Chondrogenesis of Human Adipose-Derived Stem Cells by In Vivo Co-graft with Auricular Chondrocytes from Microtia

Cai, Zhen; Pan, Bo; Jiang, Haiyue; Zhang, Lixia

doi:10.1007/s00266-015-0481-0

Cited by 22 publications

(25 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Co-culture and co-implantation of MSCs with AuCs has also been demonstrated with animal cells [ 23 , 25 ] and human-animal cell hybrids [ 21 , 22 ]. Only one previous co-implantation study combined both AuCs and stem cells of human origin, co-implanting AuCs with adipose-derived stem cells [ 24 ]. Similar to previous co-implantation studies, we observed comparable auricular cartilage generation between monocellular AuC and combined cellular implanted discs, while we also demonstrated that co-implantation of human cells can be extended to a full-scale ear construct.…”

Section: Discussionmentioning

confidence: 99%

“…However, little is known about the behavior of AuCs in combination with MSCs. The co-implantation of AuCs with MSCs [ 21 – 23 ] or adipose-derived stem cells [ 24 ] has generated cartilage in vivo , yet the impact of these studies is limited due to the use of non-human cells [ 21 – 23 , 25 ], a lack of markers specific to elastic cartilage [ 21 , 22 ], or the absence of mechanical evaluation [ 22 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Tissue engineering the human auricle by auricular chondrocyte-mesenchymal stem cell co-implantation

et al. 2018

View full text Add to dashboard Cite

Children suffering from microtia have few options for auricular reconstruction. Tissue engineering approaches attempt to replicate the complex anatomy and structure of the ear with autologous cartilage but have been limited by access to clinically accessible cell sources. Here we present a full-scale, patient-based human ear generated by implantation of human auricular chondrocytes and human mesenchymal stem cells in a 1:1 ratio. Additional disc construct surrogates were generated with 1:0, 1:1, and 0:1 combinations of auricular chondrocytes and mesenchymal stem cells. After 3 months in vivo, monocellular auricular chondrocyte discs and 1:1 disc and ear constructs displayed bundled collagen fibers in a perichondrial layer, rich proteoglycan deposition, and elastin fiber network formation similar to native human auricular cartilage, with the protein composition and mechanical stiffness of native tissue. Full ear constructs with a 1:1 cell combination maintained gross ear structure and developed a cartilaginous appearance following implantation. These studies demonstrate the successful engineering of a patient-specific human auricle using exclusively human cell sources without extensive in vitro tissue culture prior to implantation, a critical step towards the clinical application of tissue engineering for auricular reconstruction.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Tissue engineering the human auricle by auricular chondrocyte-mesenchymal stem cell co-implantation

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Mesenchymal stem cells (MSCs) are a promising alternative cell source for cartilage repair. When appropriately stimulated, MSCs can differentiate into a variety of cell types, including cartilage-producing chondrocytes [ 4 , 15 – 19 ]. MSCs are frequently isolated from bone marrow.…”

Section: Introductionmentioning

confidence: 99%

“…In any case, common strategies to differentiate adult stem cells into chondrocytes still require the use of costly growth factors in the culture medium, such as transforming growth factor betas (TGF- β s), insulin-like growth factors, and bone morphogenetic proteins (BMPs). Of note, these additives could potentially lead to unexpected side effects during use in clinical practice [ 15 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

Calcium/Cobalt Alginate Beads as Functional Scaffolds for Cartilage Tissue Engineering

Focaroli

Teti

Salvatore

et al. 2016

Stem Cells International

View full text Add to dashboard Cite

Articular cartilage is a highly organized tissue with complex biomechanical properties. However, injuries to the cartilage usually lead to numerous health concerns and often culminate in disabling symptoms, due to the poor intrinsic capacity of this tissue for self-healing. Although various approaches are proposed for the regeneration of cartilage, its repair still represents an enormous challenge for orthopedic surgeons. The field of tissue engineering currently offers some of the most promising strategies for cartilage restoration, in which assorted biomaterials and cell-based therapies are combined to develop new therapeutic regimens for tissue replacement. The current study describes the in vitro behavior of human adipose-derived mesenchymal stem cells (hADSCs) encapsulated within calcium/cobalt (Ca/Co) alginate beads. These novel chondrogenesis-promoting scaffolds take advantage of the synergy between the alginate matrix and Co+2 ions, without employing costly growth factors (e.g., transforming growth factor betas (TGF-βs) or bone morphogenetic proteins (BMPs)) to direct hADSC differentiation into cartilage-producing chondrocytes.

show abstract

“…The level expression of Elastin was suggested that the production of elastin protein requires a longer period of cultures. Previous studies also reported that the bone marrow stem required 28 day of induction to express the high expression gene of Elastin (Cai et al 2015;Zhang et al 2015).…”

Section: Effect Of Tgf-βs On the Expression Of Chondrogenic Gene In Hmentioning

confidence: 90%

Transforming Growth Factor Beta 3 Induced Human Adipose-derived Stem Cells for Auricular Chondrogenesis

Omar

Goh²,

Ubaidah

et al. 2018

JSM

View full text Add to dashboard Cite

The limitation of self-repair and proliferation capacity of chondrocytes in cartilage reconstruction lead to alternative search of cell source that can improve the auricular regeneration. Human adipose-derived stem cells (HADSC) are an alternative cell source that have unique characteristics to self-renew and differentiate into various tissues making it suitable for cell therapy and tissue engineering. This study aimed to examine the chondrogenic differentiation potential of (HADSC) in monolayer culture by the presence of different transforming growth factor beta's, TFG-β1,-β2 and-β3. HADSC at passage 3 (1.5 × 10 5 cell/mL) were cultured in chondrogenic medium containing 5 ng/mL of different transforming growth factor beta's, TFG-β1,-β2 and-β3 for 7, 14 and 21 days. Data analysis was evaluated based on the growth rate of cells, cells morphological changed, production of collagen type II and glycosaminoglycan sulphate (sGAG). The quantitative RT-PCR was carried out to determine the chondrogenic, fibrogenic and hypertrophic gene expression levels. Differentiation of HADSC into chondrocytes using TFG-β indicates the occurrence of the chondrogenesis process. The best chondrogenic differentiation was observed in HADSC induced by TFG-β3 through the chondrocytes-like cells morphology with cells aggregation and high production of proteoglycan matrices compared to other TGF-βs groups. Additionally, the expression of chondrocytes-specific genes such as Type II collagen, Aggrecan core protein, Elastin and Sox 9 was high. In conclusion, this study has showed that TGF-β3 is the potential growth factor in producing chondrogenic cells for auricular cartilage tissue engineering.

show abstract

Chondrogenesis of Human Adipose-Derived Stem Cells by In Vivo Co-graft with Auricular Chondrocytes from Microtia

Cited by 22 publications

References 21 publications

Tissue engineering the human auricle by auricular chondrocyte-mesenchymal stem cell co-implantation

Tissue engineering the human auricle by auricular chondrocyte-mesenchymal stem cell co-implantation

Calcium/Cobalt Alginate Beads as Functional Scaffolds for Cartilage Tissue Engineering

Transforming Growth Factor Beta 3 Induced Human Adipose-derived Stem Cells for Auricular Chondrogenesis

Contact Info

Product

Resources

About