Repair of 8-oxo-7,8-dihydroguanine in prokaryotic and eukaryotic cells: Properties and biological roles of the Fpg and OGG1 DNA N-glycosylases

Boiteux, Serge; Coste, Franck; Castaing, B.

doi:10.1016/j.freeradbiomed.2016.11.042

Cited by 129 publications

(138 citation statements)

References 283 publications

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“…The modified alkaline comet assay allowed us to shed the light on the presence of Fpg‐sensitive DNA sites after captan exposure. As Fpg is a bacterial glycosylase mainly active across oxidized purines (Boiteux et al ), our results corroborate bacterial mutagenicity studies with strains that detect oxidative compounds. Moreover, the sensitivity and the repair defects of CHO cells defective in XRCC1 protein demonstrate the role of BER in overcoming the genotoxic effect of captan.…”

Section: Discussionsupporting

confidence: 86%

Exposure to the Fungicide Captan Induces DNA Base Alterations and Replicative Stress in Mammalian Cells

Fernandez-Vidal

Arnaud

Maumus

et al. 2018

Environ and Mol Mutagen

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The classification of the fungicide captan (CAS Number: 133–06‐2) as a carcinogen agent is presently under discussion. Despite the mutagenic effect detected by the Ames test and carcinogenic properties observed in mice, the genotoxicity of this pesticide in humans is still unclear. New information is needed about its mechanism of action in mammalian cells. Here, we show that Chinese Hamster Ovary (CHO) cells exposed to captan accumulate Fpg‐sensitive DNA base alterations. In CHO and HeLa cells, such DNA lesions require the XRCC1‐dependent pathway to be repaired. Captan also induces a replicative stress that activated the ATR signaling response and resulted in double‐strand breaks and micronuclei. The replicative stress is characterized by a dramatic decrease in DNA synthesis due to a reduced replication fork progression. However, impairment of the XRCC1‐related repair process did not amplify the replicative stress, suggesting that the fork progression defect is independent from the presence of base modifications. These results support the involvement of at least two independent pathways in the genotoxic effect of captan that might play a key role in carcinogenesis. Environ. Mol. Mutagen. 60:286–297, 2019. © 2018 Wiley Periodicals, Inc.

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Section: Discussionsupporting

confidence: 86%

Exposure to the Fungicide Captan Induces DNA Base Alterations and Replicative Stress in Mammalian Cells

Fernandez-Vidal

Arnaud

Maumus

et al. 2018

Environ and Mol Mutagen

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“…Epidemiological studies investigated an association between the Ser326Cys polymorphism and the risk of various types of cancer. An increased risk of cancer was found in some studies, whereas other studies failed to find this association (reviewed in Ali et al 2015; Boiteux, Coste, and Castaing 2017; Goode, Ulrich, and Potter 2002; Hirano 2008; Weiss et al 2005). An increased risk for lung, digestive system, and head and neck cancers was most consistently observed.…”

Section: Introductionmentioning

confidence: 98%

“…A number of sequence variants, referred to as polymorphisms or mutations in the scientific literature, have been reported for OGG1 (reviewed in Goode, Ulrich, and Potter 2002; Weiss et al 2005; Ali et al 2015; Boiteux, Coste, and Castaing 2017). A single nucleotide polymorphism at codon 326 that results in a serine to cysteine amino acid substitution (Ser326Cys) is common in human populations with allelic frequencies of 0.22–0.45 (Goode, Ulrich, and Potter 2002).…”

Section: Introductionmentioning

confidence: 99%

Differential effects of silver nanoparticles on DNA damage and DNA repair gene expression in Ogg1-deficient and wild type mice

et al. 2017

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Due to extensive use in consumer goods, it is important to understand the genotoxicity of silver nanoparticles (AgNPs) and identify susceptible populations. 8-Oxoguanine DNA glycosylase 1 (OGG1) excises 8-oxo-7,8-dihydro-2-deoxyguanine (8-oxoG), a pro-mutagenic lesion induced by oxidative stress. To understand whether defects in OGG1 is a possible genetic factor increasing an individual’s susceptibly to AgNPs, we determined DNA damage, genome rearrangements, and expression of DNA repair genes in Ogg1-deficient and wild type mice exposed orally to 4 mg/kg of citrate-coated AgNPs over a period of 7 d. DNA damage was examined at 3 and 7 d of exposure and 7 and 14 d post-exposure. AgNPs induced 8-oxoG, double strand breaks (DSBs), chromosomal damage, and DNA deletions in both genotypes. However, 8-oxoG was induced earlier in Ogg1-deficient mice and 8-oxoG levels were higher after 7-d treatment and persisted longer after exposure termination. AgNPs downregulated DNA glycosylases Ogg1, Neil1, and Neil2 in wild type mice, but upregulated Myh, Neil1, and Neil2 glycosylases in Ogg1-deficient mice. Neil1 and Neil2 can repair 8-oxoG. Thus, AgNP-mediated downregulation of DNA glycosylases in wild type mice may contribute to genotoxicity, while upregulation thereof in Ogg1-deficient mice could serve as an adaptive response to AgNP-induced DNA damage. However, our data show that Ogg1 is indispensable for the efficient repair of AgNP-induced damage. In summary, citrate-coated AgNPs are genotoxic in both genotypes and Ogg1 deficiency exacerbates the effect. These data suggest that humans with genetic polymorphisms and mutations in OGG1 may have increased susceptibility to AgNP-mediated DNA damage.

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“…Gen ten koduje glikozylazę 8-oksyguaniny, która jest istotnym elementem systemu naprawczego BER [31,34]. Opisany polimorfizm hOGG1 polega na transwersji cytozyny do guaniny, czego skutkiem jest zamiana seryny (Ser) na cysteinę (Cys) w pozycji 326 w łańcuchu polipeptydowym (tab.…”

Section: Pestycydyunclassified

Polymorphism of genes encoding proteins of DNA repair vs. occupational and environmental exposure to lead, arsenic and pesticides

Bukowski¹,

Woźniak²

2017

Med Pr

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StreszczeniePolimorfizm genetyczny wiąże się z występowaniem w populacji co najmniej 2 różnych alleli w danym locus z częstością większą niż 1%. Wyróżniamy m.in. polimorfizm pojedynczego nukleotydu (single nucleotide polymorphism -SNP) i polimorfizm zmiennej liczby powtórzeń tandemowych. Występowanie określonych polimorfizmów w genach kodujących enzymy naprawy DNA jest związane z szybkością i wydajnością naprawy DNA oraz może chronić lub narażać daną osobę na skutki działania określonego ksenobiotyku. Związki chemiczne takie, jak ołów, arsen i pestycydy odznaczają się dużą toksycznością. Opisano wiele różnych polimorfizmów genów kodujących enzymy naprawy DNA, które mają wpływ na skuteczność naprawy uszkodzeń DNA indukowanych przez te ksenobiotyki. W przypadku ołowiu zbadano wpływ polimorfizmów genów: APE1 (apurinic/apyrimidinic endonuclease 1 -endonukleaza miejsca apurynowego/apirymidynowego) (rs1130409), hOGG1 (human 8-oxoguanine glycosylase -glikozylaza 8-oksyguaniny) (rs1052133), XRCC1 (X-ray repair cross-complementing protein group 1 -białko biorące udział w naprawie DNA przez wycinanie zasad) (rs25487), XRCC1 (rs1799782) oraz XRCC3 (X-ray repair cross-complementing protein group 3 -białko biorące udział w naprawie DNA przez rekombinację homologiczną) (rs861539). Dla arsenu przedstawiono w niniejszej pracy wyniki badań dotyczących następujących polimorfizmów: ERCC2 (excision repair cross-complementingbiałko biorące udział w naprawie DNA przez wycinanie nukleotydów) (rs13181), XRCC3 (rs861539), APE1 (rs1130409) oraz hOGG1 (rs1052133). W odniesieniu do pestycydów w pracy przedstawiono zarówno osobny, jak i łączny wpływ polimorfizmów genów takich, jak XRCC1 (rs1799782), hOGG1 (rs1052133), XRCC4 (X-ray repair cross-complementing protein group 4 -białko biorące udział w naprawie DNA przez łączenie końców niehomologicznych) (rs28360135) i genu kodującego enzym detoksykacyjny paraoksonazę PON1 (paraoxonase 1) (rs662). Med. Pr. 2018;69(2):225-235 Słowa kluczowe: polimorfizm genetyczny, arsen, pestycydy, ołów, uszkodzenia DNA, geny naprawy DNA AbstractGenetic polymorphism is associated with the occurrence of at least 2 different alleles in the locus with a frequency higher than 1% in the population. Among polymorphisms we can find single nucleotide polymorphism (SNP) and polymorphism of variable number of tandem repeats. The presence of certain polymorphisms in genes encoding DNA repair enzymes is associated with the speed and efficiency of DNA repair and can protect or expose humans to the effects provoked by xenobiotics. Chemicals, such as lead, arsenic pesticides are considered to exhibit strong toxicity. There are many different polymorphisms in genes encoding DNA repair enzymes, which determine the speed and efficiency of DNA damage repair induced by these xenobiotics. In the case of lead, the influence of various polymorphisms, such as APE1 (apurinic/apyrimidinic endonuclease 1) (rs1130409), hOGG1 (human 8-oxoguanine glycosylase) (rs1052133), XRCC1 (X-ray repair cross-complementing protein group 1) (rs25487), X...

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Repair of 8-oxo-7,8-dihydroguanine in prokaryotic and eukaryotic cells: Properties and biological roles of the Fpg and OGG1 DNA N-glycosylases

Cited by 129 publications

References 283 publications

Exposure to the Fungicide Captan Induces DNA Base Alterations and Replicative Stress in Mammalian Cells

Exposure to the Fungicide Captan Induces DNA Base Alterations and Replicative Stress in Mammalian Cells

Differential effects of silver nanoparticles on DNA damage and DNA repair gene expression in Ogg1-deficient and wild type mice

Polymorphism of genes encoding proteins of DNA repair vs. occupational and environmental exposure to lead, arsenic and pesticides

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