Repellents Inhibit P450 Enzymes in Stegomyia (Aedes) aegypti

Jaramillo, Gloria Isabel; Logan, James G.; Loza-Reyes, Elisa; Stashenko, Elena E.; Moores, G. D.

doi:10.1371/journal.pone.0048698

Cited by 26 publications

(13 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Such activity has been reported in EOs from sesame, Amyris , sandalwood, Helichrysum , cedar wood, and black pepper that exhibited synergistic effects in Aedes aegypti (Diptera: Culicidae) larvae while inhibiting cytochrome P450 monooxygenases and/or carboxylesterases in vitro [ 16 ]. Reduced esterase, glutathione-S-transferase, and/or monooxygenase activity following exposure to various EOs was similarly reported in other pests [ 46 – 49 ], and probably accounts for synergism of imidacloprid in several of our bioassays. This is significant given reports of M .…”

Section: Discussionsupporting

confidence: 78%

Plant Essential Oils Synergize and Antagonize Toxicity of Different Conventional Insecticides against Myzus persicae (Hemiptera: Aphididae)

2015

View full text Add to dashboard Cite

Plant-derived products can play an important role in pest management programs. Essential oils from Lavandula angustifolia (lavender) and Thymus vulgaris (thyme) and their main constituents, linalool and thymol, respectively, were evaluated for insecticidal activity and synergistic action in combination with insecticides against green peach aphid, Myzus persicae (Sulzer) (Hemiptera: Aphididae). The essential oils and their main constituents exerted similar insecticidal activity when aphids were exposed by direct sprays, but were non-toxic by exposure to treated leaf discs. In synergism experiments, the toxicity of imidacloprid was synergized 16- to 20-fold by L. angustifolia and T. vulgaris essential oils, but far less synergism occurred with linalool and thymol, indicating that secondary constituents of the oils were probably responsible for the observed synergism. In contrast to results with imidacloprid, the insecticidal activity of spirotetramat was antagonized by L. angustifolia and T. vulgaris essential oils, and linalool and thymol. Our results demonstrate the potential of plant essential oils as synergists of insecticides, but show that antagonistic action against certain insecticides may occur.

show abstract

Section: Discussionsupporting

confidence: 78%

Plant Essential Oils Synergize and Antagonize Toxicity of Different Conventional Insecticides against Myzus persicae (Hemiptera: Aphididae)

2015

View full text Add to dashboard Cite

show abstract

“…AChE and MFO activity in all of the treated Ae. aegypti strains in this study reduced when compared to the controls, as recorded after exposure to P. crispum essential oil for 24 h. Similar results of reduced activity of AChE [37,90,91] and MFO [22,26,92,93,94] were observed in several insect species exposed to different botanical products. These findings were contrary to those in many studies, which demonstrated biochemical responses with elevation of AChE or MFO activity after treatment with either synthetic substances or plant products.…”

Section: Discussionsupporting

confidence: 87%

Biochemical Effects of Petroselinum crispum (Umbellifereae) Essential Oil on the Pyrethroid Resistant Strains of Aedes aegypti (Diptera: Culicidae)

Intirach

Junkum

Lumjuan

et al. 2018

Insects

View full text Add to dashboard Cite

In ongoing screening research for edible plants, Petroselinum crispum essential oil was considered as a potential bioinsecticide with proven antimosquito activity against both the pyrethroid susceptible and resistant strains of Aedes aegypti. Due to the comparative mosquitocidal efficacy on these mosquitoes, this plant essential oil is promoted as an attractive candidate for further study in monitoring resistance of mosquito vectors. Therefore, the aim of this study was to evaluate the impact of P. crispum essential oil on the biochemical characteristics of the target mosquito larvae of Ae. aegypti, by determining quantitative changes of key enzymes responsible for xenobiotic detoxification, including glutathione-S-transferases (GSTs), α- and β-esterases (α-/β-ESTs), acetylcholinesterase (AChE), acid and alkaline phosphatases (ACP and ALP) and mixed-function oxidases (MFO). Three populations of Ae. aegypti, comprising the pyrethroid susceptible Muang Chiang Mai-susceptible (MCM-S) strain and the pyrethroid resistant Pang Mai Dang-resistant (PMD-R) and Upakut-resistant (UPK-R) strains, were used as test organisms. Biochemical study of Ae. aegypti larvae prior to treatment with P. crispum essential oil revealed that apart from AChE, the baseline activity of most defensive enzymes, such as GSTs, α-/β-ESTs, ACP, ALP and MFO, in resistant UPK-R or PMD-R, was higher than that determined in susceptible MCM-S. However, after 24-h exposure to P. crispum essential oil, the pyrethroid susceptible and resistant Ae. aegypti showed similarity in biochemical features, with alterations of enzyme activity in the treated larvae, as compared to the controls. An increase in the activity levels of GSTs, α-/β-ESTs, ACP and ALP was recorded in all strains of P. crispum oil-treated Ae. aegypti larvae, whereas MFO and AChE activity in these mosquitoes was decreased. The recognizable larvicidal capability on pyrethroid resistant Ae. aegypti, and the inhibitory effect on AChE and MFO, emphasized the potential of P. crispum essential oil as an attractive alternative application for management of mosquito resistance in current and future control programs.

show abstract

“…In a study by Joffe and colleagues, parsley seed oil and dillapiole oil where found to inhibit cytochrome P450 activity, but not esterase activity, providing a synergistic effect for pyrethrum on Musca domestica [ 108 ]. In mosquitos, several groups have reported on PDPs and inhibition: plant flavanoids are potent inhibitors of CYP6Z2; thymol and eugenol appear to inhibit both cytochrome P450 and Glutatione S-transferase activities; and essential oils of several native Columbian plants that possess repellent activity are inhibitors of cytochrome P450 activity [ 115 - 117 ].…”

Section: Reviewmentioning

confidence: 99%

Present and future potential of plant-derived products to control arthropods of veterinary and medical significance

et al. 2014

View full text Add to dashboard Cite

The use of synthetic pesticides and repellents to target pests of veterinary and medical significance is becoming increasingly problematic. One alternative approach employs the bioactive attributes of plant-derived products (PDPs). These are particularly attractive on the grounds of low mammalian toxicity, short environmental persistence and complex chemistries that should limit development of pest resistance against them.Several pesticides and repellents based on PDPs are already available, and in some cases widely utilised, in modern pest management. Many more have a long history of traditional use in poorer areas of the globe where access to synthetic pesticides is often limited. Preliminary studies support that PDPs could be more widely used to target numerous medical and veterinary pests, with modes of action often specific to invertebrates.Though their current and future potential appears significant, development and deployment of PDPs to target veterinary and medical pests is not without issue. Variable efficacy is widely recognised as a restraint to PDPs for pest control. Identifying and developing natural bioactive PDP components in place of chemically less-stable raw or 'whole’ products seems to be the most popular solution to this problem. A limited residual activity, often due to photosensitivity or high volatility, is a further drawback in some cases (though potentially advantageous in others). Nevertheless, encapsulation technologies and other slow-release mechanisms offer strong potential to improve residual activity where needed.The current review provides a summary of existing use and future potential of PDPs against ectoparasites of veterinary and medical significance. Four main types of PDP are considered (pyrethrum, neem, essential oils and plant extracts) for their pesticidal, growth regulating and repellent or deterrent properties. An overview of existing use and research for each is provided, with direction to more extensive reviews given in many sections. Sections to highlight potential issues, modes of action and emerging and future potential are also included.

show abstract

Repellents Inhibit P450 Enzymes in Stegomyia (Aedes) aegypti

Cited by 26 publications

References 33 publications

Plant Essential Oils Synergize and Antagonize Toxicity of Different Conventional Insecticides against Myzus persicae (Hemiptera: Aphididae)

Plant Essential Oils Synergize and Antagonize Toxicity of Different Conventional Insecticides against Myzus persicae (Hemiptera: Aphididae)

Biochemical Effects of Petroselinum crispum (Umbellifereae) Essential Oil on the Pyrethroid Resistant Strains of Aedes aegypti (Diptera: Culicidae)

Present and future potential of plant-derived products to control arthropods of veterinary and medical significance

Contact Info

Product

Resources

About