Replacement of Animal Model for Propagation of Classical Swine Fever Challenge Virus by Adaption in the PK-15 Cell Line

Badasara, Surendra Kumar; Mohan, M.; Sah, V. K.; Kumari, Poonam; Upmanyu, Vikramaditya; Dhar, P.; Tiwari, Ashok Kumar; Chander, Vikas; Gupta, Vivek Kumar

doi:10.5958/2277-940x.2017.00087.0

Cited by 3 publications

(4 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Both the animals had marked leukopenia such as 1400 cells/cu.mm in one and 2950 cell/cu.mm in the second one, which is most pathognomic for CSF infection. Postmortem examination of these animals were also suggestive of CSF infection such as pinpoint hemorrhages on the kidneys (turkey egg appearance), hemorrhages on the tonsils, necrotic lesions in the intestine and necrosis of mesenteric and other lymph glands (Badasara et al, 2017). The blood collected at viraemia stage were also positive in CSF specific PCR (Fig.…”

Section: Correlation Of Mutations In the E Rns Gene Of Csfv With Virus Attenuationmentioning

confidence: 90%

“…The 681bp nucleotide sequence of the E rns gene of the live attenuated CSF cell culture vaccine of Indian origin (IVRI-CSF-BS) has been submitted in GenBank (Accession number MT424777). Upon analysis of the sequence, we observed only three nucleotide changes in the E rns gene in the vaccine virus compared to its parental virulent virus (Badasara et al, 2017) at passage 6 in cell culture.…”

Section: Sequencing Of E Rns Gene Of Csf Cell Culture Vaccine and Its Back Passagesmentioning

confidence: 92%

See 1 more Smart Citation

Sequencing of Erns Gene of a Live Attenuated Classical Swine Fever Cell Culture Vaccine Virus and its Comparison with Back Passages and Other CSFV Strains

Ompreethi¹,

Mitra²,

Pachauri³

et al. 2020

Int.J.Curr.Microbiol.App.Sci

View full text Add to dashboard Cite

Section: Correlation Of Mutations In the E Rns Gene Of Csfv With Virus Attenuationmentioning

confidence: 90%

Section: Sequencing Of E Rns Gene Of Csf Cell Culture Vaccine and Its Back Passagesmentioning

confidence: 92%

Sequencing of Erns Gene of a Live Attenuated Classical Swine Fever Cell Culture Vaccine Virus and its Comparison with Back Passages and Other CSFV Strains

Ompreethi¹,

Mitra²,

Pachauri³

et al. 2020

Int.J.Curr.Microbiol.App.Sci

View full text Add to dashboard Cite

“…Previously, intestines from experimentally infected pigs had been used to assess inactivation protocols [237]. Cell culture methods have been developed to eliminate the need for using pigs to produce virus for challenge stocks [238]. BALB/c mice have been used to study vaccines against CSFV [239].…”

Section: Classical Swine Fevermentioning

confidence: 99%

Transboundary Animal Diseases, an Overview of 17 Diseases with Potential for Global Spread and Serious Consequences

Clemmons

Alfson

Dutton

2021

Animals

View full text Add to dashboard Cite

Animals provide food and other critical resources to most of the global population. As such, diseases of animals can cause dire consequences, especially disease with high rates of morbidity or mortality. Transboundary animal diseases (TADs) are highly contagious or transmissible, epidemic diseases, with the potential to spread rapidly across the globe and the potential to cause substantial socioeconomic and public health consequences. Transboundary animal diseases can threaten the global food supply, reduce the availability of non-food animal products, or cause the loss of human productivity or life. Further, TADs result in socioeconomic consequences from costs of control or preventative measures, and from trade restrictions. A greater understanding of the transmission, spread, and pathogenesis of these diseases is required. Further work is also needed to improve the efficacy and cost of both diagnostics and vaccines. This review aims to give a broad overview of 17 TADs, providing researchers and veterinarians with a current, succinct resource of salient details regarding these significant diseases. For each disease, we provide a synopsis of the disease and its status, species and geographic areas affected, a summary of in vitro or in vivo research models, and when available, information regarding prevention or treatment.

show abstract

“…In India, live attenuated lapinized CSF vaccine is used to control CSF which is not freely available. Cell line based vaccines are considered to be always advantageous since these vaccines will be easy to scale up, devoid of the adventitious agents and comparatively cheaper, and attempts have been made to adapt CSFV to in vitro cultured cells (Dhar et al r 2008, Medhi et al 2012, Badasara et al 2017, Vadivoo et al 2017. The present study describes the adaptability of a virulent, field isolate of CSFV in PK-15 cell line.…”

mentioning

confidence: 99%

Adaptability of a field isolate of Classical swine fever virus to PK-15 cells

AKSHAYA

Ramesh

LOGESH

et al. 2020

Indian J of Anim Sci

View full text Add to dashboard Cite

Classical swine fever (CSF) is an economically important pig disease affecting rural pig farming and vaccines are not freely available for control. In the present study an Indian isolate of CSF virus was passaged 75 times in PK-15 cells. At different passages, presence of virus was confirmed by Fluorescent Antibody Virus Neutralization (FAVN) test and Reverse Transcription-Polymerase Chain Reaction (RTPCR) for NS5B, E2 and 5-UTR genes. TCID50 titers were found to range between 4.00 and 8.97 at 10th and 75th passages respectively. Back passage and pathogenicity studies in susceptible pigs, the natural host, indicated that the virus was found to be attenuated following PK-15 passages and did not produce any clinical signs after 45th and 75th passages.

show abstract

Replacement of Animal Model for Propagation of Classical Swine Fever Challenge Virus by Adaption in the PK-15 Cell Line

Cited by 3 publications

References 0 publications

Sequencing of Erns Gene of a Live Attenuated Classical Swine Fever Cell Culture Vaccine Virus and its Comparison with Back Passages and Other CSFV Strains

Sequencing of Erns Gene of a Live Attenuated Classical Swine Fever Cell Culture Vaccine Virus and its Comparison with Back Passages and Other CSFV Strains

Transboundary Animal Diseases, an Overview of 17 Diseases with Potential for Global Spread and Serious Consequences

Adaptability of a field isolate of Classical swine fever virus to PK-15 cells

Contact Info

Product

Resources

About