Repair of articular cartilage defects by tissue-engineered cartilage constructed with adipose-derived stem cells and acellular cartilaginous matrix in rabbits

Wang, Z J; An, R Z; Zhao, Jin; Zhang, Q; Yang, Jun; Wang, J B; Wen, Gen; Yuan, Xingliang; Qi, Xin; Li, S J; Ye, X C

doi:10.4238/2014.june.18.2

Cited by 31 publications

(31 citation statements)

References 23 publications

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“…4), which was corroborated by previous studies [17, 19]. Most importantly, decellularization did not alter the chondroinductive properties of the CDM, which has been extensively documented in previous studies that demonstrated CDM retained its chondroinductive capacity after decellularization [1, 8, 10, 12, 15–17, 19, 21, 22, 25–27, 30, 32, 33, 71]. After chondrogenic culture, CDM constructs adopted a smooth shiny texture indicative of new matrix synthesis (Fig.…”

Section: Discussionsupporting

confidence: 85%

“…Of particular note is the finding that, depending on the cell type, CDM can promote chondrogenic differentiation in the absence of exogenous growth factors [1, 6, 9, 12, 20, 24, 25], or exhibit a synergistic effect with growth factor supplementation [1, 7, 11–13, 18, 27, 28]. CDM has also been shown to enhance the in vivo repair of cartilage defects [8, 10, 16, 19, 26]. Furthermore, studies comparing collagen-hyaluronic acid scaffolds to CDM constructs illustrated that CDM constructs retained newly synthesized glycosaminoglycans better than collagen-hyaluronic acid scaffolds [13].…”

Section: Introductionmentioning

confidence: 99%

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Fabrication of anatomically-shaped cartilage constructs using decellularized cartilage-derived matrix scaffolds

2016

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The native extracellular matrix of cartilage contains entrapped growth factors as well as tissue-specific epitopes for cell-matrix interactions, which make it a potentially attractive biomaterial for cartilage tissue engineering. A limitation to this approach is that the native cartilage extracellular matrix possesses a pore size of only a few nanometers, which inhibits cellular infiltration. Efforts to increase the pore size of cartilage-derived matrix (CDM) scaffolds dramatically attenuate their mechanical properties, which makes them susceptible to cell-mediated contraction. In previous studies, we have demonstrated that collagen crosslinking techniques are capable of preventing cell-mediated contraction in CDM disks. In the current study, we investigated the effects of CDM concentration and pore architecture on the ability of CDM scaffolds to resist cell-mediated contraction. Increasing CDM concentration significantly increased scaffold mechanical properties, which played an important role in preventing contraction, and only the highest CDM concentration (11% w/w) was able to retain the original scaffold dimensions. However, the increase in CDM concentration led to a concomitant decrease in porosity and pore size. Generating a temperature gradient during the freezing process resulted in unidirectional freezing, which aligned the formation of ice crystals during the freezing process and in turn produced aligned pores in CDM scaffolds. These aligned pores increased the pore size of CDM scaffolds at all CDM concentrations, and greatly facilitated infiltration by mesenchymal stem cells (MSCs). These methods were used to fabricate of anatomically-relevant CDM hemispheres. CDM hemispheres with aligned pores supported uniform MSC infiltration and matrix deposition. Furthermore, these CDM hemispheres retained their original architecture and did not contract, warp, curl, or splay throughout the entire 28-day culture period. These findings demonstrate that given the appropriate fabrication parameters, CDM scaffolds are capable of maintaining complex structures that support MSC chondrogenesis.

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Section: Discussionsupporting

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Fabrication of anatomically-shaped cartilage constructs using decellularized cartilage-derived matrix scaffolds

2016

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“…One study used cells of P6 [54], and another study used cells between P4 and P7 [48]. No relationship was apparent between chondrogenesis and number of passages.…”

Section: Resultsmentioning

confidence: 99%

“…Thirteen studies (11%) used adipose stem cells [54, 58–69], six (5%) used synovia [70–75] and three (2%) used periostium-derived MSCs [76–78]. Three studies (3%) used embryonic stem cell-derived MSCs [79–81] whereas 2 studies (2%) used muscle-derived MSCs [82, 83].…”

Section: Resultsmentioning

confidence: 99%

The use of mesenchymal stem cells for cartilage repair and regeneration: a systematic review

et al. 2017

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BackgroundThe management of articular cartilage defects presents many clinical challenges due to its avascular, aneural and alymphatic nature. Bone marrow stimulation techniques, such as microfracture, are the most frequently used method in clinical practice however the resulting mixed fibrocartilage tissue which is inferior to native hyaline cartilage. Other methods have shown promise but are far from perfect. There is an unmet need and growing interest in regenerative medicine and tissue engineering to improve the outcome for patients requiring cartilage repair. Many published reviews on cartilage repair only list human clinical trials, underestimating the wealth of basic sciences and animal studies that are precursors to future research. We therefore set out to perform a systematic review of the literature to assess the translation of stem cell therapy to explore what research had been carried out at each of the stages of translation from bench-top (in vitro), animal (pre-clinical) and human studies (clinical) and assemble an evidence-based cascade for the responsible introduction of stem cell therapy for cartilage defects.Main body of abstractThis review was conducted in accordance to PRISMA guidelines using CINHAL, MEDLINE, EMBASE, Scopus and Web of Knowledge databases from 1st January 1900 to 30th June 2015. In total, there were 2880 studies identified of which 252 studies were included for analysis (100 articles for in vitro studies, 111 studies for animal studies; and 31 studies for human studies). There was a huge variance in cell source in pre-clinical studies both of terms of animal used, location of harvest (fat, marrow, blood or synovium) and allogeneicity. The use of scaffolds, growth factors, number of cell passages and number of cells used was hugely heterogeneous.Short conclusionsThis review offers a comprehensive assessment of the evidence behind the translation of basic science to the clinical practice of cartilage repair. It has revealed a lack of connectivity between the in vitro, pre-clinical and human data and a patchwork quilt of synergistic evidence. Drivers for progress in this space are largely driven by patient demand, surgeon inquisition and a regulatory framework that is learning at the same pace as new developments take place.

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“…For example, Hedayatpour et al (2013) stated that ATSCs promoted renewal of damaged myelin sheaths around nerve fibres in mice with artificially induced sclerosis multiplex. Also Wang et al (2014) described improved regeneration ability of the damaged articular cartilages after therapy with ATSCs in rabbits. On the contrary, it was shown that ATSCs have potential to interact with cancer cells (Kucerova et al 2011).…”

Section: Introductionmentioning

confidence: 98%

Ultra-structural morphology of long-term cultivated white adipose tissue-derived stem cells

et al. 2015

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White adipose tissue was long perceived as a passive lipid storage depot but it is now considered as an active and important endocrine organ. It also harbours not only adipocytes and vascular cells but also a wide array of immunologically active cells, including macrophages and lymphocytes, which may induce obesity-related inflammation. Recently, adipose tissue has been reported as a source of adult mesenchymal stem cells with wide use in regenerative medicine and tissue engineering. Their relatively non-complicated procurement and collection (often performed as liposuction during aesthetic surgery) and grand plasticity support this idea even more. We focused our research on exploring the issues of isolation and long-term cultivation of mesenchymal stem cells obtained from adipose tissue. Ultra-structural morphology of the cells cultivated in vitro has been studied and analysed in several cultivation time periods and following serial passages--up to 30 passages. In the first passages they had ultra-structural characteristics of cells with high proteosynthetic activity. Within the cytoplasm, big number of small lipid droplets and between them, sparsely placed, small and inconspicuous, electron-dense, lamellar bodies, which resembled myelin figures were observed. The cells from the later passages contained high number of lamellar electron-dense structures, which filled out almost the entire cytoplasm. In between, mitochondria were often found. These bodies were sometimes small and resembled myelin figures, but several of them reached huge dimensions (more than 1 µm) and their lamellar structure was not distinguishable. We did not have an answer to the question about their function, but they probably represented the evidence of active metabolism of lipids present in the cytoplasm of these cells or represented residual bodies, which arise after the breakdown of cellular organelles, notably mitochondria during long-term cultivation.

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Repair of articular cartilage defects by tissue-engineered cartilage constructed with adipose-derived stem cells and acellular cartilaginous matrix in rabbits

Cited by 31 publications

References 23 publications

Fabrication of anatomically-shaped cartilage constructs using decellularized cartilage-derived matrix scaffolds

Fabrication of anatomically-shaped cartilage constructs using decellularized cartilage-derived matrix scaffolds

The use of mesenchymal stem cells for cartilage repair and regeneration: a systematic review

Ultra-structural morphology of long-term cultivated white adipose tissue-derived stem cells

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