Repairing tRNA termini: News from the 3' end

Rammelt, Christiane; Rossmanith, Walter

doi:10.1080/15476286.2016.1239007

Cited by 9 publications

(7 citation statements)

References 82 publications

(101 reference statements)

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“…As far as we are aware, this is a novel cleavage specificity of an RNase acting on tRNA (Ogawa, 2016). Notably, a tRNA variant truncated at the 3 0 end by 4 nt cannot be repaired by host CCA-polymerase that requires the ''discriminatory'' nucleotide for adding a 3 0 tail (Rammelt and Rossmanith, 2016). This prevents further aminoacylation and inhibits translation, potentially leading to reduced cell metabolism or dormancy as a strategy for antiviral defense (Lopatina et al, 2020).…”

Section: Discussion Trna Cleavage Specificitymentioning

confidence: 99%

HEPN-MNT Toxin-Antitoxin System: The HEPN Ribonuclease Is Neutralized by OligoAMPylation

et al. 2020

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Section: Discussion Trna Cleavage Specificitymentioning

confidence: 99%

HEPN-MNT Toxin-Antitoxin System: The HEPN Ribonuclease Is Neutralized by OligoAMPylation

et al. 2020

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“… 41 , 42 Protein Rnt is an incumbent in tRNA 3′-end processing, which is very important for tRNA biogenesis for editing and repairing of tRNAs. 43 The nondetection of these proteins might indicate the defects in tRNA biogenesis of the bacteria, which might lead to significant problems in bacterial RNA metabolism, depending on the 3-HPAA exposure.…”

Section: Results and Discussionmentioning

confidence: 99%

“…aeruginosa . The geranylation provides increase in codon recognition fidelity and reduction in frameshift reading by taking action at the wobble position (U34) in the anticodon of lysine, glutamine, and glutamic acid. , Protein Rnt is an incumbent in tRNA 3′-end processing, which is very important for tRNA biogenesis for editing and repairing of tRNAs . The nondetection of these proteins might indicate the defects in tRNA biogenesis of the bacteria, which might lead to significant problems in bacterial RNA metabolism, depending on the 3-HPAA exposure.…”

Section: Results and Discussionmentioning

confidence: 99%

Pseudomonas aeruginosa Presents Multiple Vital Changes in Its Proteome in the Presence of 3-Hydroxyphenylacetic Acid, a Promising Antimicrobial Agent

Özdemir

Soyer

2020

ACS Omega

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Pseudomonas aeruginosa , a widely distributed opportunistic pathogen, is an important threat to human health for causing serious infections worldwide. Due to its antibiotic resistance and virulence factors, it is so difficult to combat this bacterium; thus, new antimicrobial agents are in search. 3-Hydroxyphenylacetic acid (3-HPAA), which is a phenolic acid mostly found in olive oil wastewater, can be a promising candidate with its dose-dependent antimicrobial properties. Elucidating the molecular mechanism of action is crucial for future examinations and the presentation of 3-HPAA as a new agent. In this study, the antimicrobial activity of 3-HPAA on P. aeruginosa and its action mechanism was investigated via shot-gun proteomics. The data, which are available via ProteomeXchange with identifier PXD016243, were examined by STRING analysis to determine the interaction networks of proteins. KEGG Pathway enrichment analysis via the DAVID bioinformatics tool was also performed to investigate the metabolic pathways that undetected and newly detected groups of the proteins. The results displayed remarkable changes after 3-HPAA exposure in the protein profile of P. aeruginosa related to DNA replication and repair, RNA modifications, ribosomes and proteins, cell envelope, oxidative stress, as well as nutrient availability. 3-HPAA showed its antimicrobial action on P. aeruginosa by affecting multiple bacterial processes; hence, it could be categorized as a multitarget antimicrobial agent.

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“…A similar restoration of incomplete genes occurs during maturation of tRNAs in the eukaryotic nucleus and many archaea and bacteria and involves addition of a CCA sequence at the transcript’s 3′ end (reviewed in 42 ). The completion of 3′ termini of several animal mitochondrial tRNAs by polyadenylation represents another well-known example of post-transcriptional ‘repair’ 43 , 44 . Lastly, in certain dinoflagellate mtDNAs, the gene cox3 is split into two fragments, which are brought together at the RNA level by an unknown mechanism 45 .…”

Section: Discussionmentioning

confidence: 99%

Keeping it complicated: Mitochondrial genome plasticity across diplonemids

Valach

Moreira

Hoffmann

et al. 2017

Sci Rep

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Chromosome rearrangements are important drivers in genome and gene evolution, with implications ranging from speciation to development to disease. In the flagellate Diplonema papillatum (Euglenozoa), mitochondrial genome rearrangements have resulted in nearly hundred chromosomes and a systematic dispersal of gene fragments across the multipartite genome. Maturation into functional RNAs involves separate transcription of gene pieces, joining of precursor RNAs via trans-splicing, and RNA editing by substitution and uridine additions both reconstituting crucial coding sequence. How widespread these unusual features are across diplonemids is unclear. We have analyzed the mitochondrial genomes and transcriptomes of four species from the Diplonema/Rhynchopus clade, revealing a considerable genomic plasticity. Although gene breakpoints, and thus the total number of gene pieces (~80), are essentially conserved across this group, the number of distinct chromosomes varies by a factor of two, with certain chromosomes combining up to eight unrelated gene fragments. Several internal protein-coding gene pieces overlap substantially, resulting, for example, in a stretch of 22 identical amino acids in cytochrome c oxidase subunit 1 and NADH dehydrogenase subunit 5. Finally, the variation of post-transcriptional editing patterns across diplonemids indicates compensation of two adverse trends: rapid sequence evolution and loss of genetic information through unequal chromosome segregation.

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Repairing tRNA termini: News from the 3' end

Cited by 9 publications

References 82 publications

HEPN-MNT Toxin-Antitoxin System: The HEPN Ribonuclease Is Neutralized by OligoAMPylation

HEPN-MNT Toxin-Antitoxin System: The HEPN Ribonuclease Is Neutralized by OligoAMPylation

Pseudomonas aeruginosa Presents Multiple Vital Changes in Its Proteome in the Presence of 3-Hydroxyphenylacetic Acid, a Promising Antimicrobial Agent

Keeping it complicated: Mitochondrial genome plasticity across diplonemids

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