Replication, C and Ag-NOR chromosome-banding in two anguilliform fish species

Amores, Angel; Béjar, Julia; Alvarez, M. C.

doi:10.1007/bf00349129

Cited by 12 publications

(5 citation statements)

References 22 publications

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“…Low diploid numbers (2n = 38) and a high number of meta-submetacentric chromosomes seem to be the most common condition for Ophichthidae species ( Table 1 ). Species of the families Muraenesocidae, Congridae ( Salvadori et al , 1994 ), Anguillidae ( Sola et al , 1980 ; Sola et al , 1984 ) and Echelidae ( Amores et al , 1995 ) also share a similar karyotypic pattern. Phylogenetic affinities based on the C- and G-banding patterns have also been proposed for the families Anguillidae and Congridae ( Salvadori et al , 1994 ).…”

Section: Discussionmentioning

confidence: 99%

Cytogenetical studies in five Atlantic Anguilliformes fishes

Vasconcelos

Molina

2009

Genet. Mol. Biol.

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The order Anguilliformes comprises 15 families, 141 genera and 791 fish species. Eight families had at least one karyotyped species, with a prevalence of 2n = 38 chromosomes and high fundamental numbers (FN). The only exception to this pattern is the family Muraenidae, in which the eight species analyzed presented 2n = 42 chromosomes. Despite of the large number of Anguilliformes species, karyotypic reports are available for only a few representatives. In the present work, a species of Ophichthidae, Myrichthys ocellatus (2n = 38; 8m+14sm+10st+6a; FN = 70) and four species of Muraenidae, Enchelycore nigricans (2n = 42; 6m+8sm+12st+16a; FN = 68), Gymnothorax miliaris (2n = 42; 14m+18sm+10st; FN = 84), G. vicinus (2n = 42; 8m+6sm+28a; FN = 56) and Muraena pavonina (2n = 42; 6m+4sm+32a; FN = 52), collected along the Northeastern coast of Brazil and around the St Peter and St Paul Archipelago were analyzed. Typical large metacentric chromosomes were observed in all species. Conspicuous polymorphic heterochromatic regions were observed at the centromeres of most chromosomes and at single ribosomal sites. The data obtained for Ophichthidae corroborate the hypothesis of a karyotypic diversification mainly due to pericentric inversions and Robertsonian rearrangements, while the identification of constant chromosome numbers in Muraenidae (2n = 42) suggests a karyotype diversification through pericentric inversions and heterochromatin processes.

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Section: Discussionmentioning

confidence: 99%

Cytogenetical studies in five Atlantic Anguilliformes fishes

Vasconcelos

Molina

2009

Genet. Mol. Biol.

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“…However, in the case of some chromosomes, reduced p arms are darkly stained after FPG staining showing their early replicating character. As GC-rich chromatin is thought to replicate early during S phase and AT-rich chro-matin clusters are rather late replicating, some of the platyfish subtelomeric regions can be considered as constituting GC-rich clusters other than NORs, which in fish are heterochromatic and seem to replicate at the end of the S phase (AMORES et al 1995;JANKUN et al 1998;BORON 2003). The lack of either the early or late replicating pattern in pericentromeric and telomeric heterochromatin is further proof of the heterogeneity of this component in the platyfish genome.…”

Section: Resultsmentioning

confidence: 99%

Identification of Early and Late Replicating Heterochromatic Regions on Platyfish (Xiphophorus maculatus) Chromosomes

Ocalewicz

2005

folia biol (krakow)

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The platyfish (Xiphophorus maculates) is one of the best studied lower vertebrates, however cytogenetic data on this species is rather limited. This paper presents advances in platyfish cytogenetics, performed by classical techniques enabling the identification of heterochromatin as well as early and late replicating chromosomal regions. Analysis of chromatin resistant to restriction enzymes (Alu I, Dde I and Hinf I) showed pericentromeric, telomeric and interstitial blocks of heterochromatin. DAPI fluorochrome staining revealed that some of the pericentromeric bands are discrete AT rich clusters of chromatin. Analysis of platyfish DNA replication on the chromosomal level showed heterogeneity of fish heterochromatin regarding its replication time; moreover, the replication banding pattern distinguished pairs of homologous chromosomes.

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“…Abbreviations: +, positivity; 2, negativity; /, no data available; A, terminal in the short arm of a middle submetacentric; B, proximal in a large/middle acrocentric; C, the entire short arm of a small/middle acro-subtelocentric; D, centromeric in a small acrocentric; E, proximal in the long arm of a large submetacentric. References: 1, reviewed in Salvadori et al (1997); 2, Takai et al (1987); 3, Salvadori et al (1994); 4, reviewed in Klinkhardt et al (1995); 5, Deiana et al (1990); 6, Coluccia et al (2001); 7, Cau et al (1988); 8, Pichiri et al (1995); 9, Salvadori et al (1995); 10, Amores et al (1995); 11, Thode (et al) 1985.…”

Section: Discussionmentioning

confidence: 97%

“…This is mainly due to the general difficulty in obtaining structural G, R, and Q bandings that enable karyotyping in mammals (Hellmer et al 1991). Replication banding is the most effective and repetitive method for obtaining serial bands in fish, but due to technical constraints, it has been applied in only a few fish species (Almeida Toledo et al 1988;Hellmer et al 1991;Amores et al 1995;Viñ as et al 1996;Salvadori et al 2003).…”

Section: Introductionmentioning

confidence: 98%

Colocalization of the ribosomal gene families inConger conger(Anguilliformes, Congridae)

Deiana

Coluccia

Cannas

et al. 2006

Italian Journal of Zoology

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The location of the major and minor ribosomal gene families in the conger eel (Conger conger) was investigated by in situ hybridization and CMA 3 staining. The two gene families were localized in only one chromosome pair, on the short arm of pair 19, in an entirely CMA 3 -positive region. Among the Anguilliformes, C. conger is the only species that shows the presence of the two gene families in the same chromosomal region.

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Replication, C and Ag-NOR chromosome-banding in two anguilliform fish species

Cited by 12 publications

References 22 publications

Cytogenetical studies in five Atlantic Anguilliformes fishes

Cytogenetical studies in five Atlantic Anguilliformes fishes

Identification of Early and Late Replicating Heterochromatic Regions on Platyfish (Xiphophorus maculatus) Chromosomes

Colocalization of the ribosomal gene families inConger conger(Anguilliformes, Congridae)

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