Repair of Cartilage Defects in Arthritic Tissue with Differentiated Human Embryonic Stem Cells

Olee, Tsaiwei; Grogan, Shawn P.; Lotz, Martin; Colwell, Clifford W.; D’Lima, Darryl D.; Snyder, Evan Y.

doi:10.1089/ten.tea.2012.0751

Cited by 21 publications

(22 citation statements)

References 57 publications

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“…Sections were stained with Alcian Blue, Hematoxylin and Eosin (H&E), Safranin O/Methyl green, and Toluidine Blue as indicated [20,29,32]. For immunohistochemistry, the primary antibody information could be found in Supplementary Table S1.…”

Section: Histology and Immunohistochemistrymentioning

confidence: 99%

Chemically defined serum-free conditions for cartilage regeneration from human embryonic stem cells

et al. 2016

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Section: Histology and Immunohistochemistrymentioning

confidence: 99%

Chemically defined serum-free conditions for cartilage regeneration from human embryonic stem cells

et al. 2016

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“…Chondrogenic properties of these cells have been confirmed by recent studies [36,[79][80][81]. Utilization of iPSC can afford abundant availability of cell source as well as allogenic transplantation, which reduces invasion and cost.…”

Section: Other Cell Sourcesmentioning

confidence: 83%

Cartilage repair techniques in the knee: stem cell therapies

Yoshiya

Dhawan

2015

Curr Rev Musculoskelet Med

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Among the surgical options for large full-thickness chondral injuries, cell-based therapy has been practiced and its satisfactory outcomes have been reported. One area that appears promising is cell-based therapies utilizing stem cells. Various tissues within the human body contain mesenchymal stem cells (MSCs) from where these can be harvested. These include bone marrow, adipose, synovium, peripheral blood, and umbilical cord. In this article, both preclinical animal studies and clinical studies dealing with the use of MSCs for cartilage repair of the knee are reviewed. Majority of the clinical papers have shown promising results; however, there are a limited number of studies of high evidence level. Clinical significance of the stem cell therapy as compared to other surgical options as well as optimization of the procedure in terms of cell type and delivery method is still to be determined.

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“…Implanted cells could directly replace the physical and biological functions of chondrocytes within articular cartilage and indirectly guide the recipient's neighboring cells to support integration by the secretion of trophic signals. Through the use of stem cells, tissue engineering may be enable derivation of functional chondrocytes that express mature markers of differentiation and are functional in a threedimensional architecture, recapitulating the nuances of the invivo counterparts physiologically and anatomically [12,13].…”

Section: Articular Cartilage Disease Trauma and Conventional Therapymentioning

confidence: 99%

Stem Cell-Based Repair and Regeneration of Articular Cartilage

Paek¹,

Kim²,

Tuan³

2017

JSRT

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Articular cartilage is a highly specialized tissue, that when critically injured has an extremely limited capacity for regeneration. Accordingly, a clinically acceptable treatment option without risks and recurrence is currently unavailable. Inadequately treated destructive and degenerative cartilage injuries will often develop into progressive joint degeneration or osteoarthritis. Conventional surgical treatments frequently produce fibrocartilage, which cannot support the original cartilage function and deteriorates rapidly, while other conservative therapies only offer symptomatic relief. Here, we review the current tissue engineering technology for cartilage repair and describe our efforts to develop advanced cell-based engineered constructs to replace structural and biological functions, and to facilitate the regeneration of new cartilage. To overcome the limited source of available autologous chondrocytes provide only a limited population for growth and repair, hence the utility of adult bone marrow derived mesenchymal stem cells (MSCs) have been actively investigated. Biocompatible and biodegradable scaffolds, including poly-ε-caprolactone, poly-L-lactic acid, alginate, and collagen type I, have also been evaluated for their physical maneuverability, compatibility, and structural support of mesenchymal stem cells integrated into host cartilage tissue. The combination of MSCs with biomaterial scaffolds produced hyaline cartilage-like tissue with smooth articular surfaces, biochemical compositions most like that of native cartilage, and with stronger mechanical properties. As bone marrow derived MSCs are typically extracted by rather invasive means, recent studies suggest that adipose-derived stromal cells may provide similar therapeutic benefits with isolation methods that are less invasive. Based on a growing body of evidence, future strategies should clarify the role of MSCs and perhaps consider the use of adipose-derived MSCs combined with a durable and physiologically compatible biological scaffold.

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Repair of Cartilage Defects in Arthritic Tissue with Differentiated Human Embryonic Stem Cells

Cited by 21 publications

References 57 publications

Chemically defined serum-free conditions for cartilage regeneration from human embryonic stem cells

Chemically defined serum-free conditions for cartilage regeneration from human embryonic stem cells

Cartilage repair techniques in the knee: stem cell therapies

Stem Cell-Based Repair and Regeneration of Articular Cartilage

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