RENEB intercomparisons applying the conventional Dicentric Chromosome Assay (DCA)

Oestreicher, Ursula; Samaga, Daniel; Ainsbury, Elizabeth A.; Antunes, Ana; Baeyens, Ans; Barrios, Leonardo; Beinke, Christina; Beukes, Philip; Blakely, William F.; Cucu, Alexandra; Amicis, Andrea De; Depuydt, Julie; Sanctis, Stefania De; Giorgio, Marina Di; Dobos, Katalin; Domı́nguez, I.; Duy, Pham Ngoc; Espinoza, Miguel; Flegal, Farrah; Figel, M.; García, Omar; Gil, Octávia Monteiro; Grégoire, Éric; Guerrero-Carbajal, C.; Güçlü, İnci; Hadjidekova, Valeria; Hande, M. Prakash; Kulka, Ulrike; Lemon, Jennifer A.; Lindholm, Carita; Lista, Florigio; Lumniczky, Katalin; Martínez‐López, Wilner; Maznyk, Nataliya; Meschini, Roberta; M’kacher, Radia; Montoro, Alegría; Moquet, Jayne; Moreno, M. Echevarría; Noditi, Mihaela; Pajić, Jelena; Radl, A.; Ricoul, Michelle; Romm, Horst; Roy, Laurence; Sabatier, Laure; Sebastià, Natividad; Slabbert, Jacobus; Sommer, Sylwester; Oliveira, Monica Stuck de; Subramanian, Uma; Suto, Yumiko; Que, Tran; Testa, Antonella; Terzoudi, Georgia I.; Vral, Anne; Wilkins, Ruth; Lusiyanti, Yanti; Zafiropoulos, D.; Wójcik, Andrzej

doi:10.1080/09553002.2016.1233370

Cited by 81 publications

(85 citation statements)

References 21 publications

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“…The results of such inter-comparisons have been analysed differently according to the network (Garcıa et al 2013;Depuydt et al 2013;Bassinet et al 2014;Fattibene et al 2014;Barnard et al 2015;Wilkins et al 2015;Moquet et al 2016;Oestreicher et al 2016). It is therefore important to fix the adapted criteria for inter-comparison analysis as it can have an impact on the conclusion of the proficiency of a laboratory.…”

Section: Discussionmentioning

confidence: 99%

“…Training courses were mainly dedicated to DCA, FISH, MN, cH2AX and PCC assays. After completion of the practical training, a second inter-comparison was performed also on irradiated samples Oestreicher et al 2016;Terzoudi et al 2016). The results showed the benefits of training sessions.…”

Section: Education and Trainingmentioning

confidence: 99%

“…A first inter-comparison on biological and physical dosimetry allowed identification of laboratories requiring training on specific assays Oestreicher et al 2016;Trompier et al 2016a). Furthermore, some laboratories required training on assay(s) that they do not master.…”

Section: Education and Trainingmentioning

confidence: 99%

“…The first intercomparison focused on biological assays (DCA; FISH; MN and PCC) has shown that the majority of RENEB partners reached the criteria for biological dosimetry indicator scoring and dose estimation: the calculated 95% confidence interval, associated to the estimated dose, should contain the physical dose delivered to the sample Oestreicher et al 2016). However, deviations from the statistically approved intervals for the dicentric (DCA), translocation detection by Fluorescence in situ Hybridization (FISH), micronucleus (MN) and Premature Condensed Chromosome (PCC) (Terzoudi et al 2016) assays were observed for a few laboratories, indicating the requirement for training for these partners.…”

Section: Education and Trainingmentioning

confidence: 99%

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The harmonization process to set up and maintain an operational biological and physical retrospective dosimetry network: QA QM applied to the RENEB network

Grégoire

Ainsbury

Barrios

et al. 2016

International Journal of Radiation Biology

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Purpose: The European Network of Biological and Physical Retrospective Dosimetry 'RENEB' has contributed to European radiation emergency preparedness. To give homogeneous dose estimation results, RENEB partners must harmonize their processes. Materials and methods: A first inter-comparison focused on biological and physical dosimetry was used to detect the outliers in terms of dose estimation. Subsequently, trainings were organized to improve both tools dose estimation. A second inter-comparison was performed to validate training efficiency. Simultaneously, based on ISO standards, a QA&QM manual on all dosimetry assays was produced which states a common basis and harmonized procedures for each assay. The evaluation of the agreement of RENEB partners to follow the QA&QM manual was performed through a questionnaire. The integration of new members into the network was carried out in the same way, whatever the assays. Results: The training courses on biological and physical dosimetry were judged to be successful because most of the RENEB members' dose estimates improved in the second inter-comparison. The QA&QM manual describes the consensus for the minimum requirements and the performance criteria for both dosimetry assays. The questionnaire revealed that the whole network capacity currently can manage between 15 and 3800 samples once. Conclusion:The methodology used to harmonize all dosimetry practice within the network RENEB was highly successful. The network is operational to manage a mass casualty radiation accident for immediate dose assessment. ARTICLE HISTORY

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Section: Discussionmentioning

confidence: 99%

Section: Education and Trainingmentioning

confidence: 99%

Section: Education and Trainingmentioning

confidence: 99%

Section: Education and Trainingmentioning

confidence: 99%

See 2 more Smart Citations

The harmonization process to set up and maintain an operational biological and physical retrospective dosimetry network: QA QM applied to the RENEB network

Grégoire

Ainsbury

Barrios

et al. 2016

International Journal of Radiation Biology

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“…For the second ILC, all samples were irradiated in air at the same facility with dosimetric references expressed in terms of air kerma, whereas some participants reported results in terms of absorbed dose in water. This fact is not taken into account in the analysis provided in papers submitted in this issue Oestreicher et al 2016;Terzoudi et al 2016). …”

Section: Re-evaluation Of Data Of the Second Ilcmentioning

confidence: 99%

Investigation of the influence of calibration practices on cytogenetic laboratory performance for dose estimation

Trompier

Baumann

Barrios

et al. 2016

International Journal of Radiation Biology

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Purpose: In the frame of the QA program of RENEB, an inter-laboratory comparison (ILC) of calibration sources used in biological dosimetry was achieved to investigate the influence of calibration practices and protocols on the results of the dose estimation performance as a first step to harmonization and standardization of dosimetry and irradiation practices in the European biological dosimetry network. Materials and methods: Delivered doses by irradiation facilities used by RENEB partners were determined with EPR/alanine dosimetry system. Dosimeters were irradiated in the same conditions as blood samples. A short survey was also performed to collect the information needed for the data analysis and evaluate the diversity of practices. Results: For most of partners the deviation of delivered dose from the targeted dose remains below 10%. Deviations larger than 10% were observed for five facilities out of 21. Origins of the largest discrepancies were identified. Correction actions were evaluated as satisfactory. The re-evaluation of some ILC results for the fluorescence in situ hybridization (FISH) and premature chromosome condensation (PCC) assays has been performed leading to an improvement of the overall performances. Conclusions: This work has shown the importance of dosimetry in radiobiology studies and the needs of harmonization, standardization in irradiation and dosimetry practices and educational training for biologists using ionizing radiation. ARTICLE HISTORY

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Does proliferation capacity of lymphocytes depend on human blood types?

Viswanathan

Kanagaraj

Raavi

et al. 2018

J of Cellular Biochemistry

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In vitro human lymphocyte culture methodology is well established yet certain confounding factors such as age, medical history as well as individual’s blood type may potentially modulate in vitro proliferation response. These factors have to be carefully evaluated to release reliable test report in routine cytogenetic evaluation for various genetic conditions, radiation biodosimetry, etc. With this objective, the current study was focused on analyzing the proliferation response of lymphocytes drawn from 90 individuals (21‐29 years) with different blood types. The proliferation response was assessed in the cultured lymphocytes by cell cycle, mitotic index (MI), and nuclear division index (NDI) after stimulation with phytohaemagglutinin (PHA). To investigate the toxic effect on proliferation, MI was calculated in representative samples of each blood type were X‐irradiated. The results showed that there was no significant difference among the cell cycle phases of lymphocytes in different blood types (P > 0.05). Similarly, both MI and NDI of lymphocytes derived from different blood types also did not show significant difference ( P > 0.05). The extensive interindividual variation within and among the blood types is likely responsible for the lack of significant difference in lymphocyte proliferation. Although spontaneous proliferation efficiency of lymphocytes of different blood types after PHA stimulation was grossly similar, the MI observed after radiation exposure showed a significant difference ( P < 0.05) indicating a differential proliferation response among the blood types. Our results suggest that the blood types did not have any impact on PHA‐induced proliferation; however, a specific differential lymphocyte proliferation observed after radiation exposure needs to be considered.

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RENEB intercomparisons applying the conventional Dicentric Chromosome Assay (DCA)

Cited by 81 publications

References 21 publications

The harmonization process to set up and maintain an operational biological and physical retrospective dosimetry network: QA QM applied to the RENEB network

The harmonization process to set up and maintain an operational biological and physical retrospective dosimetry network: QA QM applied to the RENEB network

Investigation of the influence of calibration practices on cytogenetic laboratory performance for dose estimation

Does proliferation capacity of lymphocytes depend on human blood types?

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