Repair of segmental radial defect with autologous bone marrow aspirate and hydroxyapatite in rabbit radius: A clinical and radiographic evaluation

Abstract

Aim:Finding an ideal bone substitute to treat large bone defects, delayed union and nonunions remain a challenge for orthopedic surgeons and researchers. Several studies have been conducted on bone regeneration; each has its own advantages and disadvantages. The aim of this study was to evaluate the effect of a combination of hydroxyapatite (HA) powder with autologous bone marrow (BM) aspirate on the repair of segmental radial defect in a rabbit model.Materials and Methods:A total of 36 male and adult New Zealand rabbit with a mean weight of 2.25 kg were used in this study. Approximately, 5 mm defect was created in the mid-shaft of the radius to be filled with HA powder in the control group “HA” (n=18) and with a combination of HA powder and autologous BM aspirate in the test group “HA+BM” (n=18). Animals were observed daily for healing by inspection of the surgical site, and six rabbits of each group were sacrificed at 30, 60, and 90 post-operative days to perform a radiographic evaluation of defect site.Results:Obtained results revealed a better and more rapid bone regeneration in the test group: Since the defect was rapidly and completely filled with mature bone tissue after 90 days.Conclusion:Based on these findings, we could infer that adding a BM aspirate to HA is responsible of a better regeneration process leading to a complete filling of the defect.

Comprehensive In Vitro Testing of Calcium Phosphate-Based Bioceramics with Orthopedic and Dentistry Applications

“…Long bone reconstruction uses a distraction osteogenesis model—a surgical model that creates a gap in a long bone (usually tibia), using continuous traction on the osteotomy ends. This model was used with various rodent species (e.g., mouse [184,185,186,187], rat [188,189,190,191], and rabbit [192,193,194]) and even larger mammalians (e.g., sheep [195,196] or goats [197]). Endo-osseous implants are used for dental reconstruction or oromaxilofacial reconstruction after tumor resection [198], but their biocompatibility also needs testing [199].…”

Effects of bone types, particle sizes, and gamma irradiation doses in feline demineralized freeze-dried bone allograft

“…However, the allograft technique also provides an excellent alternative to autograft. It has the advantages of higher availability in terms of quantity compared to autograft and lower rejection reaction compared to xenograft while also can be done in a single surgery procedure [ 3 , 5 , 6 ]. Allograft can also avoid sacrificing the normal structure of the patient’s anatomy, reduce the need for prolonged hospital care and cost, and it also allows manipulation of material’s size and shape [ 6 ].…”

“…Bone grafting is a bone replacing technique by donor bone or bone-like material in a gap or around the broken bone through the surgical procedure on a recipient that can stimulate bone healing processes [1,2]. There are several types of bone graft with their own advantages and disadvantages [3,4]. Autograft, isograft, allograft, and xenograft are well known in both human and veterinary medicine [1][2][3][5][6][7].…”

“…There are several types of bone graft with their own advantages and disadvantages [3,4]. Autograft, isograft, allograft, and xenograft are well known in both human and veterinary medicine [1][2][3][5][6][7]. Each type of available bone graft possesses a different level of osteogenic, osteoinductive, and osteoconductive properties [1,3,8].…”

Orthogonal test design for the optimization of superparamagnetic chitosan plasmid gelatin microspheres that promote vascularization of artificial bone

“…Large segmental bone defect is one of the major problems that require urgent clinical solution (Verrier, Alini, Alsberg, et al, ; Yassine, Mokhtar, Houari, Karim, & Mohamed, ). Tissue engineering is an effective method for bone restoration, and angiogenesis in tissue‐engineered bone is a key factor in the use of this technology in clinical applications (Almubarak et al, ; Fan, Crawford, & Xiao, ; Jin et al, ; Zhao et al, ).…”