2017
DOI: 10.5958/2277-940x.2017.00087.0
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Replacement of Animal Model for Propagation of Classical Swine Fever Challenge Virus by Adaption in the PK-15 Cell Line

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Cited by 3 publications
(4 citation statements)
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“…Both the animals had marked leukopenia such as 1400 cells/cu.mm in one and 2950 cell/cu.mm in the second one, which is most pathognomic for CSF infection. Postmortem examination of these animals were also suggestive of CSF infection such as pinpoint hemorrhages on the kidneys (turkey egg appearance), hemorrhages on the tonsils, necrotic lesions in the intestine and necrosis of mesenteric and other lymph glands (Badasara et al, 2017). The blood collected at viraemia stage were also positive in CSF specific PCR (Fig.…”
Section: Correlation Of Mutations In the E Rns Gene Of Csfv With Virus Attenuationmentioning
confidence: 90%
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“…Both the animals had marked leukopenia such as 1400 cells/cu.mm in one and 2950 cell/cu.mm in the second one, which is most pathognomic for CSF infection. Postmortem examination of these animals were also suggestive of CSF infection such as pinpoint hemorrhages on the kidneys (turkey egg appearance), hemorrhages on the tonsils, necrotic lesions in the intestine and necrosis of mesenteric and other lymph glands (Badasara et al, 2017). The blood collected at viraemia stage were also positive in CSF specific PCR (Fig.…”
Section: Correlation Of Mutations In the E Rns Gene Of Csfv With Virus Attenuationmentioning
confidence: 90%
“…The 681bp nucleotide sequence of the E rns gene of the live attenuated CSF cell culture vaccine of Indian origin (IVRI-CSF-BS) has been submitted in GenBank (Accession number MT424777). Upon analysis of the sequence, we observed only three nucleotide changes in the E rns gene in the vaccine virus compared to its parental virulent virus (Badasara et al, 2017) at passage 6 in cell culture.…”
Section: Sequencing Of E Rns Gene Of Csf Cell Culture Vaccine and Its Back Passagesmentioning
confidence: 92%
“…Previously, intestines from experimentally infected pigs had been used to assess inactivation protocols [237]. Cell culture methods have been developed to eliminate the need for using pigs to produce virus for challenge stocks [238]. BALB/c mice have been used to study vaccines against CSFV [239].…”
Section: Classical Swine Fevermentioning
confidence: 99%
“…In India, live attenuated lapinized CSF vaccine is used to control CSF which is not freely available. Cell line based vaccines are considered to be always advantageous since these vaccines will be easy to scale up, devoid of the adventitious agents and comparatively cheaper, and attempts have been made to adapt CSFV to in vitro cultured cells (Dhar et al r 2008, Medhi et al 2012, Badasara et al 2017, Vadivoo et al 2017. The present study describes the adaptability of a virulent, field isolate of CSFV in PK-15 cell line.…”
mentioning
confidence: 99%