Repair of Radiation-induced DNA Strand Breaks in Thymocytes

Gärtner, Ch.; Sexauer, C.; Hagen, U.

doi:10.1080/09553007714551021

Cited by 4 publications

(3 citation statements)

References 13 publications

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“…The rationale of the QPCR technique is that certain DNA lesions block the movement of the Taq polymerase on the DNA template, so decreasing its amplification [ 40 , 41 ]. In the present study, we do not state any direct relationship between repair and protection in β -globin gene in thymocytes and amelioration of adverse effects of radiation; however, the only rationale of studying β -globin gene in thymocytes is based on the fact that radiosensitive nature of thymocytes makes them a highly sensitive system to study DNA repair [ 26 , 27 ] and constitutive expression in transcriptionally active and inactive regions of β -globin makes it a suitable target gene [ 28 , 29 ]. Exposing the mice to 10 Gy resulted in extensive DNA damage in the β -globin gene and complete immediate loss of amplification after exposure (Figures 3(a) and 3(b) ).…”

Section: Discussionmentioning

confidence: 99%

“…The radiosensitive nature of thymocytes provides an attractive system to study DNA repair [26, 27]. As we are interested to look at the early kinetics of DNA repair within 1 h after irradiation, we choose mouse β -globin gene which is constitutively expressed in transcriptionally active or inactive domains thus acting as a biomarker of overall DNA repair [28, 29].…”

Section: Introductionmentioning

confidence: 99%

“…Intraperitoneal administration of REC-2006 to mice at 15 mg kg −1 body weight conferred more than 90% protection against whole-body irradiation (10 Gy) as compared to 72% offered by 34.5 mg kg −1 body weight administration of the parent plant extract RP-1. The radiosensitive nature of thymocytes provides an attractive system to study DNA repair [ 26 , 27 ]. As we are interested to look at the early kinetics of DNA repair within 1 h after irradiation, we choose mouse β -globin gene which is constitutively expressed in transcriptionally active or inactive domains thus acting as a biomarker of overall DNA repair [ 28 , 29 ].…”

Section: Introductionmentioning

confidence: 99%

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REC‐2006—A Fractionated Extract of Podophyllum hexandrum Protects Cellular DNA from Radiation‐Induced Damage by Reducing the Initial Damage and Enhancing Its Repair In Vivo

Chaudhary

Shukla

Sharma

2011

Evidence-Based Complementary and Alternative Medicine

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Podophyllum hexandrum, a perennial herb commonly known as the Himalayan May Apple, is well known in Indian and Chinese traditional systems of medicine. P. hexandrum has been widely used for the treatment of venereal warts, skin infections, bacterial and viral infections, and different cancers of the brain, lung and bladder. This study aimed at elucidating the effect of REC-2006, a bioactive fractionated extract from the rhizome of P. hexandrum, on the kinetics of induction and repair of radiation-induced DNA damage in murine thymocytes in vivo. We evaluated its effect on non-specific radiation-induced DNA damage by the alkaline halo assay in terms of relative nuclear spreading factor (RNSF) and gene-specific radiation-induced DNA damage via semi-quantitative polymerase chain reaction. Whole body exposure of animals with gamma rays (10 Gy) caused a significant amount of DNA damage in thymocytes (RNSF values 17.7 ± 0.47, 12.96 ± 1.64 and 3.3 ± 0.014) and a reduction in the amplification of β-globin gene to 0, 28 and 43% at 0, 15 and 60 min, respectively. Administrating REC-2006 at a radioprotective concentration (15 mg kg−1 body weight) 1 h before irradiation resulted in time-dependent reduction of DNA damage evident as a decrease in RNSF values 6.156 ± 0.576, 1.647 ± 0.534 and 0.496 ± 0.012, and an increase in β-globin gene amplification 36, 95 and 99%, at 0, 15 and 60 min, respectively. REC-2006 scavenged radiation-induced hydroxyl radicals in a dose-dependent manner stabilized DPPH free radicals and also inhibited superoxide anions. Various polyphenols and flavonoides present in REC-2006 might contribute to scavenging of radiation-induced free radicals, thereby preventing DNA damage and stimulating its repair.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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REC‐2006—A Fractionated Extract of Podophyllum hexandrum Protects Cellular DNA from Radiation‐Induced Damage by Reducing the Initial Damage and Enhancing Its Repair In Vivo

Chaudhary

Shukla

Sharma

2011

Evidence-Based Complementary and Alternative Medicine

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The Influence of the Size of Dose on the Repair Kinetics of X-ray-Induced DNA Strand Breaks Studied in CHO Cells

Dikomey

1993

Medical Radiology

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Unscheduled DNA-synthesis in UV-irradiated spleen lymphocytes of the rat after whole-body X-irradiation

Hollatz

Tempel

1978

Radiat Environ Biophys

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Unscheduled DNA-synthesis of UV-irradiated spleen lymphocytes of the rat is inhibited in a dose-dependent manner by total-body X-irradiation. The reaction is observed immediately after exposure and reaches its maximum within 8--16 h. Doses of some rads are detectable. Following sublethal X-irradiation (250 rad), regeneration takes 4--6 weeks. Analogous results have been obtained in rats treated with 2,4,6-triethyleneimino-1,3,5-triazine. Regardless of the action mechanisms, it is concluded from the present results that the inhibition of the unscheduled incorporation of thymidine-3H in splenic lymphocytes is a very sensitive, though not specific expression of impairment of the lymphatic system.

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Repair of Radiation-induced DNA Strand Breaks in Thymocytes

Cited by 4 publications

References 13 publications

REC‐2006—A Fractionated Extract of Podophyllum hexandrum Protects Cellular DNA from Radiation‐Induced Damage by Reducing the Initial Damage and Enhancing Its Repair In Vivo

REC‐2006—A Fractionated Extract of Podophyllum hexandrum Protects Cellular DNA from Radiation‐Induced Damage by Reducing the Initial Damage and Enhancing Its Repair In Vivo

The Influence of the Size of Dose on the Repair Kinetics of X-ray-Induced DNA Strand Breaks Studied in CHO Cells

Unscheduled DNA-synthesis in UV-irradiated spleen lymphocytes of the rat after whole-body X-irradiation

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