Cloning and mapping multiple S-locus F-box genes in European pear (Pyrus communis L.)

Franceschi, Paolo De; Pierantoni, Luca; Dondini, Luca; Grandi, Marco; Sanzol, Javier; Sansavini, S.

doi:10.1007/s11295-010-0327-5

Cited by 31 publications

(23 citation statements)

References 57 publications

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“…Map-based cloning and chromosome walking approaches to study the Solanaceae, Plantaginaceae, and Rosaceae S loci have commonly identified multiple pollen-expressed F-box genes flanking the S-RNase gene (Fig. 3;de Franceschi et al, 2011;Entani et al, 2003;Lai et al, 2002;Minamikawa et al, 2010;Okada et al, 2008Okada et al, , 2010Okada et al, , 2013Sassa et al, 2007;Ushijima et al, 2001Ushijima et al, , 2004Wang et al, 2003Wang et al, , 2004Wheeler and Newbigin, 2007;Zhou et al, 2003). Compared with that of the genus Prunus, the recombination-suppressed region around the S locus is much larger and more highly heterochromatic in the tribe Maleae and the families Solanaceae and Plantaginaceae, making it difficult to isolate the true pollen S gene in these three plant taxa (Entani et al, 1999a;Yang et al, 2007).…”

Section: Identification Of F-box Genes As the Pollen S Gene Candidatementioning

confidence: 99%

Distinct Self-recognition in the <i>Prunus</i> S-RNase-based Gametophytic Self-incompatibility System

Matsumoto

Tao

2016

The Hortic J

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Prunus fruit tree species exhibit S-ribonuclease (S-RNase)-based gametophytic self-incompatibility (GSI). This system is also present in the families Plantaginaceae and Solanaceae and the tribe Maleae of the family Rosaceae. In S-RNase-based GSI, self/nonself-recognition between the pistil and pollen is controlled by the pistil S determinant S-ribonuclease gene (S-RNase) and the pollen S determinant F-box gene(s). Accumulated evidence indicates the Prunus pollen S locus contains a single F-box gene, while that of other plants consists of multiple F-box genes. The pollen S F-box genes are called S haplotype-specific F-box (SFB), S-locus F-box brothers (SFBB), and S-locus F-box (SLF) in Prunus, Maleae, and Solanaceae species, respectively. The consequences of pollen S gene mutations and heterodiallelic pollen production differ between Prunus species and other plants, suggesting there are different pollen S functions during self/nonself-recognition. The GSI systems of Prunus and other plants are believed to include the ubiquitin proteasome system for protein degradation. However, Prunus SFB is assumed to facilitate the S-RNase cytotoxic effects during selfrecognition, while SLFs and SFBBs are thought to function collaboratively during nonself-recognition to avoid S-RNase cytotoxicity. This review summarizes the distinct features of the S-RNase-based GSI mechanism in Prunus species, with special references to the recent advances in our understanding of S-RNase-based GSI.

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Section: Identification Of F-box Genes As the Pollen S Gene Candidatementioning

confidence: 99%

Distinct Self-recognition in the <i>Prunus</i> S-RNase-based Gametophytic Self-incompatibility System

Matsumoto

Tao

2016

The Hortic J

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“…In an accompanying paper, we have characterized SFBB homologs in the European pear (Pyrus communis L.) and have analyzed their genetic linkage to the S-RNase (De Franceschi et al 2010). The results presented in that paper suggested that the actual number of SFBB-related genes present at the S-locus region of the European pear is higher than the number of genes initially reported for the S-locus of the apple and the Japanese pear (P. pyrifolia Nakai) .…”

Section: Communicated By a Abbottmentioning

confidence: 99%

Evaluation of candidate F-box genes for the pollen S of gametophytic self-incompatibility in the Pyrinae (Rosaceae) on the basis of their phylogenomic context

Franceschi

Pierantoni

Dondini

et al. 2011

Tree Genetics & Genomes

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The recent analysis of the S-locus region of apple and Japanese pear, two species of Pyrinae (Rosaceae), suggested multiple and different F-box genes (called SFBBs) as candidates for the male determinant (pollen S) of RNase-based gametophytic self-incompatibility in these two species. Here, we followed a phylogenetic approach to take advantage of the pattern of molecular evolution of the S-locus of Pyrinae in characterizing SFBB homologs belonging to S-haplotypes of apple and three species of Pyrus (European, Japanese, and Chinese pears). Our results suggested that the S-locus region of Pyrinae contains no less than six SFBB members and that its structure seems to be rather conserved between apple and pear species. In accordance with the prevailing theory on S-haplotype evolution, the pollen S is expected to have coevolved with the S-RNase and to show some common features derived from the long-term evolution under frequency-dependent balancing selection, i.e., high sequence diversity, evidence of positive selection, and shared ancestral polymorphisms. Using this conceptual framework, we present evidence that some SFBB genes may be better candidates for pollen S in Pyrinae than others. Overall, the SFBB genes analyzed exhibited much lower sequence diversity than their associated S-RNases; likewise, they showed little or no evidence of positive selection. However, evidence of coevolution with the S-RNase clearly emerged for two of them. Altogether our results suggested different evolutionary histories for different SFBBs putatively derived from their distinct involvement in self-incompatibility.

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“…Although the Prunus pollen S was originally referred to by two different terms, SFB and SLF (Entani et al, 2003), we use SFB in this article because it distinguishes the Prunus system from other systems, and recent studies have all used SFB (Newbigin et al, 2008;Sassa et al, 2010;Tao and Iezzoni, 2010). The S locus F-box brothers (SFBBs) have been identified as candidates for the pollen S determinant in the subtribe Pyrinae of the Rosaceae (De Franceschi et al, 2011a, 2011bKakui et al, 2011;Minamikawa et al, 2010;Okada et al, 2008Okada et al, , 2011Sassa et al, 2007). Variants of the S locus defined by combinations of molecular mechanism of GSI, possibly because unidentified pollen and/or pistil factors play important roles in the SI/SC reaction.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis of Self- and Cross-pollinated Pistils of Japanese Apricot (Prunus mume Sieb. et Zucc.)

Habu

Tao

2014

J. Japan. Soc. Hort. Sci.

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Solanaceae, Rosaceae, and Plantaginaceae exhibit the S-RNase-based gametophytic self-incompatibility (GSI) system. This type of GSI is controlled by a single polymorphic locus (S locus) containing the pistil S determinant gene, S-ribonuclease (S-RNase), and the pollen S determinant, the S locus F-box gene (SFB/SLF). In addition to these determinant genes, non-S factors, called modifier genes, are required for the GSI reaction. Here, we conducted large-scale transcriptome analysis of unpollinated, self-pollinated, and cross-pollinated pistils of Japanese apricot (Prunus mume Sieb. et Zucc. cv. Nanko) to capture all of the molecular events induced by the GSI reaction in Prunus, using next-generation sequencing technologies. We obtained 40,061 unigenes from 77,521,310 reads from pollinated and unpollinated pistils and pollen grains. Among these unigenes, 29,985 and 27,898 unigene sequences showed at least one hit against the NCBI nr and TAIR10 protein databases, respectively, in BLASTX searches using an E-value cutoff of 1e-6. Digital expression analysis showed that 8,907 and 10,190 unigenes were expressed at significantly different levels between unpollinated (UP) and cross-pollinated (CP) pistils and between UP and self-pollinated (SP) pistils, respectively. The expression of 4,348 unigenes in both CP and SP pollination was commonly and significantly different from that in UP, while the expression of 4,559 and 5,842 unigenes in CP and SP, respectively, was specifically and significantly different from UP. The expression of 2,227 unigenes was up-regulated both in CP and SP compared with UP. Genes supposedly involved in S-RNasebased GSI were included among the unigenes up-regulated by pollination, while no unigenes homologous to the solanaceous pistil modifiers HT-B or 120K were included among the unigenes up-regulated by pollination or in the whole unpollinated/pollinated pistil transcriptome. We discuss the distinct molecular mechanism of S-RNase-based GSI in Prunus.

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Cloning and mapping multiple S-locus F-box genes in European pear (Pyrus communis L.)

Cited by 31 publications

References 57 publications

Distinct Self-recognition in the <i>Prunus</i> S-RNase-based Gametophytic Self-incompatibility System

Distinct Self-recognition in the <i>Prunus</i> S-RNase-based Gametophytic Self-incompatibility System

Evaluation of candidate F-box genes for the pollen S of gametophytic self-incompatibility in the Pyrinae (Rosaceae) on the basis of their phylogenomic context

Transcriptome Analysis of Self- and Cross-pollinated Pistils of Japanese Apricot (Prunus mume Sieb. et Zucc.)

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