Repair deficiency in Escherichia coli UV-sensitive mutator strain uvr502

Sinzinis, B.I.; Smirnov, G. B.; Saenko, Alexander A.

doi:10.1016/0006-291x(73)91435-6

Cited by 27 publications

(18 citation statements)

References 13 publications

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“…This is not surprising since the mutation in the uvrD gene also confers increased sensitivity to killing by other DNA-damaging agents (Shanabruch et al 1981) and the E. coli uvrD gene product functions in another repair pathway(s) independent of mutH, mutL, and mutS (Siegel 1973;Sinzinis et al 1973).…”

Section: Sensitivity Of S Typhimurium Muth Mutl Muts and Uvrd Mutmentioning

confidence: 95%

The mutH, mutL, mutS, and uvrD Genes of Salmonella typhimurium LT2

Pang¹,

Tsen²,

Lundberg³

et al. 1984

Cold Spring Harbor Symposia on Quantitative Biology

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Section: Sensitivity Of S Typhimurium Muth Mutl Muts and Uvrd Mutmentioning

confidence: 95%

The mutH, mutL, mutS, and uvrD Genes of Salmonella typhimurium LT2

Pang¹,

Tsen²,

Lundberg³

et al. 1984

Cold Spring Harbor Symposia on Quantitative Biology

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“…Although the uvrD3 mutant exhibited extensive UV-stimulated DNA degradation (22), postirradiation hydrolysis of DNA was not abnormally high in the uvrDlOl, recL, or uvrE mutants (26,37,39). uvrE (36,39) and recL (26) mutants, as well as the uvrDlOl mutant used in our study (Fig. 3), were found to be incision proficient.…”

Section: Resultsmentioning

confidence: 59%

Effect of the uvrD mutation on excision repair

Kuemmerle¹,

Masker²

1980

J Bacteriol

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A pair of related Escherichia coli K-12 strains, one of which contains the uvrD101 mutation, were constructed and compared for ability to perform various steps in the excision repair of deoxyribonucleic acid damage inflicted by ultraviolet radiation. The results of this study indicated: (i) ultraviolet sensitivity in the uvrD101 mutant was greater than that of wild type but less than that measured in an incision-deficient uvrA mutant; (ii) host cell reactivation paralleled the survival data; (iii) postirradiation deoxyribonucleic acid degradation was virtually identical in the two strains; (iv) incision, presumably at the sites of pyrimidine dimers, proceeded normally in the uvrD101 strain; (v) excision of pyrimidine dimers was markedly reduced in both rate and extent in the uvrD101 mutant; (vi) the amount of repair resynthesis was the same in both strains, and there was no evidence of abnormally long repair patches in the uvrD mutant; and (vii) rejoining of incision breaks was slow and incomplete in the uvrD strain. These data suggest that the ultraviolet sensitivity conferred by the uvrD mutation arises from inefficient removal of pyrimidine dimers or from failure to close incision breaks. The data are compatible with the notion that the uvrD+ gene produce affects the conformation of incised deoxyribonucleic acid molecules.

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“…Some alleles of uvrD and this mutBI mutant are sensitive to, and made hypermutable by, exposure to alkylating agents (20). Neither uvrD nor mutBI mutants exhibit UV-induced DNA breakdown; however, both mutants are able to produce single-strand incisions in their DNA after UV exposure (23,30). Finally, both mutants appear to have altered recombinational abilities and are mismatch repair deficient (6,16).…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of a UV-sensitive mutator (mutB1) mutant of Haemophilus influenzae

Walter

Stuy

1988

J Bacteriol

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The mutBI mutant of Haemophilus influenzae is very sensitive to UV radiation but only slightly sensitive to methylmethane sulfonate or N-methyl-N'-nitro-N-nitrosoguanidine. Cultures of mutBI cells contain high numbers of spontaneous mutants and show hypermutability after exposure to the latter mutagen. Normally high-efficiency transforming markers, as well as low-efficiency ones, transform mutBI recipients at similarly low efficiencies. Significant host cell reactivation was observed when mutBI cells were exposed to UV-damaged phage; however, these mutants showed a decrease in phage recombination. This mutant did not degrade its DNA following exposure to UV. It is speculated that the mutBI mutation is similar to the Escherichia coli uvrD mutation.

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Repair deficiency in Escherichia coli UV-sensitive mutator strain uvr502

Cited by 27 publications

References 13 publications

The mutH, mutL, mutS, and uvrD Genes of Salmonella typhimurium LT2

The mutH, mutL, mutS, and uvrD Genes of Salmonella typhimurium LT2

Effect of the uvrD mutation on excision repair

Isolation and characterization of a UV-sensitive mutator (mutB1) mutant of Haemophilus influenzae

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