Repairing of goat Tibial Bone Defects with BMP-2 Gene–Modified Tissue-Engineered Bone

Dai, Kerong; Xu, Xiaofeng; Tang, Tingting; Zhu, Zhenan; Yu, Chang; Lou, Jueren; Zhang, X. L.

doi:10.1007/s00223-004-0095-z

Cited by 112 publications

(85 citation statements)

References 26 publications

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“…[12][13][14][15][16][17][18][19][20][21] Despite their excellent biocompatibility, the osteoconductive characteristics of the in situ crosslinkable methacrylate-endcapped poly-(D,…”

Section: Resultsmentioning

confidence: 99%

“…The osteogenic potential of BMSCs has been demonstrated extensively both in vitro and in vivo, and many studies have succeeded in repairing bone defects using BMSCs in different animal models. [12][13][14][15][16][17][18][19][20][21] Not only can osteogenically induced BMSCs provide a source for new bone formation, but the transformed cells can also secrete important growth factors regulating further bone healing.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of an Injectable, Photopolymerizable, and Three-Dimensional Scaffold Based on Methacrylate-Endcapped Poly(D,L-Lactide-co-ɛ-Caprolactone) Combined with Autologous Mesenchymal Stem Cells in a Goat Tibial Unicortical Defect Model

Vertenten

Lippens

Gironès

et al. 2009

Tissue Engineering Part A

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An in situ crosslinkable, biodegradable, methacrylate-endcapped poly(D,L-lactide-co-e-caprolactone) in which crosslinkage is achieved by photoinitiators was developed for bone tissue regeneration. Different combinations of the polymer with bone marrow-derived mesenchymal stem cells (BMSCs) and a-tricalcium phosphate (a-TCP) were tested in a unicortical tibial defect model in eight goats. The polymers were randomly applied in one of three defects (6.0 mm diameter) using a fourth unfilled defect as control. Biocompatibility and bone-healing characteristics were evaluated by serial radiographies, histology, histomorphometry, and immunohistochemistry. The results demonstrated cell survival and proliferation in the polymer-substituted bone defects. The addition of a-TCP was associated with less expansion and growth of the BMSCs than other polymer composites.

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“…[12][13][14][15][16][17][18][19][20][21] Despite their excellent biocompatibility, the osteoconductive characteristics of the in situ crosslinkable methacrylate-endcapped poly-(D,…”

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Evaluation of an Injectable, Photopolymerizable, and Three-Dimensional Scaffold Based on Methacrylate-Endcapped Poly(D,L-Lactide-co-ɛ-Caprolactone) Combined with Autologous Mesenchymal Stem Cells in a Goat Tibial Unicortical Defect Model

Vertenten

Lippens

Gironès

et al. 2009

Tissue Engineering Part A

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“…In this study the effect of biocompatible carriers ACS and hydroxyapatite implants impregnated with bovine BMP was tested to demonstrate that native bovine BMP extract could be ostegenic and serve as graft substitute in the repair of large segmental bone defects in a large animal model. It has been reported that local delivery of BMP has disadvantages which includes short halflife, need for repeated application and large dose requirement resulting in high cost [11,15] In this study however high BMP cost was reduced by substitution of urea for guanidine hydrochloride (GuHcl) at the initial stage of extraction [16,17].…”

Section: Introductionmentioning

confidence: 87%

Use of Native Bovine Bone Morphogenetic Protein Extract in Healing Segmental Tibial Bone Defects in Goats

Uwagie¹,

Awasum²

2015

J Veterinar Sci Technol

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We tested the new bone-forming activity of injectable native bovine BMP extracted from cadaveric bones obtained from the abattoir after SDS PAGE electrophoresis was used to determine the molecular weights and extract was bio-assayed in the goat thigh muscle pouch model. The bovine BMP extract was further used as an implant in an absorbable collagen sponge (ACS) and hydroxyapatite to heal segmental bone defects in a large animal tibia fracture model. Open tibia fractures were created in 20 adult goats with loss of 1.5 cm segment of the bone. 10 goats were treated with an implant of the study device (0.2 mg of extracted bovine BMP, ACS and hydroxyapatite) while 10 goats were treated with an implant of Buffer, ACS and hydroxyapatite. The devices were implanted as a mould in the segmental defects. The animals were monitored for callus formation which was measured on lateral radiographs and mean callus indexes determined. Radiographs indicated increased callus at 3 weeks in the extracted bovine BMP/ACS/hydroxyapatite treated tibiae. At 6 weeks, the extracted bovine BMP/ACS/ hydroxyapatite treated tibiae had superior radiographic healing scores compared with the control group. The extracted bovine BMP/ACS/hydroxyapatite treated tibiae produced significantly larger volume of callus (p<0.02) compared to the buffer/ACS/hydroxyapatite treated tibiae. Total callus and new bone volume was significantly increased (p<0.02) in the extracted bovine BMP/ACS/hydroxyapatite treated tibiae compared with buffer/ACS/ hydroxyapatite groups. Extracted bovine BMP/ACS/hydroxyapatite altered the timing of onset of periosteal/ endosteal callus formation in the treated groups compared to untreated controls.

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“…Due to the short half-life and heterotopic ossification seen with locally administered BMP, studies have examined the use of gene therapy to increase BMP production at the site of bone defects and spinal fusions [15,[35][36][37]. In a goat model for ON, Tang and colleagues were able to show the formation of lamellar bone using a β-tricalcium phosphate (β-TCP) scaffold loaded with BMP-2 gene-modified MSCs [22].…”

Section: Animal Studies Investigating Bone Morphogenetic Proteins Formentioning

confidence: 99%

Osteonecrosis of the femoral head: treatment with ancillary growth factors

Houdek

Wyles

Sierra

2015

Curr Rev Musculoskelet Med

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Osteonecrosis (ON) of the femoral head, also known as avascular necrosis (AVN) of the femoral head, is a progressive disease that predominantly affects younger patients. During early stage of ON, decompression of the femoral head has been commonly used to improve pain. The decompression has been augmented with nonvascularized or vascularized bone grafts, mesenchymal stems cells, and growth factors. The use of adjuvant growth factors to supplement the core decompression has mainly been limited to animal models in an attempt to regenerate the necrotic lesion of ON. Factors utilized include bone morphogenetic proteins, vascular endothelial growth factors, hepatocyte growth factors, fibroblast growth factors, granulocyte colonystimulating factors, and stem cells factors. In animal models, the use of these factors has been shown to increase bone formation and angiogenesis. Although promising, the use of these growth factors and cell-based therapies clinically remains limited.

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Repairing of goat Tibial Bone Defects with BMP-2 Gene–Modified Tissue-Engineered Bone

Cited by 112 publications

References 26 publications

Evaluation of an Injectable, Photopolymerizable, and Three-Dimensional Scaffold Based on Methacrylate-Endcapped Poly(D,L-Lactide-co-ɛ-Caprolactone) Combined with Autologous Mesenchymal Stem Cells in a Goat Tibial Unicortical Defect Model

Evaluation of an Injectable, Photopolymerizable, and Three-Dimensional Scaffold Based on Methacrylate-Endcapped Poly(D,L-Lactide-co-ɛ-Caprolactone) Combined with Autologous Mesenchymal Stem Cells in a Goat Tibial Unicortical Defect Model

Use of Native Bovine Bone Morphogenetic Protein Extract in Healing Segmental Tibial Bone Defects in Goats

Osteonecrosis of the femoral head: treatment with ancillary growth factors

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