Replacing water and nutrients for ethanol production by ARTP derived biogas slurry tolerant Zymomonas mobilis strain

Duan, Guowei; Wu, Bo; Han, Qianqian; Wang, Wei‐Ting; Tan, Qiong; Dai, Yonghua; Qin, Yao; Tan, Furong; Hu, Guangyuan; He, Mingxiong

doi:10.1186/s13068-019-1463-2

Cited by 18 publications

(9 citation statements)

References 43 publications

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“…The highest enzyme activity of the mutant was 9.46(mutant 202(1)), 2.49 folds that of the original strain. ARTP mutagenesis has been widely used in the screening of yeast and lamentous fungi, and the desired results have been obtained (24,25). There were some studies that use ARTP mutagenesis to screen of Bacillus, which was 1.2-3.3 folds higher than the original strain (26,27).…”

Section: Artp Mutagenesis and Screening Of Mutantmentioning

confidence: 99%

Increasing chitosanase production in Bacilluse cereus by a novel mutagenesis and screeen method

Zhang

Zhao

2020

Preprint

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Background Chitosan hydrolysis by chitosanase is one of the most effective methods to produce chitosan oligosaccharides, however, the low yield of chitosanase cannot meet the current requirement. In this paper, a strain producing chitosanase was screened, and a novel mutagenesis system (1)) was selected to increase the yield of chitosanase. The hydrolyzed products from chitosan by chitosanase produced by mutan train were also analyzed by LC-MS. Results A strain of Bacillus cereus capable of producing chitosanase was screened and identified from soil samples. A mutant strain of Bacillus cereus was obtained by ARTP mutagenesis and bioscreening method, and chitosanase activity was 2.49 folds that of the original bacteria. After optimized fermentation conditions, the enzyme activity of the mutant strain was 3.3 folds that of the original bacteria. The relative molecular weight of the purified chitosanase was 43 kDa. Ten chitosan oligosaccharides(2–4 oligosaccharides) were obtained by hydrolyzing chitosan with it. Conclutions: The results showed that the ARTP mutagenesis and bioscreening method could significantly increase the yield of chitosanase in B. cereus, and had little effect on the properties of the enzyme. These findings have potential applications in mutagenesis of other enzyme-producing microorganisms.

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Section: Artp Mutagenesis and Screening Of Mutantmentioning

confidence: 99%

Increasing chitosanase production in Bacilluse cereus by a novel mutagenesis and screeen method

Zhang

Zhao

2020

Preprint

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“…With all nutrients needed for cell growth in the culture medium including a nitrogen source from YE, cell growth of Z. mobilis ZM4 is generally expected to be coupled with its glucose consumption and ethanol production. However, results and literature reports in a few studies from different research groups indicated that cell growth of Z. mobilis ZM4 and its glucose consumption were uncoupled, glucose was not completely consumed for ethanol production when cells reached stationary phase ( Jones and Doelle, 1991 ; He et al, 2012 ; Yang et al, 2014a ; Xia et al, 2018 ; Duan et al, 2019 ). This uncoupling phenomenon between growth and fermentation performance has also been reported in other microorganisms, including yeast, resulting in a prolonged fermentation time and decreased ethanol productivity ( Cot et al, 2007 ; Pagliardini et al, 2013 ).…”

Section: Introductionmentioning

confidence: 97%

The Magnesium Concentration in Yeast Extracts Is a Major Determinant Affecting Ethanol Fermentation Performance of Zymomonas mobilis

Jin

et al. 2020

Front. Bioeng. Biotechnol.

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Zymomonas mobilis is a model ethanologenic bacterium for diverse biochemical production. Rich medium (RM) is a complex medium that is routinely used to cultivate Z. mobilis, which contains carbon sources such as glucose, nitrogen sources such as yeast extract (YE), and KH 2 PO 4. Glucose consumption and cell growth of Z. mobilis is usually coupled during ethanol fermentation. However, sometimes glucose was not consumed during the exponential growth phase, and it took extended time for cells to consume glucose and produce ethanol, which eventually reduced the ethanol productivity. In this study, the effects of different nitrogen sources, as well as the supplementation of an additional nitrogen source into RM and minimal medium (MM), on cell growth and glucose consumption of Z. mobilis were investigated to understand the uncoupled cell growth and glucose consumption. Our results indicated that nitrogen sources such as YE from different companies affected cell growth, glucose utilization, and ethanol production. We also quantified the concentrations of major ion elements in different nitrogen sources using the quantitative analytic approach of Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES), and demonstrated that magnesium ion in the media affected cell growth, glucose consumption, and ethanol production. The effect of magnesium on gene expression was further investigated using RNA-Seq transcriptomics. Our results indicated that the lack of Mg 2+ triggered stress responses, and the expression of genes involved in energy metabolism was reduced. Our work thus demonstrated that Mg 2+ concentration in nitrogen sources is essential for vigorous cell growth and ethanol fermentation, and the difference of Mg 2+ concentration in different YE is one of the major factors affecting the coupled cell growth, glucose consumption and ethanol fermentation in Z. mobilis. We also revealed that genes responsive for Mg 2+ deficiency in the medium were majorly related to stress

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“…To overcome critical steps of microbial fermentation processes and to increase yields, technological advances are necessary on synthetic biology tools like metagenomics [20], genetic engineering [21], orthogonal communication systems [22], metaproteomics [23], metabolomics [24], and metabolic engineering [25][26][27].…”

Section: Introductionmentioning

confidence: 99%

Technological Advances in Synthetic Biology for Cellulosic Ethanol Production

Fantinel¹,

Margis²,

Talamini³

et al. 2022

Biorefineries - Selected Processes

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The resurgence of biofuels in the recent past has brought new perspectives for renewable energy sources. Gradually the optimistic scenarios were being challenged by the competition for raw materials dedicated to direct or indirect human food. Second-generation biorefineries have emerged as technological alternatives to produce biofuels from lignocellulosic biomass. The third generation of biorefineries uses alternative raw materials like algae and microalgae. Despite the technical feasibility, these biorefineries were indebted for their economic performance. Synthetic biology has provided new microbial platforms that are increasingly better adapted to industrial characteristics to produce biofuels and fine chemicals. Synthetic biology bioengineers microorganisms to take advantage of the low-cost and less-noble raw materials like lignocellulosic biomass, carbon dioxide, and waste as a sustainable alternative for bioenergy generation using bio-substrates. In this chapter, we analyze the innovations in synthetic biology as applied to cellulosic ethanol production based on registered patents issued over the last twenty years (1999–2019). Using Questel-Orbit Intelligence, we recovered a total of 298 patent families, from which we extracted the key concepts and technology clusters, the primary technological domains and applications, the geographical distribution of patents, and the leading patents assignees. Besides, we discuss the perspectives for future research and innovations and the market and policy opportunities for innovation in this technological field. We conclude that the patented technologies serve as a proxy for the development of synthetic biotechnology applied in cellulosic ethanol production by the fourth generation of biorefineries.

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Replacing water and nutrients for ethanol production by ARTP derived biogas slurry tolerant Zymomonas mobilis strain

Cited by 18 publications

References 43 publications

Increasing chitosanase production in Bacilluse cereus by a novel mutagenesis and screeen method

Increasing chitosanase production in Bacilluse cereus by a novel mutagenesis and screeen method

The Magnesium Concentration in Yeast Extracts Is a Major Determinant Affecting Ethanol Fermentation Performance of Zymomonas mobilis

Technological Advances in Synthetic Biology for Cellulosic Ethanol Production

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