Repair of Large-Scale Rib Defects Based on Steel-Reinforced Concrete-Designed Biomimetic 3D-Printed Scaffolds with Bone-Mineralized Microenvironments

Bai, Baoshuai; Hao, Junxiang; Hou, Mengjie; Wang, Tao; Wu, Xiaodi; Liu, Yanhan; Wang, Yiyang; Dai, Chengxiang; Hua, Yujie; Ji, Guangyue; Zhou, Guangdong

doi:10.1021/acsami.2c08422

Cited by 5 publications

(7 citation statements)

References 64 publications

(91 reference statements)

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“…The centrifuge tube was incubated overnight on a shaker at 37 °C. The pellet was collected in a dialysis bag and placed in a tank containing a sufficient amount of deionized water for two days to remove the residual EDC and NHS; the resulting pellets were dried again using a freeze-drying machine to produce the final HA-GT microcarrier scaffold …”

Section: Methodsmentioning

confidence: 99%

Cartilage Regeneration Units Based on Hydrogel Microcarriers for Injectable Cartilage Regeneration in an Autologous Goat Model

Song

et al. 2023

ACS Biomater. Sci. Eng.

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Despite numerous studies on tissue-engineered injectable cartilage, it is still difficult to realize stable cartilage formation in preclinical large animal models because of suboptimal biocompatibility, which hinders further application in clinical settings. In this study, we proposed a novel concept of cartilage regeneration units (CRUs) based on hydrogel microcarriers for injectable cartilage regeneration in goats. To achieve this goal, hyaluronic acid (HA) was chosen as the microparticle to integrate gelatin (GT) chemical modification and a freeze-drying technology to create biocompatible and biodegradable HA-GT microcarriers with suitable mechanical strength, uniform particle size, a high swelling ratio, and cell adhesive ability. CRUs were then prepared by seeding goat autologous chondrocytes on the HA-GT microcarriers and culturing in vitro. Compared with traditional injectable cartilage methods, the proposed method forms relatively mature cartilage microtissue in vitro and improves the utilization rate of the culture space to facilitate nutrient exchange, which is necessary for mature and stable cartilage regeneration. Finally, these precultured CRUs were used to successfully regenerate mature cartilage in nude mice and in the nasal dorsum of autologous goats for cartilage filling. This study provides support for the future clinical application of injectable cartilage.

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Section: Methodsmentioning

confidence: 99%

Cartilage Regeneration Units Based on Hydrogel Microcarriers for Injectable Cartilage Regeneration in an Autologous Goat Model

Song

et al. 2023

ACS Biomater. Sci. Eng.

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Section: Bone Regenerative Fixationmentioning

confidence: 99%

Section: Bone Regenerative Fixationmentioning

confidence: 99%

“…BRU-DBM scaffolds demonstrated great therapeutic efficacy on treating large bone defects (Figure c). Similarly, we also constructed a 3D printed bone biomimetic scaffold (BBS) (sPG) which was then integrated with BMSCs (BMSCs-loaded sPG) . BMSCs-loaded sPG was implanted in the rib defects of rabbits and demonstrated satisfactory bone regeneration effects (Figure d).…”

Section: Bone Regenerative Fixationmentioning

confidence: 99%

“…Similarly, we also constructed a 3D printed bone biomimetic scaffold (BBS) (sPG) which was then integrated with BMSCs (BMSCs-loaded sPG). 102 BMSCsloaded sPG was implanted in the rib defects of rabbits and demonstrated satisfactory bone regeneration effects (Figure 5d). In addition, we also studied the effect of the regulated pathology microenvironment on bone regeneration via a building bone regeneration multicellular unit (BRMU).…”

Section: Bone Regenerative Fixationmentioning

confidence: 99%

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Biodegradable Implants for Internal Fixation of Fractures and Accelerated Bone Regeneration

et al. 2023

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Bone fractures have always been a burden to patients due to their common occurrence and severe complications. Traditionally, operative treatments have been widely used in the clinic for implanting, despite the fact that they can only achieve bone fixation with limited stability and pose no effect on promoting tissue growth. In addition, the nondegradable implants usually need a secondary surgery for implant removal, otherwise they may block the regeneration of bones resulting in bone nonunion. To overcome the low degradability of implants and avoid multiple surgeries, tissue engineers have investigated various biodegradable materials for bone regeneration, whereas the significance of stability of long-term bone fixation tends to be neglected during this process. Combining the traditional orthopedic implantation surgeries and emerging tissue engineering, we believe that both bone fixation and bone regeneration are indispensable factors for a successful bone repair. Herein, we define such a novel idea as bone regenerative fixation (BRF), which should be the main future development trend of biodegradable materials.

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Tolerant and Rapid Endochondral Bone Regeneration Using Framework‐Enhanced 3D Biomineralized Matrix Hydrogels

Bai,

Liu,

Huang

et al. 2023

Advanced Science

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Tissue‐engineered bone has emerged as a promising alternative for bone defect repair due to the advantages of regenerative bone healing and physiological functional reconstruction. However, there is very limited breakthrough in achieving favorable bone regeneration due to the harsh osteogenic microenvironment after bone injury, especially the avascular and hypoxic conditions. Inspired by the bone developmental mode of endochondral ossification, a novel strategy is proposed for tolerant and rapid endochondral bone regeneration using framework‐enhanced 3D biomineralized matrix hydrogels. First, it is meticulously designed 3D biomimetic hydrogels with both hypoxic and osteoinductive microenvironment, and then integrated 3D‐printed polycaprolactone framework to improve their mechanical strength and structural fidelity. The inherent hypoxic 3D matrix microenvironment effectively activates bone marrow mesenchymal stem cells self‐regulation for early‐stage chondrogenesis via TGFβ/Smad signaling pathway due to the obstacle of aerobic respiration. Meanwhile, the strong biomineralized microenvironment, created by a hybrid formulation of native‐constitute osteogenic inorganic salts, can synergistically regulate both bone mineralization and osteoclastic differentiation, and thus accelerate the late‐stage bone maturation. Furthermore, both in vivo ectopic osteogenesis and in situ skull defect repair successfully verified the high efficiency and mechanical maintenance of endochondral bone regeneration mode, which offers a promising treatment for craniofacial bone defect repair.

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Repair of Large-Scale Rib Defects Based on Steel-Reinforced Concrete-Designed Biomimetic 3D-Printed Scaffolds with Bone-Mineralized Microenvironments

Cited by 5 publications

References 64 publications

Cartilage Regeneration Units Based on Hydrogel Microcarriers for Injectable Cartilage Regeneration in an Autologous Goat Model

Cartilage Regeneration Units Based on Hydrogel Microcarriers for Injectable Cartilage Regeneration in an Autologous Goat Model

Biodegradable Implants for Internal Fixation of Fractures and Accelerated Bone Regeneration

Tolerant and Rapid Endochondral Bone Regeneration Using Framework‐Enhanced 3D Biomineralized Matrix Hydrogels

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