Replication of Borna disease virus in cell cultures

Herzog, S.; Rott, R.

doi:10.1007/bf02122849

Cited by 185 publications

(150 citation statements)

References 3 publications

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“…The Giessen strain Hc/80 of BDV was used throughout this study [6,9], Four-to-live v^-eek old female Lewis rals were obtained from the Zenlralinslitut fur Versuehstierzucht (Hannover, Germany), and were infected via intranasal route with 6x10-' IDso ofthe virus.…”

Section: Virus and Animalsmentioning

confidence: 99%

T cell memory specific for self and non-self antigens in rats persistently infected with Borna disease virus

Rott

Herzog

Cash

1993

Clinical and Experimental Immunology

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SUMMARYWe have studied CD4' Thl Tcell responses in Borna disease (BD). a virus-mediated immune disease of the central nervous system (CNS), and demonstrate the priming of virus-specific as well as autoreacti veT cells specilicf or myclin antigens in the course of viral infection. The fate of these//H'/ro generated T cells was subsequently assessed by in vitro proliferation assays with lymphocytes from different lymphoid organs of diseased animals over a long period of time. Virus-specilie T cell responses continuously decreased during the establishment of persistent infection and could no longer be detected after 5-6 months/'o.sf infectionem. when inflammatory reactions in Ihe brain had ceased. By contrast, autoantigen-specific T celts kepi their ability to mount characteristic secondary responses-although at an overall rather low level-over long periods of time: these autoreactive T cellshomed to a specihc lymphoid organ, the perithymic lymph node. Our study thus describes for the first time a complete decline of virus-specific Tcell metiiory in a persistent viral infection, and raises the question how long-lasling Tcell autoreactivity is controlled.

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Section: Virus and Animalsmentioning

confidence: 99%

T cell memory specific for self and non-self antigens in rats persistently infected with Borna disease virus

Rott

Herzog

Cash

1993

Clinical and Experimental Immunology

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“…The knowledge of BDV has improved thanks to the establishment of persistently infected cell lines [30,41]. The first reference strain (strain V) isolated in 1929, was obtained from a brain homogenate of a diseased horse, after a series of passages on the rabbit [19].…”

Section: Classification Of Bdvmentioning

confidence: 99%

“…Western blot [31], ELISA [19] and immunofluorescence assay (IFA) [30] can be used. IFA is acknowledged to be the most reliable method for the detection of BDV-specific antibodies, even though comparative interassays have shown very variable results, partly due to variable sensitivity linked to the use of different cell systems [62].…”

Section: Serological Diagnosismentioning

confidence: 99%

Borna disease: current knowledge and virus detection in France

Dauphin¹,

Legay

Pitel

et al. 2002

Vet. Res.

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-For over two centuries, Borna disease (BD) has been described as a sporadically occurring infectious meningoencephalomyelitis affecting horses and sheep in Central Europe. Over the last decade, the BD epidemiology has been discussed. Firstly, its geographical distribution seems larger than what was previously thought. Secondly, the disease can affect a large number of warm-blooded animal species, including humans. The aetiological agent is the Borna disease virus (BDV), an enveloped, nonsegmented negative-stranded RNA virus classified in the new virus family Bornaviridae (Mononegavirales order). It can induce severe clinical signs of encephalitis with striking behavioural disturbances and may cause death. BDV genome has recently been detected in France in the blood and brain of several animal species (horses, bovines, foxes). meningoencephalomyelitis / Borna disease virus / epidemiology / diagnosisRésumé -La maladie de Borna : connaissances actuelles et détection du virus en France. La maladie de Borna est connue depuis plus de deux siècles en Europe centrale comme une méningoencé-phalomyélite d'origine infectieuse affectant les chevaux et les moutons. Depuis ces dix dernières années, l'épidémiologie de la maladie a été révisée puisque la répartition géographique de la maladie de Borna s'avère plus large que rapportée jusqu'alors. En outre, elle peut affecter un grand nombre d'espèces animales à sang chaud, y compris l'homme. L'agent étiologique est le BDV (virus de la maladie de Borna), virus enveloppé, à ARN simple brin, non segmenté et de polarité négative, récem-ment classifié dans la nouvelle famille des Bornaviridae, (ordre des Mononegavirales). Le BDV peut induire des signes cliniques sévères d'encéphalite virale avec des troubles comportementaux importants et il peut entraîner la mort de l'animal. Récemment, le génome du BDV a été mis en évidence en France dans le sang et le cerveau de plusieurs espèces animales (chevaux, bovins, renards). méningoencéphalomyélite / virus de la maladie de Borna / épidémiologie / diagnostic 127

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“…Persistently BDV-infected and uninfected MDCK cells were used for indirect immunofluorescence assay as previously described (Herzog & Rott, 1980). ' Oligo ', a human oligodendrocyte cell line (Pauli & Ludwig, 1985 ; kindly provided by G. Pauli, Berlin, Germany) was infected with a retina-derived BDV strain from a naturally infected horse.…”

Section: Cegamentioning

confidence: 99%