Renaturation of DNA catalysed by yeast DNA repair and recombination protein RAD1O

Sung, Patrick; Prakash, Louise; Prakash, Satya

doi:10.1038/355743a0

Cited by 39 publications

(27 citation statements)

References 24 publications

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“…Supporting this idea is the fact that the Rad1 protein is a component of the Rad1/Rad10 protein complex possessing DNA endonuclease activity specific for 3Ј-ended tails (3,6,8,57,60,61). Recently, additional, and different, biochemical activities have been assigned to the Rad1 and Rad10 proteins (23,56). Rad10 was shown to promote the renaturation of complementary DNA strands (56), and Rad1 was reported to catalyze cleavage of a synthetic Holliday junction (23; but see reference 66).…”

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confidence: 88%

“…1e). Rad10 was previously shown to promote the renaturation of complementary DNA strands (56). Thus, the possibility that Rad10 also functions at an earlier step of the SSA process cannot be formally excluded.…”

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confidence: 98%

“…Recently, additional, and different, biochemical activities have been assigned to the Rad1 and Rad10 proteins (23,56). Rad10 was shown to promote the renaturation of complementary DNA strands (56), and Rad1 was reported to catalyze cleavage of a synthetic Holliday junction (23; but see reference 66).…”

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confidence: 99%

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RAD1 and RAD10, but Not Other Excision Repair Genes, Are Required for Double-Strand Break-Induced Recombination in Saccharomyces cerevisiae

Ivanov

Haber

1995

Molecular and Cellular Biology

209

158

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HO endonuclease-induced double-strand breaks (DSBs) in the yeast Saccharomyces cerevisiae can be repaired by the process of gap repair or, alternatively, by single-strand annealing if the site of the break is flanked by directly repeated homologous sequences. We have shown previously (J. Fishman-Lobell and J. E. Haber, Science 258:480-484, 1992) that during the repair of an HO-induced DSB, the excision repair gene RAD1 is needed to remove regions of nonhomology from the DSB ends. In this report, we present evidence that among nine genes involved in nucleotide excision repair, only RAD1 and RAD10 are required for removal of nonhomologous sequences from the DSB ends. rad1⌬ and rad10⌬ mutants displayed a 20-fold reduction in the ability to execute both gap repair and single-strand annealing pathways of HO-induced recombination. Mutations in RAD2, RAD3, and RAD14 reduced HO-induced recombination by about twofold. We also show that RAD7 and RAD16, which are required to remove UV photodamage from the silent HML locus, are not required for MAT switching with HML or HMR as a donor. Our results provide a molecular basis for understanding the role of yeast nucleotide excision repair genes and their human homologs in DSB-induced recombination and repair.Genes belonging to the epistasis group RAD3 are required for nucleotide excision repair (NER) of UV-damaged DNA in the yeast Saccharomyces cerevisiae (for a recent review, see reference 40). Some of these genes also function in other pathways of DNA metabolism. Genomic deletions of RAD3 (34) and SSL2 (RAD25) (17, 38) are lethal in haploid cells, indicating their essential role in maintaining cell viability. Experiments using conditional lethal mutations of RAD3 and SSL2 have demonstrated that their essential function is most likely their role in RNA polymerase II transcription (18,19,41). Substantiating the idea of an intimate linkage between NER and transcription were recent findings that protein products of at least RAD2, RAD3, RAD4, and SSL2 are components of or are physically associated with RNA polymerase II basal transcription factor b (5, 12).Experimental data also show that some NER genes have a role in mitotic recombination. Mutations in RAD1 and RAD10 reduce intrachromosomal recombination between directly repeated sequences and decrease the efficiency of homologous integration of linear DNA fragments and circular plasmids (30,45,47,48). The RAD1 function is also required for elevated recombination in cdc9 and top3 mutants (2, 47) as well as for mitotic recombination stimulated by RNA polymerase I-or II-dependent transcription (59, 67). The effect of rad1 and rad10 mutations on recombination seems to be rather specific in the sense that mitotic, but not meiotic, events are affected in the mutant strains (48). Moreover, only rad1 and rad10, not other NER mutants, showed defects in spontaneous recombination between directly repeated genes (47).A key to understanding a precise role of RAD1 in mitotic recombination came from our previous studies on the genetic control ...

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RAD1 and RAD10, but Not Other Excision Repair Genes, Are Required for Double-Strand Break-Induced Recombination in Saccharomyces cerevisiae

Ivanov

Haber

1995

Molecular and Cellular Biology

209

158

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“…The RAD10 protein binds to both single-and double-stranded DNA at pH 7.5 (7). At pH 6, RAD10 exhibits a preference for single-stranded DNA and can promote the aggregation of complementary single strands (46). Yeast strains carrying radl (27,39,48,58) or radlO (40) loss-of-function alleles exhibit a reduction in certain mitotic recombination events.…”

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confidence: 99%

“…In S. cerevisiae only the RAD3 (23,47) and RAD10 proteins (7,46) have been extensively purified. The RAD3 protein is a DNA-dependent ATPase with associated processive DNA-DNA helicase (23,45,47) and DNA-RNA helicase activities (5,33).…”

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Stable and specific association between the yeast recombination and DNA repair proteins RAD1 and RAD10 in vitro.

Bardwell¹,

Cooper²,

Friedberg³

1992

Mol. Cell. Biol.

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The RAD1 and RADIO genes of Saccharomyces cerevisiae are two of at least seven genes which are known to be required for damage-specific recognition and/or damage-specific incision of DNA during nucleotide excision repair. RADi and RADIO are also involved in a specialized mitotic recombination pathway. We have previously reported the purification of the RADIO protein to homogeneity (L. Bardwell, H. Burtscher, W. A. Weiss, C. M. Nicolet, and E. C. Friedberg, Biochemistry 29:3119-3126, 1990). In the present studies we show that the RAD1 protein, produced by in vitro transcription and translation of the cloned gene, specifically coimmunoprecipitates with the RADI10 protein translated in vitro or purified from yeast. Conversely, in vitro-translated RADIO protein specifically coimmunoprecipitates with the RADI protein. The sites of this stable and specific interaction have been mapped to the C-terminal regions of both polypeptides. This portion of RADI10 protein is evolutionarily conserved. These results are the first biochemical evidence of a specific association between any eukaryotic proteins genetically identified as belonging to a recombination or DNA repair pathway and suggest that the RAD1 and RAD10 proteins act at the same or consecutive biochemical steps in both nucleotide excision repair and mitotic recombination.In the budding yeast Saccharomyces cerevisiae, the re-

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Genes controlling nucleotide excision repair in eukaryotic cells

1993

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The maintenance of genetic integrity is of vital importance to all living organisms. However, DNA--the carrier of genetic information--is continuously subject to damage induced by numerous agents from the environment and endogenous cellular metabolites. To prevent the deleterious consequences of DNA injury, an intricate network of repair systems has evolved. The biological impact of these repair mechanisms is illustrated by a number of genetic diseases that are characterized by a defect in one of the repair machineries and in general predispose individuals to cancer. This article intends to review our current understanding of the complex nucleotide excision repair pathway, a universal repair system with a broad lesion specificity. Emphasis will be on the recent advances in the genetic analysis of this process in mammalian cells.

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Renaturation of DNA catalysed by yeast DNA repair and recombination protein RAD1O

Cited by 39 publications

References 24 publications

RAD1 and RAD10, but Not Other Excision Repair Genes, Are Required for Double-Strand Break-Induced Recombination in Saccharomyces cerevisiae

RAD1 and RAD10, but Not Other Excision Repair Genes, Are Required for Double-Strand Break-Induced Recombination in Saccharomyces cerevisiae

Stable and specific association between the yeast recombination and DNA repair proteins RAD1 and RAD10 in vitro.

Genes controlling nucleotide excision repair in eukaryotic cells

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