Repeated exposure of mouse dermal fibroblasts at a sub-cytotoxic dose of UVB leads to premature senescence: A robust model of cellular photoaging

Zeng, Jiping; Bi, Bo; Chen, Liang; Yang, Ping; Guo, Yu; Zhou, Yiqun; Liu, Tianyi

doi:10.1016/j.jdermsci.2013.08.013

Cited by 36 publications

(36 citation statements)

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“…These effects of EEDO were mediated by inhibition of ROS generation, a key factor in premature senescence induced by UVB irradiation. 11,12) Treatment of EEDO to HR-1 hairless mice exposed to UVB significantly attenuated the number of senescent cells and reduced ROS levels in the skin.…”

Section: Discussionmentioning

confidence: 99%

“…2,10) Excess production and ineffective elimination of ROS production have thus been implicated in skin photoaging induced by UV radiation. 11,12) Dalbergia odorifera T. Chem (Leguminosae) is an indigenous medicinal herb that has been widely used in China and Korea for the treatment of ischemia, swelling, necrosis, rheumatic pain, and blood disorders. 13) Several components from the heartwood of D. odorifera, including flavonoids, quinines, and phenolic constituents, exhibit diverse biological activities.…”

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confidence: 99%

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Ethanol extract of Dalbergia odorifera protects skin keratinocytes against ultraviolet B-induced photoaging by suppressing production of reactive oxygen species

Ham

Hwang

Kang

et al. 2015

Bioscience, Biotechnology, and Biochemistry

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Ethanol extract of Dalbergia odorifera protects skin keratinocytes against ultraviolet B-induced photoaging by suppressing production of reactive oxygen species

Ham

Hwang

Kang

et al. 2015

Bioscience, Biotechnology, and Biochemistry

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“…At 12 h after plating, the cells were treated with RAPA in DMEM containing 10% FBS for 48 h; then, the medium was removed, and the cells were washed three times with phosphate-buffered saline (PBS). The cells were covered with a thin layer of PBS and exposed to a 4-dose series of UVB irradiation at 120 mJ/cm 2 (Narrowband TL 20 W/01 RS lamp, Philips, the Netherlands) for 120 s with an interval time of 12 h as previously described [30]. In this study, we present a model of stress-induced premature senescence (SIPS) through repeated exposure of MDFs at early passages (passages 1–3) to a series of 4 sub-cytotoxic doses of UVB (120 mJ/cm 2 ).…”

Section: Methodsmentioning

confidence: 99%

“…In this study, we present a model of stress-induced premature senescence (SIPS) through repeated exposure of MDFs at early passages (passages 1–3) to a series of 4 sub-cytotoxic doses of UVB (120 mJ/cm 2 ). Although the CCK-8 results showed that the cell viability was decreased by 30% after the series of 4 sub-cytotoxic doses of UVB (120 mJ/cm 2 ), the reduced viability did not affect our subsequent experiments [30]. After 4 UVB exposures, the cells were incubated for 24 h in DMEM with 10% FBS and then subjected to various biochemical investigations.…”

Section: Methodsmentioning

confidence: 99%

Rapamycin Protects Skin Fibroblasts from Ultraviolet B-Induced Photoaging by Suppressing the Production of Reactive Oxygen Species

Qin

Ren

Jia

et al. 2018

Cell Physiol Biochem

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Background/Aims: Ultraviolet B (UVB) irradiation alters multiple molecular pathways in the skin, thereby inducing skin photoaging. Murine dermal fibroblasts (MDFs) were subjected to a series of 4 sub-cytotoxic UVB doses (120 mJ/cm²), resulting in changes in cell shape, DNA damage, cell cycle arrest, extracellular matrix variations, reactive oxygen species (ROS) generation, and alterations in major intracellular antioxidant and cellular autophagy levels. Rapamycin (RAPA) is a new macrolide immunosuppressive agent that is primarily used in oncology, cardiology, and transplantation medicine and has been found to extend the lifespan of genetically heterogeneous mice. Several studies have shown that RAPA may have anti-aging effects in cells and organisms. Thus, in this study, we explored the effects and mechanisms of RAPA against the photoaging process using a well-established cellular photoaging model. Methods: We developed a stress-induced premature senescence (SIPS) model through repeated exposure of MDFs to ultraviolet B (UVB) irradiation. The cells were cultured in the absence or presence of RAPA for 48 h. Senescent phenotypes were assessed by examining cell viability, cell morphology, senescence-associated β-galactosidase (SA-β-gal) expression, cell cycle progression, intracellular ROS production, matrix metalloproteinase (MMP) synthesis and degradation, extracellular matrix (ECM) component protein expression, alterations in major intracellular antioxidant levels, and the cellular autophagy level. Results: Compared with the UVB group, pretreatment with RAPA (5 µM) significantly decreased the staining intensity and percentage of SA-β-gal-positive cells and preserved the elongated cell shape. Moreover, cells pretreated with RAPA showed inhibition of the reduction in the type I collagen content by blocking the UVB-induced upregulation of MMP expression. RAPA also decreased photoaging cell cycle arrest and downregulated p53 and p21 expression. RAPA application significantly attenuated irradiation-induced ROS release by modulating intracellular antioxidants and increasing the autophagy level. Conclusions: Our study demonstrated that RAPA elicited oxidative damage in vitro by reducing ROS accumulation in photoaged fibroblasts. The anti-aging effect can be attributed to the maintenance of normal antioxidant and cellular autophagy levels. However, determination of the definitive mechanism requires further study.

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“…Fatores intrínsecos e extrínsecos atuam na modificação de propriedades da pele no processo de envelhecimento cutâneo, podendo este ser, assim, classificado como envelhecimento intrínseco ou extrínseco (Gilchrest, 1989;Uitto e Bernstein, 1998 (Jenkins, 2002;Mine et al, 2008;Zouboulis e Makrantonaki, 2011;Raut et al, 2012 (Baumann, 2007;Tigges et al, 2014;Zeng et al, 2014).…”

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Avaliação da expressão gênica de colágeno em fibroblastos tratados com extratos de fungos endofíticos

Araújo¹

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Skin aging process is very complex and can be classified as intrinsic aging, primarily determined by genetic factors, and extrinsic aging, induced by nutritional and behavioral factors, such as chronic exposure to ultraviolet radiation, excessive alcohol consumption, smoking and nutritional disorders. Skin aging is characterized by morphological and functional changes, which can cause wrinkling, scaling, dryness, pigmentation abnormalities and skin cancer. Some of the disorders that affect the skin aging are mainly due to oxidative stress promoted by reactive oxygen species that are able to change the balance between synthesis and degradation of the dermal extracellular matrix components, favoring degradation rather than the synthesis process. Natural products protect skin from damage induced by oxidative stress and may act in important pathways in the skin aging. Among the possible sources for obtaining natural products it is possible to highlight the endophytic fungi, which are able to produce a great diversity of natural compounds. The objective of this study was to evaluate the influence of treatment with metabolites produced by two endophytic fungi isolated from Bauhinia variegate leaves on genic expression of collagen type I, metalloproteinase type 1 and tissue inhibitor of metalloproteinase type 1 in human dermal fibroblasts exposed to oxidative stress. The results show that both fungi were able to produce polyphenolic compounds and, more specifically, flavonoids. Treatment of fibroblasts with the extract produced by one of the endophytic fungi increased the expression of collagen type I gene, reduced the expression of metalloproteinase type 1 gene and inhibited metalloproteinase type 2 activity, suggesting that metabolites produced by this fungus can be benefic in prevent skin aging.

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Repeated exposure of mouse dermal fibroblasts at a sub-cytotoxic dose of UVB leads to premature senescence: A robust model of cellular photoaging

Cited by 36 publications

References 43 publications

Ethanol extract of Dalbergia odorifera protects skin keratinocytes against ultraviolet B-induced photoaging by suppressing production of reactive oxygen species

Ethanol extract of Dalbergia odorifera protects skin keratinocytes against ultraviolet B-induced photoaging by suppressing production of reactive oxygen species

Rapamycin Protects Skin Fibroblasts from Ultraviolet B-Induced Photoaging by Suppressing the Production of Reactive Oxygen Species

Avaliação da expressão gênica de colágeno em fibroblastos tratados com extratos de fungos endofíticos

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