Unscheduled DNA-synthesis in UV-irradiated spleen lymphocytes of the rat after whole-body X-irradiation

Abstract

Unscheduled DNA-synthesis of UV-irradiated spleen lymphocytes of the rat is inhibited in a dose-dependent manner by total-body X-irradiation. The reaction is observed immediately after exposure and reaches its maximum within 8--16 h. Doses of some rads are detectable. Following sublethal X-irradiation (250 rad), regeneration takes 4--6 weeks. Analogous results have been obtained in rats treated with 2,4,6-triethyleneimino-1,3,5-triazine. Regardless of the action mechanisms, it is concluded from the present results that the inhibition of the unscheduled incorporation of thymidine-3H in splenic lymphocytes is a very sensitive, though not specific expression of impairment of the lymphatic system.

No indications of an enhanced UV-light-induced unscheduled DNA synthesis in splenocytes of mice following a low-dose irradiation in vivo or in vitro

“…However, several experiments also yielded a reduced level of UVlight-induced UDS in adapted cells, implying that the differences are rather a result of a variability inherent to this endpoint, than of some induced cellular processes. Similar variabilities of UV-light-induced UDS levels have also been reported by others (Hollatz and Tempel 1978;Cleaver and Thomas 1981).…”

Kinetics of telencephalic neural cell proliferation during the fetal regeneration period following a single X-irradiation at the late organogenesis stage

“…It may be suggested that these events represent an intense DNA-repair replication. However, the magnitude of this process, which can be detected easily by 3H-thymidine labeling, is rather surprising if we compare the Recovery from irradiation injury after 12 h Only partly recovery from irradiation injury after 8-12 h underlying replication intensity with other reports on the same subject (Yoshizawa et al 1978;Tempel 1979;Hollatz and Tempel 1978). These observations lead to the question of the underlying principles leading to this topological reparation diversity.…”

Topoisomerase II. Its role in conformation changes in superhelical nuclear DNA of lymphocytes after γ-irradiation

“…Он составляет 62 % после 10 мин инкубации и достигает 83 % спустя 1 ч после ее начала. Следовательно, наши ре-зультаты, как и данные литературы [11,12], свидетельствуют о том, что в лимфоцитах, находящихся в стадии Go, после воздействия ионизирующей радиации осуществляется репаративный синтез ДНК, который как этап эксцизионной репарации, по-видимому, соответствует (в свете наших вышеизложенных представлений) фазе первоначального восстановления суперспиральной структуры нуклеоида в этих клетках.…”