Repeated exposures to low-level chlorpyrifos results in impairments in sustained attention and increased impulsivity in rats

Abstract

Organophosphates such as chlorpyrifos (CPF) are among the most commonly used pesticides in the world. Therefore, it is not surprising that measurable levels of organophosphates (including CPF) are found in over 50% of fresh fruits, vegetables and grains that we consume and that approximately 80% of adults in the US have detectable levels of CPF metabolites in their urine. It is well known that acute exposure to organophosphates can cause cognitive deficits; however, the effects of daily or intermittent contact with low levels of organophosphates (often reflective of environmental exposures) are not well understood. The objective of this study was to determine if repeated lowlevel exposures to CPF impaired performance of the 5-Choice Serial Reaction Time Task (5C-SRTT), an animal model of sustained attention. Adult rats were trained to stably perform the 5C-SRTT, then treated with vehicle or CPF 18.0 mg/kg daily for 14 consecutive days or every other day for 30 days. Behavioral testing occurred daily during the CPF-exposure period and throughout a 30 day washout period to assess recovery. All CPF-treated animals exhibited deficits in percent correct, an increase in omissions and premature responses without signs impaired motivation or overt toxicity. Deficits in 5-CSRTT accuracy were apparent well into the 30 day washout period despite significant recovery of cholinesterase activity. These results indicate that repeated exposures to relatively low levels of chlorpyrifos lead to protracted impairments of sustained attention and an increase in impulsive behaviors in rats.

Repeated exposure to chlorpyrifos leads to prolonged impairments of axonal transport in the living rodent brain

“…The doses evaluated in this study were identified in previous studies (Terry et al, 2003(Terry et al, , 2011Middlemore-Risher et al, 2010) and operationally defined as doses not associated with acute signs of cholinergic toxicity (e.g., fasciculations, seizures, diarrhea, excessive urination, salivation, etc., see reviews, Rusyniak and Nanagas, 2004;Sungurtekin et al, 2006).…”

Toxicological studies for some agricultural waste extracts on mosquito larvae and experimental animals

“…Many studies indicate that repeated exposures to relatively low level of organophosphate pesticides such as chlorpyrifos, and monocrotophos lead to protected impairments of sustained attention and an increase in impulsive behaviors in rats [22,23] .…”

Organophosphorus Pesticides

“…When adult rats were treated orally with 18 mg/kg/day chlorpyrifos for 14 consecutive days or every other day for 30 days, they performed more poorly than controls on a test of sustained attention, the 5-Choice Serial Reaction Time Task (214).…”