Repair of cartilage defect in the rabbit with cultured mesenchymal stem cells from bone marrow

Abstract

In 16 mature New Zealand white rabbits mesenchymal stem cells were aspirated from the bone marrow, cultured in monolayer and implanted on to a full-thickness osteochondral defect artificially made on the patellar groove of the same rabbit. A further 13 rabbits served as a control group. The rabbits were killed after 14 weeks. Healing of the defect was investigated histologically using haematoxylin and eosin and Safranin-O staining and with immunohistochemical staining for type-II collagen. We also used a reverse transcription-polymerase chain reaction (RT-PCR) to detect mRNA of type-I and type-II collagen. The semiquantitative histological scores were significantly higher in the experimental group than in the control group (p < 0.05). In the experimental group immunohistochemical staining on newly formed cartilage was more intense for type-II collagen in the matrix and RT-PCR from regenerated cartilage detected mRNA for type-II collagen in mature chondrocytes. These findings suggest that repair of cartilage defects can be enhanced by the implantation of cultured mesenchymal stem cells.

Chondrogenesis of Human Adipose-Derived Stem Cells by In Vivo Co-graft with Auricular Chondrocytes from Microtia

“…Previous studies found that grafting of BMSCs could repair defects of the articular cartilage of rabbits by differentiating into chondrocytes and forming cartilaginous tissues [20,21]. Zhang's group successfully regenerated humanear-shaped cartilage by co-grafting human microtia chondrocytes with goat BMSCs in nude mice [4].…”

“…These results indicate that co-culture with chondrocytes could properly promote the chondrogenic differentiation of BMSCs, which may serve as a promising strategy for cartilage reconstruction. However, due to the limited sources of human BMSCs, animal BMSCs were dominantly employed for studies of chondrogenesis [4,20,21]. Albeit the drawing of bone marrow brings extra pain for patients, the application of BMSCs for regenerative medicine is not widely restricted.…”

Evaluation of cartilage repair tissue after biomaterial implantation in rat patella by using T2 mapping

“…in rabbit [13], goat [14], dog [15] and rat [16,17]. Recent works have also assessed the efficacy of different scaffolds containing embedded chondrocytes or multipotent stem cells to fill osteochondral defects [18][19][20]. In tissue engineering, several factors must be taken into account: (1) localization, depth and diameter of the chondral lesion, (2) the animal species considered, (3) the kind of scaffold and cells implanted, and (4) the programming of load impact after implantation.…”

Bedeutung von Östrogenen für Knorpelgewebe und Bandscheiben

“…Über verschiedene Evidenzwege lässt sich zeigen, das Östradiol mit der Synthese und Sekretion von Wachstumsfaktoren interagieren kann, darunter vor allem mit TGF("transforming growth factor")-β und IGF("insulin-like growth factor")-1. TGF-β reguliert die Chondrozytenproliferation und -differenzierung und kann sogar die Differenzierung von mesenchymalen Stammzellen in Chondrozyten stimulieren [24]. Die TGF-β-Expression wird dosisabhängig und biphasisch von Östradiol beeinflusst: Die Expression erhöht sich bei niedrigen (0,1 nmol/l) und verringert sich bei supraphysiologischen (10 nmol/l) Östradiol-konzentrationen [34].…”