Evaluation of an Injectable, Photopolymerizable, and Three-Dimensional Scaffold Based on Methacrylate-Endcapped Poly(D,L-Lactide-co-ɛ-Caprolactone) Combined with Autologous Mesenchymal Stem Cells in a Goat Tibial Unicortical Defect Model

Abstract

An in situ crosslinkable, biodegradable, methacrylate-endcapped poly(D,L-lactide-co-e-caprolactone) in which crosslinkage is achieved by photoinitiators was developed for bone tissue regeneration. Different combinations of the polymer with bone marrow-derived mesenchymal stem cells (BMSCs) and a-tricalcium phosphate (a-TCP) were tested in a unicortical tibial defect model in eight goats. The polymers were randomly applied in one of three defects (6.0 mm diameter) using a fourth unfilled defect as control. Biocompatibility and bone-healing characteristics were evaluated by serial radiographies, histology, histomorphometry, and immunohistochemistry. The results demonstrated cell survival and proliferation in the polymer-substituted bone defects. The addition of a-TCP was associated with less expansion and growth of the BMSCs than other polymer composites.

Platelet-Rich Plasma Favors Proliferation of Canine Adipose-Derived Mesenchymal Stem Cells in Methacrylate-Endcapped Caprolactone Porous Scaffold Niches

“…CLMA generates scaffolds with controlled porosity for tissue engineering [18] that may favor the integration of stem cells in the damaged tissue that would allow bone regeneration after differentiation. In fact, cASCs are able to adhere to CLMA (Figure 3a–c) without additional modifications of CMLA.…”

“…Caprolactone 2-(methacryloyloxy) ethyl ester (CLMA), allows enhanced cellular adhesion and proliferation [17]. CLMA generates scaffolds with controlled porosity for tissue engineering [18] that may favor the integration of stem cells in the damaged tissue that after differentiation would allow sites for regeneration in osteoarticular –related pathologies. ASCs isolated under aseptic conditions and amplified in GMP clinical-compatible criteria allow for better translational applications.…”

Cell survival and proliferation after encapsulation in a chemically modified Pluronic® F127 hydrogel

“…However, the bone regeneration capacity was rather limited in these in vivo experiments. 2 The limited permeability of the used polyester polymer was most likely responsible for the hampered bone formation in the defect side. Therefore, the use of Plu ALA-L, an alternative glue, would first of all allow a homogenous distribution of cell loaded CultiSpher-S Õ carriers in the bony defect.…”

Electrospun Nanofibrous P(DLLA–CL) Balloons as Calcium Phosphate Cement Filled Containers for Bone Repair: in Vitro and in Vivo Studies

“…However, although systematic comparisons between the in vitro and in vivo behaviors of ENPBs are important before they are evaluated in the clinic, such comparisons were not made in our previous work Polymer materials have been widely used as biomedical materials. [27][28][29][30][31] Many synthetic polymer biomaterials similar to P(DLLA-CL), such as PDLLA, [32][33][34][35] PLGA, 36-37 P(LLA-CL), 38 and PCL, [39][40] have been assessed in vivo to determine their behaviors in the bone microenvironments of sheep, [32][33][35][36] rabbit, 34,37 and pig. [38][39][40] However, there have been no reports evaluating the in vivo behaviors of P(DLLA-CL) biomaterials in bone microenvironments thus far.…”