review what is known about the organization and replication of coronaviruses and the pathogenesis of FIPV infection.

show abstract

Section: Coronavirus Genetics and Replicationmentioning

confidence: 99%

A review of feline infectious peritonitis virus: molecular biology, immunopathogenesis, clinical aspects, and vaccination

Olsen

1993

Veterinary Microbiology

111

View full text Add to dashboard Cite

show abstract

“…6B and 6E. Since all MHV-specific mRNAs are transcribed from the same negative template of genomic RNA (Lai et a/., 1982b), the kinetics of RNA synthesis for all mRNAs vs virus dilution follows a one-hit kinetics. Similarly, the synthesis of DlssA decreased linearly following virus dilution, according to a one-hit kinetics (Figs.…”

Section: Dependence or Independence Of Helper Functions For Di-specifmentioning

confidence: 99%

“…In infected cells, the genomic RNA of MHV encodes an RNA-dependent RNA polymerase (Brayton et a/., 1982(Brayton et a/., , 1984Mahy et al, 1983) which is utilized for the synthesis of a genomic-sized negative-stranded RNA (Lai et a/., 198213). The negative-stranded RNA then serves as the template for the synthesis of six subgenomic and one genome-sized mRNA (Brayton et al, 1984;Lai et al, 1982b). These mRNAs are arranged in the form of a 3' coterminal "nested" set, i.e., the sequences of each mRNA are contained entirely within the next larger mRNA (Lai et a/., 1981;Leibowitz et a/,, 1981).…”

Section: Introductionmentioning

confidence: 99%

Defective-interfering particles of murine coronavirus: Mechanism of synthesis of defective viral RNAs

et al. 1988

View full text Add to dashboard Cite

The mechanism of synthesis of the defective viral RNAs in cells infected with defective-interfering (DI) particles of mouse hepatitis virus was studied. Two DI-specific RNA species, DIssA of genomic size and DIssE of subgenomic size, were detected in DI-infected cells. Purified DI particles, however, were found to contain predominantly DIssA and only a trace amount of DIssE RNA. Despite its negligible amount, the DIssE RNA in virions appears to serve as the template for the synthesis of DIssE RNA in infected cells. This conclusion was supported by two studies. First, the uv target size for DIssE RNA synthesis is significantly smaller than that for DIssA. Second, when purified DIssE RNA was transfected into cells which had been infected with a helper virus, DIssE RNA could replicate itself and became a predominant RNA species in the infected cells. Thus, DIssE RNA was not synthesized from the genomic RNA of DI particles. By studying the relationship between virus dilution and the amount of intracellular viral RNA synthesis, we have further shown that DIssE RNA synthesis requires a helper function, but it does not utilize the leader sequence of the helper virus. In contrast, DIssA synthesis appears to be helper-independent and can replicate itself. Thus DIssA codes for a functional RNA polymerase.

show abstract

“…3). This assumption is based on the characterization of genomic and subgenomic mRNAs of the coronavirus murine hepatitis virus (MHV) (Lai and Stohlman, 1981;Lai et al, 1982) and the related equine torovirus (van Vliet et al, 2002) belonging also to the Coronaviridae family.…”

Section: The Sars-cov Rna Cap Structure Formationmentioning

confidence: 99%

SARS-CoV ORF1b-encoded nonstructural proteins 12–16: Replicative enzymes as antiviral targets

et al. 2014

View full text Add to dashboard Cite

The SARS (severe acute respiratory syndrome) pandemic caused ten years ago by the SARS-coronavirus (SARS-CoV) has stimulated a number of studies on the molecular biology of coronaviruses. This research has provided significant new insight into many mechanisms used by the coronavirus replication-transcription complex (RTC). The RTC directs and coordinates processes in order to replicate and transcribe the coronavirus genome, a single-stranded, positive-sense RNA of outstanding length (∼27-32kilobases). Here, we review the up-to-date knowledge on SARS-CoV replicative enzymes encoded in the ORF1b, i.e., the main RNA-dependent RNA polymerase (nsp12), the helicase/triphosphatase (nsp13), two unusual ribonucleases (nsp14, nsp15) and RNA-cap methyltransferases (nsp14, nsp16). We also review how these enzymes co-operate with other viral co-factors (nsp7, nsp8, and nsp10) to regulate their activity. These last ten years of research on SARS-CoV have considerably contributed to unravel structural and functional details of one of the most fascinating replication/transcription machineries of the RNA virus world. This paper forms part of a series of invited articles in Antiviral Research on "From SARS to MERS: 10years of research on highly pathogenic human coronaviruses".

show abstract

Replication of mouse hepatitis virus: negative-stranded RNA and replicative form RNA are of genome length

Cited by 95 publications

References 15 publications

A review of feline infectious peritonitis virus: molecular biology, immunopathogenesis, clinical aspects, and vaccination

A review of feline infectious peritonitis virus: molecular biology, immunopathogenesis, clinical aspects, and vaccination

Defective-interfering particles of murine coronavirus: Mechanism of synthesis of defective viral RNAs

SARS-CoV ORF1b-encoded nonstructural proteins 12–16: Replicative enzymes as antiviral targets

Contact Info

Product

Resources

About