Repartition effect of aromatic polyaniline coatings on the separation of bioactive peptides in capillary electrophoresis

Abstract

The capillary walls of fused-silica capillary electrophoresis (CE) columns were modified with a thin film of polyaniline (PANI), providing open-tubular columns with a stable coating containing aromatic groups and amine functionalities. Fast and efficient separations were observed for small bioactive peptides under acidic conditions on PANI-coated columns. The mechanism of separation is based on hydrophobic interactions between the analytes and the polymeric matrix. Good reproducibility was observed from run-to-run. Due to the simple derivatization procedure, method flexibility, the uniformity of the coating and its stability, conjugated polymers could find practical application in capillary zone electrophoresis (CZE) separations.

Properties of poly-aminophenylboronate coatings in capillary electrophoresis for the selective separation of diastereoisomers and glycoproteins

“…The same held true in case of polyaniline coatings for the separation of peptides [31]. The possible explanation is a mechanism of separation for the APBA polymer that acts through interactions between the analyte and the polymer coating, as already supposed in case of polyaniline-modified columns.…”

Protein adsorption under electrical stimulation of neural probe coated with polyaniline

“…This provided evidence of preferred FN and BSA protein adsorption on PANI films and that the nanosurface properties (i.e., greater surface nanoroughness with increased surface energy) of PANI films coating acted as a favorable environment for protein adsorption. Some investigations into electric potential assisted deposition of proteins reported that protein adsorption was usually spontaneous, and the electrostatic interactions naturally played an important role [28,29]. During the electrical stimulation, protein adsorption behavior could be expected to depend on the electrostatic interaction between the negatively charged proteins and the electrical field generated on the PANI films.…”

Current research activity in biosensors

“…Several aspects of current work on CE biosensors are summarized herein. An aromatic polyaniline-coated CE chip has been developed for separation of bioactive peptides [186] and a PPF-coated CE chip has been developed for the separation of protein, cytochrome c (pI 9.6), hemoglobin (pI 7.0), and phycocyanin (pI 4.7) [123]. Laserinduced fluorescence (LIF) is the most common detection system for microchips, because of its high sensitivity.…”