Ethanol extract of <i>Dalbergia odorifera</i> protects skin keratinocytes against ultraviolet B-induced photoaging by suppressing production of reactive oxygen species

Ham, Sun Ah; Hwang, Jae Joon; Kang, Eun Sil; Yoo, Taesik; Lim, Hyun–Ho; Lee, Won Jin; Paek, Kyung Shin; Seo, Han Geuk

doi:10.1080/09168451.2014.993916

Cited by 26 publications

(14 citation statements)

References 30 publications

(37 reference statements)

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“…DOE was prepared according to a previously described method [26]. Briefly, heartwood of D. odorifera was purchased from a Korean medicinal herb store in Seoul in May 2012.…”

Section: Methodsmentioning

confidence: 99%

A Dalbergia odorifera extract improves the survival of endotoxemia model mice by inhibiting HMGB1 release

Choi

Park

Hwang

et al. 2017

BMC Complement Altern Med

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Background Dalbergia odorifera T. Chen (Leguminosae) is an indigenous medicinal herb that is widely used as a popular remedy in northern and eastern Asia. However, the cellular mechanisms underlying the biological activity of D. odorifera are not fully elucidated.MethodsAnti-inflammatory effect of D. odorifera extract (DOE) was determined through intraperitoneal injection in a mouse model of endotoxemia induced by lipopolysaccharide (LPS). RAW 264.7 cells, a murine macrophage, were also treated with LPS to generate a cellular model of inflammation, and investigated the anti-inflammatory activity and underlying mechanisms of DOE and its constituent isoliquiritigenin.ResultsDOE dose-dependently inhibited LPS-induced release of high mobility group box 1 (HMGB1), a late proinflammatory cytokine, and decreased cytosolic translocation of HMGB1 in RAW264.7 cells. This inhibitory effect of DOE on HMGB1 release was observed in cells treated with DOE before or after LPS treatment, suggesting that DOE is effective for both treatment and prevention. In addition, DOE significantly inhibited LPS-induced formation of nitric oxide (NO) and expression of inducible NO synthase (iNOS) in a dose-dependent manner. These effects of DOE were accompanied by suppression of HMGB1 release triggered by LPS, suggesting a possible mechanism by which DOE modulates HMGB1 release through NO signaling.Isoriquiritigenin, a constituent of DOE, also attenuated LPS-triggered NO formation and HMGB1 release in RAW264.7 cells, indicating that isoriquiritigenin is an indexing molecule for the anti-inflammatory properties of DOE. Furthermore, c-Jun N-terminal kinase, but not extracellular signal-regulated kinase and p38, mediated DOE-dependent inhibition of HMGB1 release and NO/iNOS induction in RAW 264.7 cells exposed to LPS. Notably, administration of DOE ameliorated survival rates in a mouse model of endotoxemia induced by LPS, where decreased level of circulating HMGB1 was observed.ConclusionThese results suggest that DOE confers resistance to LPS-triggered inflammation through NO-mediated inhibitory effects on HMGB1 release.

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“…DOE was prepared according to a previously described method [26]. Briefly, heartwood of D. odorifera was purchased from a Korean medicinal herb store in Seoul in May 2012.…”

Section: Methodsmentioning

confidence: 99%

A Dalbergia odorifera extract improves the survival of endotoxemia model mice by inhibiting HMGB1 release

Choi

Park

Hwang

et al. 2017

BMC Complement Altern Med

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“…Increasing evidence has indicated that ROS is one of the major causes of UVB-stimulated cellular senescence by damaging DNA strands and/or altering DNA bases [ 20 ]. To examine whether NF50E treatment could suppress UVB-induced cellular ROS production, a DCFDA-ROS detection assay was performed.…”

Section: Resultsmentioning

confidence: 99%

Protection against UVB-Induced Photoaging by Nypa fruticans via Inhibition of MAPK/AP-1/MMP-1 Signaling

Choi

Alam

Baek

et al. 2020

Oxidative Medicine and Cellular Longevity

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Ultraviolet B (UVB) irradiation is major causative factor in skin aging. The aim of the present study was to investigate the protective effect of a 50% ethanol extract from Nypa fruticans (NF50E) against UVB-induced skin aging. The results indicated that NF50E exerted potent antioxidant activity (IC50 = 17.55 ± 1.63 and 10.78 ± 0.63 μg/mL for DPPH and ABTS-radical scavenging activity, respectively) in a dose-dependent manner. High-performance liquid chromatography revealed that pengxianencin A, protocatechuic acid, catechin, chlorogenic acid, epicatechin, and kaempferol were components of the extract. In addition, the extract exhibited elastase inhibitory activity (IC50 = 17.96 ± 0.39 μg/mL). NF50E protected against UVB-induced HaCaT cell death and strongly suppressed UVB-stimulated cellular reactive oxygen species generation without cellular toxicity. Moreover, topical application of NF50E mitigated UVB-induced photoaging lesions including skin erythema and skin thickness in BALB/C mice. NF50E treatment inhibited UVB-induced collagen degradation as well as MMP-1 and IL-1β expressions and significantly stimulated SIRT1 expression. Furthermore, the extract treatment markedly suppressed the activation of NF-κB and AP-1 (p-c-Jun) by deactivating the p38 and JNK proteins. Taken together, current data suggest that NF50E exhibits potent antioxidant potential and protection against photoaging by attenuating MMP-1 activity and collagen degradation possibly through the downregulation of MAPK/NF-κB/AP-1 signaling and SIRT1 activation.

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“…Sativanone was reported to be distributed from the Restharrow root [ 35 ] and the heartwood of Dalbergia odorifera and showed several bioactivities including antibacterial activity against Ralstonia solanacearum [ 30 ] and anti-senescent and antioxidant effects [ 36 ]. This compound was also reported to possibly possess yeast α-glucosidase inhibition by using ultra-filtration liquid chromatography/mass spectrometry (UF-LC/MS) [ 37 ].…”

Section: Resultsmentioning

confidence: 99%

New Records of Potent In-Vitro Antidiabetic Properties of Dalbergia tonkinensis Heartwood and the Bioactivity-Guided Isolation of Active Compounds

et al. 2018

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Alpha-glucosidase inhibitory activity has been commonly used for the evaluation of antidiabetic property in vitro. The aim of this study is to investigate and characterize Dalbergia tonkinensis as a potential source of antidiabetic compounds. The screening of the active parts used, such as trunk bark, heartwood, and the leaves of Dalbergia tonkinensis indicated that all these extracted parts used with methanol demonstrated potent α-glucosidase inhibitory activity. The in vitro antidiabetic property of Dalbergia tonkinensis was notably recorded for the first time and showed activity (EC50 = 0.17–0.78 mg/mL) comparable to those of reported potent herbal extracts (EC50 = 0.25–4.0 mg/mL) and higher activity than that of acarbose, a commercial antidiabetic drug (EC50 = 1.21 mg/mL). The stability tests revealed that the heartwood of Dalbergia tonkinensis extract (HDT) possesses high pH stability with relative activity in the range of 80–98%. Further bioassay-guided purification led to the isolation of 2 active compounds identified as sativanone and formononetin from the ethyl acetate fraction and water fraction of HDT, respectively. These α-glucosidase inhibitors (aGIs) show promising inhibition against various types of α-glucosidases. Remarkably, these inhibitors were determined as new mammalian aGIs, showing good effect on rat α-glucosidase. The results suggest that Dalbergia tonkinensis is a potent source of aGIs and suggest promise in being developed as functional food with antidiabetic efficacy. The results of this study also enrich our knowledge concerning current biological activity and constituents of Dalbergia tonkinensis species.

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Ethanol extract of Dalbergia odorifera protects skin keratinocytes against ultraviolet B-induced photoaging by suppressing production of reactive oxygen species

Abstract: (2015) Ethanol extract of Dalbergiaodorifera protects skin keratinocytes against ultraviolet B-induced photoaging by suppressing production of reactive oxygen species, Bioscience, Biotechnology, and Biochemistry, 79:5,[760][761][762][763][764][765][766]

Cited by 26 publications

References 30 publications

A Dalbergia odorifera extract improves the survival of endotoxemia model mice by inhibiting HMGB1 release

A Dalbergia odorifera extract improves the survival of endotoxemia model mice by inhibiting HMGB1 release

Protection against UVB-Induced Photoaging by Nypa fruticans via Inhibition of MAPK/AP-1/MMP-1 Signaling

New Records of Potent In-Vitro Antidiabetic Properties of Dalbergia tonkinensis Heartwood and the Bioactivity-Guided Isolation of Active Compounds

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