Renumbering the S-RNase alleles of European pears (Pyrus communis L.) and cloning the S109 RNase allele

Martin, Gottfried; Takasaki-Yasuda, Takashi; Sanzol, Javier; Mota, Mariana; Zisovich, Annat; Stern, Raphael A.; Sansavini, S.

doi:10.1016/j.scienta.2008.08.027

Cited by 45 publications

(20 citation statements)

References 35 publications

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“…The results obtained are conclusive and indicate that 'Belle lucrative' should be considered as an effective pollinizer in addition to 'Clara Frijs'. S-genotyping of 'Ingeborg' revealed that this cultivar possesses the following three S alleles: S102, S108, and S121, labeled in accordance with the numeration proposed by Goldway et al (2009). Considering 'Ingeborg', parent cultivar 'Conference' possesses S108 and S121 S alleles (Sanzol, 2009), whereas the other parent 'Bonne Louise' possesses S101 and S102 S alleles (Sanzol and Robbins, 2008), and the obtained S allele profile confirms the conclusion by Sehic et al (2012) that 'Ingeborg' is derived from an unreduced 'Conference' egg cell being fertilized by a haploid pollen cell from 'Bonne Louise'.…”

Section: Resultssupporting

confidence: 66%

Pollinizer Efficacy of Several ‘Ingeborg’ Pear Pollinizers in Hardanger, Norway, Examined Using Microsatellite Markers

Gaši¹,

Pojskić²,

Kurtović³

et al. 2017

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Section: Resultssupporting

confidence: 66%

Pollinizer Efficacy of Several ‘Ingeborg’ Pear Pollinizers in Hardanger, Norway, Examined Using Microsatellite Markers

Gaši¹,

Pojskić²,

Kurtović³

et al. 2017

horts

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“…Within the Pyrinae subtribe (formerly subfamily Maloideae; Potter et al 2007;Campbell et al 2007), many S-RNase alleles have been sequenced and molecular S-genotyping assays have been developed for the most agronomically important species, including apple (Janssens et al 1995;Verdoodt et al 1998;Matsumoto et al 1999aMatsumoto et al , b, 2003aKitahara et al 2000;Broothaerts 2003;Ershadi and Talaii 2007;Kim et al 2006Kim et al , 2009, European pear (Zuccherelli et al 2002;Zisovich et al 2004;Sanzol et al 2006;Takasaki et al 2006;Moriya et al 2007;Mota et al 2007;Sanzol and Robbins 2008;Goldway et al 2009;Sanzol 2009a, b), and Asian pears (Ishimizu et al 1999;Castillo et al 2001;Kim et al 2002Kim et al , 2007Takasaki et al 2004;Gu et al 2009). In the Pyrinae, pairs of S-RNase alleles showing high sequence similarity are commonly found, even among distantly related species (Raspé and Kohn 2002).…”

Section: Communicated By a Abbottmentioning

confidence: 99%

Evaluation of candidate F-box genes for the pollen S of gametophytic self-incompatibility in the Pyrinae (Rosaceae) on the basis of their phylogenomic context

Franceschi

Pierantoni

Dondini

et al. 2011

Tree Genetics & Genomes

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The recent analysis of the S-locus region of apple and Japanese pear, two species of Pyrinae (Rosaceae), suggested multiple and different F-box genes (called SFBBs) as candidates for the male determinant (pollen S) of RNase-based gametophytic self-incompatibility in these two species. Here, we followed a phylogenetic approach to take advantage of the pattern of molecular evolution of the S-locus of Pyrinae in characterizing SFBB homologs belonging to S-haplotypes of apple and three species of Pyrus (European, Japanese, and Chinese pears). Our results suggested that the S-locus region of Pyrinae contains no less than six SFBB members and that its structure seems to be rather conserved between apple and pear species. In accordance with the prevailing theory on S-haplotype evolution, the pollen S is expected to have coevolved with the S-RNase and to show some common features derived from the long-term evolution under frequency-dependent balancing selection, i.e., high sequence diversity, evidence of positive selection, and shared ancestral polymorphisms. Using this conceptual framework, we present evidence that some SFBB genes may be better candidates for pollen S in Pyrinae than others. Overall, the SFBB genes analyzed exhibited much lower sequence diversity than their associated S-RNases; likewise, they showed little or no evidence of positive selection. However, evidence of coevolution with the S-RNase clearly emerged for two of them. Altogether our results suggested different evolutionary histories for different SFBBs putatively derived from their distinct involvement in self-incompatibility.

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“…Since its discovery in the 1980s (Bredemeijer and Blaas 1981;Anderson et al 1986;McClure et al 1989), this protein and its coding gene have been intensively studied in different species. In the European pear, the S-RNase gene has been sequenced for 24 different S-haplotypes, and several Sgenotyping molecular assays have been developed on this gene (Zuccherelli et al 2002;Zisovich et al 2004;Sanzol et al 2006;Takasaki et al 2006;Moriya et al 2007;Mota et al 2007;Sanzol and Robbins 2008;Sanzol 2009a, b;Goldway et al 2009;Sanzol 2010).…”

Section: Introductionmentioning

confidence: 99%

Cloning and mapping multiple S-locus F-box genes in European pear (Pyrus communis L.)

Franceschi

Pierantoni

Dondini

et al. 2010

Tree Genetics & Genomes

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European pear, as well as its close relatives Japanese pear and apple, exhibits S-RNase-based gametophytic self-incompatibility. The male determinant of this self-incompatibility mechanism is a pollen-expressed protein containing an F-box domain; in the genera Petunia (Solanaceae), Antirrhinum (Plantaginaceae), and Prunus (Rosaceae), a single F-box gene determines the pollen S. In apple and Japanese pear, however, multiple S-locus F-box genes were recently identified as candidates for the pollen S, and they were named S-locus F-Box Brothers. These genes were considered good candidates for the pollen S determinant since they exhibit S-haplotype-specific polymorphisms, pollen-specific expression, and linkage to the S-RNase. In the present study, S-locus F-Box Brothers homologs have been cloned from two of the most agronomically important European pear varieties, "Abbé Fétel" (S 104-2 /S 105 ) and "Max Red Bartlett" (S 101 /S 102 ), and they have been mapped on a genetic linkage map developed on their progeny. Our results suggest that the number of Fbox genes linked to the S-locus of the European pear is higher than expected according with previous reports for apple and Japanese pear, since up to five genes were found to be linked to a single S-haplotype. Moreover, two of these genes exhibited an incomplete linkage to the S-RNase, allowing the identification of low-frequency recombinant haplotypes, generated by a crossing-over event between the two genes. These F-box genes are most likely placed in close proximity of the S-locus but do not belong to it, and they can thus be excluded from being responsible for the determination of pollen S function.

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Renumbering the S-RNase alleles of European pears (Pyrus communis L.) and cloning the S109 RNase allele

Cited by 45 publications

References 35 publications

Pollinizer Efficacy of Several ‘Ingeborg’ Pear Pollinizers in Hardanger, Norway, Examined Using Microsatellite Markers

Pollinizer Efficacy of Several ‘Ingeborg’ Pear Pollinizers in Hardanger, Norway, Examined Using Microsatellite Markers

Evaluation of candidate F-box genes for the pollen S of gametophytic self-incompatibility in the Pyrinae (Rosaceae) on the basis of their phylogenomic context

Cloning and mapping multiple S-locus F-box genes in European pear (Pyrus communis L.)

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