Repair of calvarial defects in rabbits with platelet-rich plasma as the scaffold for carrying bone marrow stromal cells

Jiang, Zhi-qing; Liu, Huanye; Zhang, Liping; Wu, Zhiqiang; Shang, Dezhi

doi:10.1016/j.tripleo.2011.03.026

Cited by 30 publications

(24 citation statements)

References 31 publications

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“…The characteristic morphology and the molecular level by flow cytometry of MSC cultivated in this study was the same described by other studies for identifying murine MSC (Badillo et al 2007, Falanga et al 2007, Inoue et al 2008, Kwon et al 2008, Mehta et al 2010, Jiang et al 2011, Kim et al 2011.…”

Section: Discussionmentioning

confidence: 76%

“…Previous research has shown that hyperglycemia induces a state of mild chronic inflammation, which interferes with the balance of the production of cytokines involved in tissue repair (Hughson et al 2011, Jiang et al 2011. The more intense cicatrization observed in the lesions of this group can be attributed to the immunomodulation characteristics and plasticity of the MSC.…”

Section: Discussionmentioning

confidence: 89%

“…The higher percentage of collagen in the lesions of mice from the group GSC indicates intense fibroblastic action, seen that these are one the most responsible cells for the replacement of are the fibronectin and proteoglycan of the extra cellular matrix per collagen, main component of the scar in formation (Balbino et al 2005). Besides, according to contemporary studies (Jiang et al 2011, Kim et al 2011, indicate that the cutaneous lesions of the mice in group GSC are in an advanced cicatrization stage, characterized by the formation of stronger and elastic connective tissue.…”

Section: Discussionmentioning

confidence: 93%

“…Because DM changes the expression of cytokines (Buschard 2011, Hughson et al 2011, Jiang et al 2011, Touvra et al 2011, there remain large uncertainties of cellular components of the diabetic patient will respond successfully to stimulate the skin heals.…”

Section: Discussionmentioning

confidence: 99%

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Role of the autologous mesenchymal stem cells compared with platelet rich plasma on cicatrization of cutaneous wounds in diabetic mice

et al. 2016

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RESUMO.-[Papel das células-tronco mesenquimais autólogas em comparação com plasma rico em plaquetas na cicatrização de feridas cutâneas em camundongos diabéticos.] Lesões cutâneas crônicas afetam 15% dos pacientes diabéticos e humanos representam um risco 15 a 46 vezes maior de amputações de membros em comparação com as pessoas com a glicemia normal. Supõe-se que a metade destas amputações poderia ser evitada por meio do tratamento precoce das feridas cutâneas com, por exemplo, uma adequada terapia celular. Objetivos: Neste estudo, a ação do transplante autólogo de células estaminais mesenquimais (MSC) foi avaliada em comparação com o tratamento com plasma rico em plaquetas autólogo (PRP) na cicatrização de lesões cutâneas induzidas em camundongos diabéticos. Estes animais foram previamente tratados com estreptozotocina para induzir diabetes mellitus e feridas redondas de 1,5 cm de diâmetro foram criadas na região posterior. Os diâmetros dos ferimentos e tempo de cicatrização foram avaliados durante 30 dias e os resultados fo- (7):617-624. Departamento de Clínica e Cirurgia Veterinária, Universidade Federal do Piauí, Campus Socopo, Teresina, PI 64049-550, Brazil. E-mail: argolo_napoleao@ufpi.edu.br Chronic cutaneous lesions affect 15% of diabetic human patients and represent a risk 15 to 46 times larger of limb amputations compared to people with normal glycemia. It is assumed that half of these amputations could be prevented by early treatment of wounds, for example, with proper cell therapy. Objectives: In this study, the action of the autologous transplant of mesenchymal stem-cells (MSC) was evaluated compared to the treatment with autologous platelet rich plasma (PRP) in the cicatrization of cutaneous lesions induced in diabetic mice. These animals were previously treated with streptozootocin to induce diabetes mellitus and round wounds of 1.5cm in diameter were created in the posterior region. Diameters of the wounds and healing time were evaluated during 30 days and the results were submitted to variance analysis and Tukey's test average. It was noticed that the animals treated with MSC presented a more accelerated cicatrization of the cutaneous lesion than the animals treated with PRP. However, the treatment with PRP presented better results than just the daily asepsis of the lesions with saline or covering them with semi-permeable bandage. Besides, the use of semi-permeable bandage kept the cutaneous lesions of diabetic mice did not interfere negatively with cicatrization, proved to be harmless to use, but kept the cutaneous lesions more hydrated than the ones exposed to the environment.

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Section: Discussionmentioning

confidence: 76%

Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

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Role of the autologous mesenchymal stem cells compared with platelet rich plasma on cicatrization of cutaneous wounds in diabetic mice

et al. 2016

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“…The calvaria and tibia are the most used areas for bone regeneration studies. (10,11) Barrier membranes are tested in a lot of experimental studies of bone regeneration and they are widely used in clinical therapy. (12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22) The placement of a barrier membrane promotes bone formation because it does not allow non-osteogenic soft tissue to infiltrate in the bone defect.…”

Section: Results and Discussion Results And Discussionmentioning

confidence: 99%

Use of Collagen, PTFE and PRF Membranes in Bone Reconstruction an Experimental and Histomorphometric Study

Neculae¹,

Anghelescu²,

Zurac³

et al. 2017

JTMR

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Bone regeneration techniques cannot be done without barrier membranes, even if horizontal or vertical ridge augmentation and socket ridge preservation is taken into consideration. This study presents a comparison between outcomes of bone regeneration, after producing standardized bone defects followed by covering them with membranes, on an animal experimental model. The study was conducted on 18 New Zeeland rabbits, by creating 2 defects in the left tibial bone of each rabbit: one standardized defect with a diameter of 4 mm, and the second by creating 5 monocortical holes with a small round bur. The defects were augmented with bovine bone, beta-tricalcium phosphate and perioglass and they were covered with 3 types of membrane: collagen (12 defects -group A), PTFE membrane (12 defects -group B) and PRF membrane, made from the blood of the same rabbit (12 defects -group C). The animals were sacrificed after 6 months and analysed histomorphometrically. The new bone around graft particles has a thickness of 98.26 μm for collagen membrane, 49.19 μm for PTFE membrane and 63.98 μm for PRF membrane. The density of osteoblasts and osteocytes has an average of 0.0012 for collagen membrane, 0.0009 for PTFE membrane and 0.0010 for PRF membrane. Regarding the collagen membrane, it is observed that when used the bone regeneration appears to have a higher density of osteoforming cells and a higher quantity of new bone

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Serum albumin enhances bone healing in a nonunion femoral defect model in rats: a computer tomography micromorphometry study

Skaliczki

Schandl

Weszl

et al. 2013

International Orthopaedics (SICOT)

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Repair of calvarial defects in rabbits with platelet-rich plasma as the scaffold for carrying bone marrow stromal cells

Cited by 30 publications

References 31 publications

Role of the autologous mesenchymal stem cells compared with platelet rich plasma on cicatrization of cutaneous wounds in diabetic mice

Role of the autologous mesenchymal stem cells compared with platelet rich plasma on cicatrization of cutaneous wounds in diabetic mice

Use of Collagen, PTFE and PRF Membranes in Bone Reconstruction an Experimental and Histomorphometric Study

Serum albumin enhances bone healing in a nonunion femoral defect model in rats: a computer tomography micromorphometry study

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